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Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae.
Eur J Biochem 1993; 216(3):849-61EJ

Abstract

Preparations of intact trehalose synthase contain three polypeptides with molecular masses of 56, 102 and 123 kDa. We have cloned the genes TSS1 and TSL1 coding for the 56- and 123-kDa subunits, respectively. These genes are located on chromosomes II (TSS1) and XIII (TSL1). The TSS1 gene was found to be identical with CIF1, a gene required for normal growth on glucose. The product of the entire TSS1 gene exhibits 37% identity with a 502-amino-acid stretch from the middle of the TSL1 product. Disruption of the TSS1 gene in yeast eliminates both trehalose 6-phosphate synthase (Tre6P synthase) and trehalose 6-phosphate phosphatase (Tre6Pase) activities, and reintroduction of this gene restores these activities. Transformation of Escherichia coli with TSS1 increases its Tre6P synthase activity. Specific proteolytic degradation of the 123-kDa polypeptide from the N-terminus greatly influences the Tre6P synthase activity, decreasing its inhibition by phosphate and activatability by fructose 6-phosphate but has little effect on the Tre6Pase activity. These results suggest that this N-terminal part confers regulatory properties upon the Tre6P synthase activity.

Authors+Show Affiliations

Research Laboratory, Alko Ltd, Helsinki, Finland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8404905

Citation

Vuorio, O E., et al. "Cloning of Two Related Genes Encoding the 56-kDa and 123-kDa Subunits of Trehalose Synthase From the Yeast Saccharomyces Cerevisiae." European Journal of Biochemistry, vol. 216, no. 3, 1993, pp. 849-61.
Vuorio OE, Kalkkinen N, Londesborough J. Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae. Eur J Biochem. 1993;216(3):849-61.
Vuorio, O. E., Kalkkinen, N., & Londesborough, J. (1993). Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae. European Journal of Biochemistry, 216(3), pp. 849-61.
Vuorio OE, Kalkkinen N, Londesborough J. Cloning of Two Related Genes Encoding the 56-kDa and 123-kDa Subunits of Trehalose Synthase From the Yeast Saccharomyces Cerevisiae. Eur J Biochem. 1993 Sep 15;216(3):849-61. PubMed PMID: 8404905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae. AU - Vuorio,O E, AU - Kalkkinen,N, AU - Londesborough,J, PY - 1993/9/15/pubmed PY - 1993/9/15/medline PY - 1993/9/15/entrez SP - 849 EP - 61 JF - European journal of biochemistry JO - Eur. J. Biochem. VL - 216 IS - 3 N2 - Preparations of intact trehalose synthase contain three polypeptides with molecular masses of 56, 102 and 123 kDa. We have cloned the genes TSS1 and TSL1 coding for the 56- and 123-kDa subunits, respectively. These genes are located on chromosomes II (TSS1) and XIII (TSL1). The TSS1 gene was found to be identical with CIF1, a gene required for normal growth on glucose. The product of the entire TSS1 gene exhibits 37% identity with a 502-amino-acid stretch from the middle of the TSL1 product. Disruption of the TSS1 gene in yeast eliminates both trehalose 6-phosphate synthase (Tre6P synthase) and trehalose 6-phosphate phosphatase (Tre6Pase) activities, and reintroduction of this gene restores these activities. Transformation of Escherichia coli with TSS1 increases its Tre6P synthase activity. Specific proteolytic degradation of the 123-kDa polypeptide from the N-terminus greatly influences the Tre6P synthase activity, decreasing its inhibition by phosphate and activatability by fructose 6-phosphate but has little effect on the Tre6Pase activity. These results suggest that this N-terminal part confers regulatory properties upon the Tre6P synthase activity. SN - 0014-2956 UR - https://www.unboundmedicine.com/medline/citation/8404905/Cloning_of_two_related_genes_encoding_the_56_kDa_and_123_kDa_subunits_of_trehalose_synthase_from_the_yeast_Saccharomyces_cerevisiae_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2956&date=1993&volume=216&issue=3&spage=849 DB - PRIME DP - Unbound Medicine ER -