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Developmental relationship between cytotoxic alpha/beta T cell receptor-positive intraepithelial lymphocytes and Peyer's patch lymphocytes.
Eur J Immunol. 1993 Jun; 23(6):1333-9.EJ

Abstract

Following intraduodenal priming of mice with reovirus, precursor cytotoxic T lymphocytes (pCTL) rapidly appear in intraepithelial lymphocytes (IEL) and Peyer's patches. These cells express CTL activity after secondary in vitro stimulation with reovirus-infected cells. Adoptive transfer of Peyer's patch lymphocytes from normal BALB/c mice into reovirus-infected CB.17 severe combined immunodeficiency mice results in the infection-dependent appearance of large numbers of both CD8+Thy-1+ and CD8-Thy-1+, IEL that express the alpha/beta T cell receptor (TcR). Phenotypic and functional characterization of IEL derived from conventionally reared, reovirus-infected mice also points to extensive similarities in the pCTL derived from Peyer's patches and IEL. As in the Peyer's patches, pCTL are persistent in the IEL compartment for up to 4 weeks after infection. A large percentage of IEL that are recovered from reovirus-primed mice after in vitro culture are CD8+Thy-1+ cells that express alpha/beta TcR. Furthermore, depletion experiments demonstrate that the CD8+Thy-1+ population mediates the virus-specific CTL activity. Using limiting dilution analyses, it was estimated that 7 days after intraduodenal infection the average frequency of virus-specific pCTL was 197/10(6) CD8+Thy-1+ IEL and 190/10(6) CD8+Thy-1+ Peyer's patch lymphocytes. Taken together, these observations provide evidence that specific cellular immunity to reovirus in IEL is mediated at least in part, by conventional cytotoxic T lymphocytes and that these cells are functionally and phenotypically similar to the pCTL derived from the Peyer's patches.

Authors+Show Affiliations

Department of Biology, University of Pennsylvania, Philadelphia 19104.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8388798

Citation

Cuff, C F., et al. "Developmental Relationship Between Cytotoxic Alpha/beta T Cell Receptor-positive Intraepithelial Lymphocytes and Peyer's Patch Lymphocytes." European Journal of Immunology, vol. 23, no. 6, 1993, pp. 1333-9.
Cuff CF, Cebra CK, Rubin DH, et al. Developmental relationship between cytotoxic alpha/beta T cell receptor-positive intraepithelial lymphocytes and Peyer's patch lymphocytes. Eur J Immunol. 1993;23(6):1333-9.
Cuff, C. F., Cebra, C. K., Rubin, D. H., & Cebra, J. J. (1993). Developmental relationship between cytotoxic alpha/beta T cell receptor-positive intraepithelial lymphocytes and Peyer's patch lymphocytes. European Journal of Immunology, 23(6), 1333-9.
Cuff CF, et al. Developmental Relationship Between Cytotoxic Alpha/beta T Cell Receptor-positive Intraepithelial Lymphocytes and Peyer's Patch Lymphocytes. Eur J Immunol. 1993;23(6):1333-9. PubMed PMID: 8388798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental relationship between cytotoxic alpha/beta T cell receptor-positive intraepithelial lymphocytes and Peyer's patch lymphocytes. AU - Cuff,C F, AU - Cebra,C K, AU - Rubin,D H, AU - Cebra,J J, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 1333 EP - 9 JF - European journal of immunology JO - Eur J Immunol VL - 23 IS - 6 N2 - Following intraduodenal priming of mice with reovirus, precursor cytotoxic T lymphocytes (pCTL) rapidly appear in intraepithelial lymphocytes (IEL) and Peyer's patches. These cells express CTL activity after secondary in vitro stimulation with reovirus-infected cells. Adoptive transfer of Peyer's patch lymphocytes from normal BALB/c mice into reovirus-infected CB.17 severe combined immunodeficiency mice results in the infection-dependent appearance of large numbers of both CD8+Thy-1+ and CD8-Thy-1+, IEL that express the alpha/beta T cell receptor (TcR). Phenotypic and functional characterization of IEL derived from conventionally reared, reovirus-infected mice also points to extensive similarities in the pCTL derived from Peyer's patches and IEL. As in the Peyer's patches, pCTL are persistent in the IEL compartment for up to 4 weeks after infection. A large percentage of IEL that are recovered from reovirus-primed mice after in vitro culture are CD8+Thy-1+ cells that express alpha/beta TcR. Furthermore, depletion experiments demonstrate that the CD8+Thy-1+ population mediates the virus-specific CTL activity. Using limiting dilution analyses, it was estimated that 7 days after intraduodenal infection the average frequency of virus-specific pCTL was 197/10(6) CD8+Thy-1+ IEL and 190/10(6) CD8+Thy-1+ Peyer's patch lymphocytes. Taken together, these observations provide evidence that specific cellular immunity to reovirus in IEL is mediated at least in part, by conventional cytotoxic T lymphocytes and that these cells are functionally and phenotypically similar to the pCTL derived from the Peyer's patches. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/8388798/Developmental_relationship_between_cytotoxic_alpha/beta_T_cell_receptor_positive_intraepithelial_lymphocytes_and_Peyer's_patch_lymphocytes_ L2 - https://doi.org/10.1002/eji.1830230622 DB - PRIME DP - Unbound Medicine ER -