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Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase.
J Gen Microbiol. 1993 Aug; 139(8):1795-805.JG

Abstract

A mutant of Escherichia coli has been isolated that is unable to grow aerobically on non-fermentable substrates, but able to grow anaerobically on glycerol with alternative electron acceptors such as fumarate. Nitrate as electron acceptor supports anaerobic growth on glycerol, but not on succinate or lactate. Oxygen consumption rates by cell-free extracts with succinate, lactate or glycerol 3-phosphate as substrates were low relative to activities in an isogenic control strain but were restored in vitro by adding ubiquinone-1. Transformation of the mutant with a cloned 2.6 kb ClaI-PvuII fragment of chromosomal DNA restored cellular quinone levels and growth on succinate. The plasmid also complemented a previously isolated ubiA mutant for aerobic growth on non-fermentable substrates. The nucleotide sequence of the cloned fragment revealed a fragment of plsB (91.7 min on the E. coli chromosome map) and three open reading frames (ORFs), one of which (ORF3) encodes a protein with a predicted molecular mass of 32511 Da. The hydrophobicity profile of the ORF3 protein is characteristic of a membrane protein with five hydrophobic regions and is very similar to that of the Saccharomyces cerevisiae COQ2 gene product (p-hydroxybenzoate:polyprenyltransferase, required for the second step of ubiquinone biosynthesis) and to the product of the E. coli cyoE gene. Complementation of ubi mutants with various deletion derivatives of the cloned DNA fragment confirms that ORF3 is ubiA. ORF3 is closely linked to ubiC (ORF2), which encodes chorismate lyase.

Authors+Show Affiliations

Microbial Physiology Research Group, Division of Life Sciences, King's College, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8409922

Citation

Wu, G, et al. "Mutants of Escherichia Coli Affected in Respiration: the Cloning and Nucleotide Sequence of ubiA, Encoding the Membrane-bound P-hydroxybenzoate:octaprenyltransferase." Journal of General Microbiology, vol. 139, no. 8, 1993, pp. 1795-805.
Wu G, Williams HD, Gibson F, et al. Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase. J Gen Microbiol. 1993;139(8):1795-805.
Wu, G., Williams, H. D., Gibson, F., & Poole, R. K. (1993). Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase. Journal of General Microbiology, 139(8), 1795-805.
Wu G, et al. Mutants of Escherichia Coli Affected in Respiration: the Cloning and Nucleotide Sequence of ubiA, Encoding the Membrane-bound P-hydroxybenzoate:octaprenyltransferase. J Gen Microbiol. 1993;139(8):1795-805. PubMed PMID: 8409922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase. AU - Wu,G, AU - Williams,H D, AU - Gibson,F, AU - Poole,R K, PY - 1993/8/1/pubmed PY - 1993/8/1/medline PY - 1993/8/1/entrez SP - 1795 EP - 805 JF - Journal of general microbiology JO - J Gen Microbiol VL - 139 IS - 8 N2 - A mutant of Escherichia coli has been isolated that is unable to grow aerobically on non-fermentable substrates, but able to grow anaerobically on glycerol with alternative electron acceptors such as fumarate. Nitrate as electron acceptor supports anaerobic growth on glycerol, but not on succinate or lactate. Oxygen consumption rates by cell-free extracts with succinate, lactate or glycerol 3-phosphate as substrates were low relative to activities in an isogenic control strain but were restored in vitro by adding ubiquinone-1. Transformation of the mutant with a cloned 2.6 kb ClaI-PvuII fragment of chromosomal DNA restored cellular quinone levels and growth on succinate. The plasmid also complemented a previously isolated ubiA mutant for aerobic growth on non-fermentable substrates. The nucleotide sequence of the cloned fragment revealed a fragment of plsB (91.7 min on the E. coli chromosome map) and three open reading frames (ORFs), one of which (ORF3) encodes a protein with a predicted molecular mass of 32511 Da. The hydrophobicity profile of the ORF3 protein is characteristic of a membrane protein with five hydrophobic regions and is very similar to that of the Saccharomyces cerevisiae COQ2 gene product (p-hydroxybenzoate:polyprenyltransferase, required for the second step of ubiquinone biosynthesis) and to the product of the E. coli cyoE gene. Complementation of ubi mutants with various deletion derivatives of the cloned DNA fragment confirms that ORF3 is ubiA. ORF3 is closely linked to ubiC (ORF2), which encodes chorismate lyase. SN - 0022-1287 UR - https://www.unboundmedicine.com/medline/citation/8409922/Mutants_of_Escherichia_coli_affected_in_respiration:_the_cloning_and_nucleotide_sequence_of_ubiA_encoding_the_membrane_bound_p_hydroxybenzoate:octaprenyltransferase_ L2 - https://ecocyc.org/gene?orgid=ECOLI&id=EG11370 DB - PRIME DP - Unbound Medicine ER -