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Genistein exhibits preferential cytotoxicity to a leukemogenic variant but induces differentiation of a non-leukemogenic variant of the mouse monocytic leukemia Mm cell line.
Leuk Res. 1993 Oct; 17(10):847-53.LR

Abstract

Mouse leukemia Mm-A and Mm-S2 cells are subclones of mouse monocytic leukemia Mm cells, Mm-A cells having much higher leukemogenicity than Mm-S2 cells. The growth-inhibitory effects of several protein kinase inhibitors on leukemogenic Mm-A and non-leukemogenic Mm-S2 cells were examined. Most inhibitors of protein serine/threonine kinases inhibited the growth of Mm-A and Mm-S2 cells similarly, but some protein tyrosine kinase inhibitors exhibited differential inhibitory effects on Mm-A and Mm-S2 cells. Genistein inhibited growth of Mm-A cells more effectively than that of Mm-S2 cells, but another inhibitor of tyrosine kinase, herbimycin A, preferentially inhibited growth of non-leukemogenic Mm-S2 cells. Genistein induced or enhanced several differentiation markers of Mm-S2 cells, such as cell spreading, immunophagocytosis, nitroblue tetrazolium (NBT) reduction and lysozyme activity in a dose-dependent manner, but herbimycin A did not. Genistein was cytotoxic to Mm-A cells rather than inducing cell differentiation. Genistein has effects on several other cellular events as well as inhibition of tyrosine kinases. However, it effectively inhibited protein tyrosine phosphorylation in Mm-A cells and its decrease of tyrosine phosphorylation was closely associated with its inhibition of cell growth. Thus, a genistein-sensitive tyrosine kinase(s) may play an important role in the growth and/or survival of leukemogenic Mm-A cells.

Authors+Show Affiliations

Saitama Cancer Center Research Institute, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8412297

Citation

Kanatani, Y, et al. "Genistein Exhibits Preferential Cytotoxicity to a Leukemogenic Variant but Induces Differentiation of a Non-leukemogenic Variant of the Mouse Monocytic Leukemia Mm Cell Line." Leukemia Research, vol. 17, no. 10, 1993, pp. 847-53.
Kanatani Y, Kasukabe T, Hozumi M, et al. Genistein exhibits preferential cytotoxicity to a leukemogenic variant but induces differentiation of a non-leukemogenic variant of the mouse monocytic leukemia Mm cell line. Leuk Res. 1993;17(10):847-53.
Kanatani, Y., Kasukabe, T., Hozumi, M., Motoyoshi, K., Nagata, N., & Honma, Y. (1993). Genistein exhibits preferential cytotoxicity to a leukemogenic variant but induces differentiation of a non-leukemogenic variant of the mouse monocytic leukemia Mm cell line. Leukemia Research, 17(10), 847-53.
Kanatani Y, et al. Genistein Exhibits Preferential Cytotoxicity to a Leukemogenic Variant but Induces Differentiation of a Non-leukemogenic Variant of the Mouse Monocytic Leukemia Mm Cell Line. Leuk Res. 1993;17(10):847-53. PubMed PMID: 8412297.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genistein exhibits preferential cytotoxicity to a leukemogenic variant but induces differentiation of a non-leukemogenic variant of the mouse monocytic leukemia Mm cell line. AU - Kanatani,Y, AU - Kasukabe,T, AU - Hozumi,M, AU - Motoyoshi,K, AU - Nagata,N, AU - Honma,Y, PY - 1993/10/1/pubmed PY - 1993/10/1/medline PY - 1993/10/1/entrez SP - 847 EP - 53 JF - Leukemia research JO - Leuk Res VL - 17 IS - 10 N2 - Mouse leukemia Mm-A and Mm-S2 cells are subclones of mouse monocytic leukemia Mm cells, Mm-A cells having much higher leukemogenicity than Mm-S2 cells. The growth-inhibitory effects of several protein kinase inhibitors on leukemogenic Mm-A and non-leukemogenic Mm-S2 cells were examined. Most inhibitors of protein serine/threonine kinases inhibited the growth of Mm-A and Mm-S2 cells similarly, but some protein tyrosine kinase inhibitors exhibited differential inhibitory effects on Mm-A and Mm-S2 cells. Genistein inhibited growth of Mm-A cells more effectively than that of Mm-S2 cells, but another inhibitor of tyrosine kinase, herbimycin A, preferentially inhibited growth of non-leukemogenic Mm-S2 cells. Genistein induced or enhanced several differentiation markers of Mm-S2 cells, such as cell spreading, immunophagocytosis, nitroblue tetrazolium (NBT) reduction and lysozyme activity in a dose-dependent manner, but herbimycin A did not. Genistein was cytotoxic to Mm-A cells rather than inducing cell differentiation. Genistein has effects on several other cellular events as well as inhibition of tyrosine kinases. However, it effectively inhibited protein tyrosine phosphorylation in Mm-A cells and its decrease of tyrosine phosphorylation was closely associated with its inhibition of cell growth. Thus, a genistein-sensitive tyrosine kinase(s) may play an important role in the growth and/or survival of leukemogenic Mm-A cells. SN - 0145-2126 UR - https://www.unboundmedicine.com/medline/citation/8412297/Genistein_exhibits_preferential_cytotoxicity_to_a_leukemogenic_variant_but_induces_differentiation_of_a_non_leukemogenic_variant_of_the_mouse_monocytic_leukemia_Mm_cell_line_ DB - PRIME DP - Unbound Medicine ER -