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Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression.
Mol Microbiol 1993; 8(2):205-10MM

Abstract

Endogenously synthesized trehalose is a stress protectant in Escherichia coli. Externally supplied trehalose does not serve as a stress protectant, but it can be utilized as the sole source of carbon and energy. Mutants defective in trehalose synthesis display an impaired osmotic tolerance in minimal growth media without glycine betaine, and an impaired stationary-phase-induced heat tolerance. Mechanisms for stress protection by trehalose are discussed. The genes for trehalose-6-phosphate synthase (otsA) and anabolic trehalose-6-phosphate phosphatase (otsB) constitute an operon. Their expression is induced both by osmotic stress and by growth into the stationary phase and depend on the sigma factor encoded by rpoS (katF). rpoS is amber-mutated in E. coli K-12 and its DNA sequence varies among K-12 strains. For trehalose catabolism under osmotic stress E. coli depends on the osmotically inducible periplasmic trehalase (TreA). In the absence of osmotic stress, trehalose induces the formation of an enzyme IITre (TreB) of the group translocation system, a catabolic trehalose-6-phosphate phosphatase (TreE), and an amylotrehalase (TreC) which converts trehalose to free glucose and a glucose polymer.

Authors+Show Affiliations

Norwegian College of Fishery Science, University of Tromsø.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

8391102

Citation

Strøm, A R., and I Kaasen. "Trehalose Metabolism in Escherichia Coli: Stress Protection and Stress Regulation of Gene Expression." Molecular Microbiology, vol. 8, no. 2, 1993, pp. 205-10.
Strøm AR, Kaasen I. Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression. Mol Microbiol. 1993;8(2):205-10.
Strøm, A. R., & Kaasen, I. (1993). Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression. Molecular Microbiology, 8(2), pp. 205-10.
Strøm AR, Kaasen I. Trehalose Metabolism in Escherichia Coli: Stress Protection and Stress Regulation of Gene Expression. Mol Microbiol. 1993;8(2):205-10. PubMed PMID: 8391102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression. AU - Strøm,A R, AU - Kaasen,I, PY - 1993/4/1/pubmed PY - 1993/4/1/medline PY - 1993/4/1/entrez SP - 205 EP - 10 JF - Molecular microbiology JO - Mol. Microbiol. VL - 8 IS - 2 N2 - Endogenously synthesized trehalose is a stress protectant in Escherichia coli. Externally supplied trehalose does not serve as a stress protectant, but it can be utilized as the sole source of carbon and energy. Mutants defective in trehalose synthesis display an impaired osmotic tolerance in minimal growth media without glycine betaine, and an impaired stationary-phase-induced heat tolerance. Mechanisms for stress protection by trehalose are discussed. The genes for trehalose-6-phosphate synthase (otsA) and anabolic trehalose-6-phosphate phosphatase (otsB) constitute an operon. Their expression is induced both by osmotic stress and by growth into the stationary phase and depend on the sigma factor encoded by rpoS (katF). rpoS is amber-mutated in E. coli K-12 and its DNA sequence varies among K-12 strains. For trehalose catabolism under osmotic stress E. coli depends on the osmotically inducible periplasmic trehalase (TreA). In the absence of osmotic stress, trehalose induces the formation of an enzyme IITre (TreB) of the group translocation system, a catabolic trehalose-6-phosphate phosphatase (TreE), and an amylotrehalase (TreC) which converts trehalose to free glucose and a glucose polymer. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/8391102/Trehalose_metabolism_in_Escherichia_coli:_stress_protection_and_stress_regulation_of_gene_expression_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1993&volume=8&issue=2&spage=205 DB - PRIME DP - Unbound Medicine ER -