Tags

Type your tag names separated by a space and hit enter

Frequent latent Epstein-Barr virus infection of neoplastic T cells and bystander B cells in human immunodeficiency virus-negative European peripheral pleomorphic T-cell lymphomas.
Blood. 1993 Jul 01; 82(1):217-23.Blood

Abstract

We investigated 81 cases of peripheral pleomorphic T-cell lymphoma (PMTCL) occurring in human immunodeficiency virus-negative Europeans for the presence of Epstein-Barr virus (EBV)-DNA through polymerase chain reaction (PCR) for the presence of EBV-encoded small nuclear RNAs (EBER) and immediate early mRNAs (Bam H-fragment, lower strand frame [BHLF]) by in situ hybridization (ISH) and for EBV-encoded latent membrane protein (LMP) and nuclear antigen 2 (EBNA2) by immunohistology (IH). EBER-ISH, which could be applied on all cases, showed an overall incidence of EBV-infected cells in 38 of 81 cases (47%) of PMTCL. These data could be confirmed by PCR, which produced congruent results in the cases with amplifiable DNA. By EBER-ISH, the virus was located in the tumor cells in 30 of the 38 EBV-positive cases, with the proportion of the infected cells ranging from 1% to 100%. In 18 of these cases and in the 8 cases without EBV-infected tumor cells, the virus was, respectively, either additionally or exclusively detectable in occasional nonmalignant lymphoid bystander cells. An LMP expression was observed in several of the EBER-expressing tumor cells in 18 cases, whereas EBNA2 was detectable only in one case, which also displayed signs of viral replication. Some nonmalignant EBV-infected B immunoblasts also expressed LMP in several cases. Primary cutaneous and enteropathy-associated PMTCL displayed less frequent EBV infection when compared with other extranodal or nodal manifestations.

Authors+Show Affiliations

Konsultations und Referenzzentrum für Lymphknotenund Hämatopathologie am Institut für Pathologie, Klinikum Steglitz, Freie Universität, Berlin, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8391874

Citation

Korbjuhn, P, et al. "Frequent Latent Epstein-Barr Virus Infection of Neoplastic T Cells and Bystander B Cells in Human Immunodeficiency Virus-negative European Peripheral Pleomorphic T-cell Lymphomas." Blood, vol. 82, no. 1, 1993, pp. 217-23.
Korbjuhn P, Anagnostopoulos I, Hummel M, et al. Frequent latent Epstein-Barr virus infection of neoplastic T cells and bystander B cells in human immunodeficiency virus-negative European peripheral pleomorphic T-cell lymphomas. Blood. 1993;82(1):217-23.
Korbjuhn, P., Anagnostopoulos, I., Hummel, M., Tiemann, M., Dallenbach, F., Parwaresch, M. R., & Stein, H. (1993). Frequent latent Epstein-Barr virus infection of neoplastic T cells and bystander B cells in human immunodeficiency virus-negative European peripheral pleomorphic T-cell lymphomas. Blood, 82(1), 217-23.
Korbjuhn P, et al. Frequent Latent Epstein-Barr Virus Infection of Neoplastic T Cells and Bystander B Cells in Human Immunodeficiency Virus-negative European Peripheral Pleomorphic T-cell Lymphomas. Blood. 1993 Jul 1;82(1):217-23. PubMed PMID: 8391874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequent latent Epstein-Barr virus infection of neoplastic T cells and bystander B cells in human immunodeficiency virus-negative European peripheral pleomorphic T-cell lymphomas. AU - Korbjuhn,P, AU - Anagnostopoulos,I, AU - Hummel,M, AU - Tiemann,M, AU - Dallenbach,F, AU - Parwaresch,M R, AU - Stein,H, PY - 1993/7/1/pubmed PY - 1993/7/1/medline PY - 1993/7/1/entrez SP - 217 EP - 23 JF - Blood JO - Blood VL - 82 IS - 1 N2 - We investigated 81 cases of peripheral pleomorphic T-cell lymphoma (PMTCL) occurring in human immunodeficiency virus-negative Europeans for the presence of Epstein-Barr virus (EBV)-DNA through polymerase chain reaction (PCR) for the presence of EBV-encoded small nuclear RNAs (EBER) and immediate early mRNAs (Bam H-fragment, lower strand frame [BHLF]) by in situ hybridization (ISH) and for EBV-encoded latent membrane protein (LMP) and nuclear antigen 2 (EBNA2) by immunohistology (IH). EBER-ISH, which could be applied on all cases, showed an overall incidence of EBV-infected cells in 38 of 81 cases (47%) of PMTCL. These data could be confirmed by PCR, which produced congruent results in the cases with amplifiable DNA. By EBER-ISH, the virus was located in the tumor cells in 30 of the 38 EBV-positive cases, with the proportion of the infected cells ranging from 1% to 100%. In 18 of these cases and in the 8 cases without EBV-infected tumor cells, the virus was, respectively, either additionally or exclusively detectable in occasional nonmalignant lymphoid bystander cells. An LMP expression was observed in several of the EBER-expressing tumor cells in 18 cases, whereas EBNA2 was detectable only in one case, which also displayed signs of viral replication. Some nonmalignant EBV-infected B immunoblasts also expressed LMP in several cases. Primary cutaneous and enteropathy-associated PMTCL displayed less frequent EBV infection when compared with other extranodal or nodal manifestations. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/8391874/Frequent_latent_Epstein_Barr_virus_infection_of_neoplastic_T_cells_and_bystander_B_cells_in_human_immunodeficiency_virus_negative_European_peripheral_pleomorphic_T_cell_lymphomas_ DB - PRIME DP - Unbound Medicine ER -