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Distribution of lens sodium-potassium-adenosine triphosphatase.
Invest Ophthalmol Vis Sci. 1993 Jun; 34(7):2159-63.IO

Abstract

PURPOSE

The specific activity of sodium-potassium-adenosine triphosphatase (Na-K-ATPase) in lens fiber cells is lower than the specific activity in lens epithelium. To test whether there is a reduction in the expression of Na-K-ATPase molecules in lens fibers, a Western blot technique was used.

METHODS

Membrane material was isolated from different regions of the rabbit lens. Na-K-ATPase (adenosine triphosphate hydrolysis) activity was measured in each membrane sample and Western blots were performed using an antibody to rabbit kidney Na-K-ATPase.

RESULTS

By immunoblotting, Na-K-ATPase polypeptide was detected in all lens cells. In contrast, adenosine triphosphate hydrolysis by the Na-K-ATPase (Na-K-ATPase activity) was not detectable or was detectable only at very low levels in fiber membranes from the lens nucleus and in cortex.

CONCLUSION

These findings suggest that plasma membrane adenosine triphosphatase enzyme responsible for sodium-potassium transport is expressed in newly formed lens fibers and the transport molecules are retained as the fibers age and are compressed toward the center of the lens. However, with fiber aging there is a loss of functional ability of the Na-K-ATPase to hydrolyze adenosine triphosphate.

Authors+Show Affiliations

Kentucky Lions Eye Research Institute, Louisville.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8389341

Citation

Delamere, N A., and W L. Dean. "Distribution of Lens Sodium-potassium-adenosine Triphosphatase." Investigative Ophthalmology & Visual Science, vol. 34, no. 7, 1993, pp. 2159-63.
Delamere NA, Dean WL. Distribution of lens sodium-potassium-adenosine triphosphatase. Invest Ophthalmol Vis Sci. 1993;34(7):2159-63.
Delamere, N. A., & Dean, W. L. (1993). Distribution of lens sodium-potassium-adenosine triphosphatase. Investigative Ophthalmology & Visual Science, 34(7), 2159-63.
Delamere NA, Dean WL. Distribution of Lens Sodium-potassium-adenosine Triphosphatase. Invest Ophthalmol Vis Sci. 1993;34(7):2159-63. PubMed PMID: 8389341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of lens sodium-potassium-adenosine triphosphatase. AU - Delamere,N A, AU - Dean,W L, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 2159 EP - 63 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 34 IS - 7 N2 - PURPOSE: The specific activity of sodium-potassium-adenosine triphosphatase (Na-K-ATPase) in lens fiber cells is lower than the specific activity in lens epithelium. To test whether there is a reduction in the expression of Na-K-ATPase molecules in lens fibers, a Western blot technique was used. METHODS: Membrane material was isolated from different regions of the rabbit lens. Na-K-ATPase (adenosine triphosphate hydrolysis) activity was measured in each membrane sample and Western blots were performed using an antibody to rabbit kidney Na-K-ATPase. RESULTS: By immunoblotting, Na-K-ATPase polypeptide was detected in all lens cells. In contrast, adenosine triphosphate hydrolysis by the Na-K-ATPase (Na-K-ATPase activity) was not detectable or was detectable only at very low levels in fiber membranes from the lens nucleus and in cortex. CONCLUSION: These findings suggest that plasma membrane adenosine triphosphatase enzyme responsible for sodium-potassium transport is expressed in newly formed lens fibers and the transport molecules are retained as the fibers age and are compressed toward the center of the lens. However, with fiber aging there is a loss of functional ability of the Na-K-ATPase to hydrolyze adenosine triphosphate. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/8389341/Distribution_of_lens_sodium_potassium_adenosine_triphosphatase_ L2 - https://iovs.arvojournals.org/article.aspx?volume=34&issue=7&page=2159 DB - PRIME DP - Unbound Medicine ER -