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Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187.
Br J Pharmacol. 1979 Jan; 65(1):103-9.BJ

Abstract

The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylnydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation.

Authors

No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

83892

Citation

Johansen, T. "Utilization of Adenosine Triphosphate in Rat Mast Cells During Histamine Release Induced By the Ionophore A23187." British Journal of Pharmacology, vol. 65, no. 1, 1979, pp. 103-9.
Johansen T. Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187. Br J Pharmacol. 1979;65(1):103-9.
Johansen, T. (1979). Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187. British Journal of Pharmacology, 65(1), 103-9.
Johansen T. Utilization of Adenosine Triphosphate in Rat Mast Cells During Histamine Release Induced By the Ionophore A23187. Br J Pharmacol. 1979;65(1):103-9. PubMed PMID: 83892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187. A1 - Johansen,T, PY - 1979/1/1/pubmed PY - 1979/1/1/medline PY - 1979/1/1/entrez SP - 103 EP - 9 JF - British journal of pharmacology JO - Br J Pharmacol VL - 65 IS - 1 N2 - The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylnydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/83892/Utilization_of_adenosine_triphosphate_in_rat_mast_cells_during_histamine_release_induced_by_the_ionophore_A23187_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1188&date=1979&volume=65&issue=1&spage=103 DB - PRIME DP - Unbound Medicine ER -