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Molecular cloning and characterization of acrA and acrE genes of Escherichia coli.
J Bacteriol. 1993 Oct; 175(19):6299-313.JB

Abstract

The DNA fragment containing the acrA locus of the Escherichia coli chromosome has been cloned by using a complementation test. The nucleotide sequence indicates the presence of two open reading frames (ORFs). Sequence analysis suggests that the first ORF encodes a 397-residue lipoprotein with a 24-amino-acid signal peptide at its N terminus. One inactive allele of acrA from strain N43 was shown to contain an IS2 element inserted into this ORF. Therefore, this ORF was designated acrA. The second downstream ORF is predicted to encode a transmembrane protein of 1,049 amino acids and is named acrE. Genes acrA and acrE are probably located on the same operon, and both of their products are likely to affect drug susceptibilities observed in wild-type cells. The cellular localizations of these polypeptides have been analyzed by making acrA::TnphoA and acrE::TnphoA fusion proteins. Interestingly, AcrA and AcrE share 65 and 77% amino acid identity with two other E. coli polypeptides, EnvC and EnvD, respectively. Drug susceptibilities in one acrA mutant (N43) and one envCD mutant (PM61) have been determined and compared. Finally, the possible functions of these proteins are discussed.

Authors+Show Affiliations

Department of Chemistry, University of California, Berkeley.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8407802

Citation

Ma, D, et al. "Molecular Cloning and Characterization of acrA and acrE Genes of Escherichia Coli." Journal of Bacteriology, vol. 175, no. 19, 1993, pp. 6299-313.
Ma D, Cook DN, Alberti M, et al. Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J Bacteriol. 1993;175(19):6299-313.
Ma, D., Cook, D. N., Alberti, M., Pon, N. G., Nikaido, H., & Hearst, J. E. (1993). Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. Journal of Bacteriology, 175(19), 6299-313.
Ma D, et al. Molecular Cloning and Characterization of acrA and acrE Genes of Escherichia Coli. J Bacteriol. 1993;175(19):6299-313. PubMed PMID: 8407802.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. AU - Ma,D, AU - Cook,D N, AU - Alberti,M, AU - Pon,N G, AU - Nikaido,H, AU - Hearst,J E, PY - 1993/10/1/pubmed PY - 1993/10/1/medline PY - 1993/10/1/entrez SP - 6299 EP - 313 JF - Journal of bacteriology JO - J Bacteriol VL - 175 IS - 19 N2 - The DNA fragment containing the acrA locus of the Escherichia coli chromosome has been cloned by using a complementation test. The nucleotide sequence indicates the presence of two open reading frames (ORFs). Sequence analysis suggests that the first ORF encodes a 397-residue lipoprotein with a 24-amino-acid signal peptide at its N terminus. One inactive allele of acrA from strain N43 was shown to contain an IS2 element inserted into this ORF. Therefore, this ORF was designated acrA. The second downstream ORF is predicted to encode a transmembrane protein of 1,049 amino acids and is named acrE. Genes acrA and acrE are probably located on the same operon, and both of their products are likely to affect drug susceptibilities observed in wild-type cells. The cellular localizations of these polypeptides have been analyzed by making acrA::TnphoA and acrE::TnphoA fusion proteins. Interestingly, AcrA and AcrE share 65 and 77% amino acid identity with two other E. coli polypeptides, EnvC and EnvD, respectively. Drug susceptibilities in one acrA mutant (N43) and one envCD mutant (PM61) have been determined and compared. Finally, the possible functions of these proteins are discussed. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/8407802/Molecular_cloning_and_characterization_of_acrA_and_acrE_genes_of_Escherichia_coli_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=8407802 DB - PRIME DP - Unbound Medicine ER -