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Contrasting effect of transforming growth factor type beta 1 (TGF-beta 1) on proliferation and interleukin-2 receptor expression in activated and rapidly cycling immature (CD3-CD4-CD8-) thymocytes.
J Cell Physiol. 1993 Jan; 154(1):44-52.JC

Abstract

Transforming growth factor beta (TGF-beta) is a cytokine with immunoregulatory properties that acts negatively on T lymphocyte proliferation. However, with the EL 4-6.1 variant of the murine thymoma EL 4 activated with phorbol ester and/or interleukin-1 (IL-1), we recently found that it up-regulates interleukin-2-receptor (IL-2R) expression. Since EL 4-6.1 cells share phenotypic and functional characteristics with the immature thymic subset lacking CD4 and CD8 accessory molecules (DN), we investigated the effect of TGF-beta 1 on the IL-2R 55kD alpha chain expression and proliferation of activated DN cells and especially in DN cells that do not express CD3. We observed that TGF-beta 1 was able to increase both the percentage of CD3-DN cells expressing IL-2R alpha chains and the expression of IL-2R alpha chain in these cells. This stimulatory effect of TGF-beta 1 was distal from early transduction events. In addition, TGF-beta 1 was found to modulate CD3-DN cell proliferation. During differentiation in the thymus, CD3-DN cells transiently express the IL-2R alpha chain of the IL-2R and these IL-2R+ CD3-DN cells are preprogrammed to down-regulate the IL-2R alpha chain and up-regulate the CD4 and CD8 accessory molecule. We thus also tested the effect of TGF-beta 1 on IL-2R alpha chain expression in these in vitro differentiating CD3-DN cells. We found that TGF-beta 1 neither significantly affected IL-2R expression nor changed CD4 or CD8 expression. Hence, in CD3-DN cells, the effect of TGF-beta 1 on IL-2R expression seems to be restricted to proliferating cells.

Authors+Show Affiliations

Unité INSERM 291, Montpellier, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8419406

Citation

Dupuy d'Angeac, A, et al. "Contrasting Effect of Transforming Growth Factor Type Beta 1 (TGF-beta 1) On Proliferation and Interleukin-2 Receptor Expression in Activated and Rapidly Cycling Immature (CD3-CD4-CD8-) Thymocytes." Journal of Cellular Physiology, vol. 154, no. 1, 1993, pp. 44-52.
Dupuy d'Angeac A, Rème T, Monier S, et al. Contrasting effect of transforming growth factor type beta 1 (TGF-beta 1) on proliferation and interleukin-2 receptor expression in activated and rapidly cycling immature (CD3-CD4-CD8-) thymocytes. J Cell Physiol. 1993;154(1):44-52.
Dupuy d'Angeac, A., Rème, T., Monier, S., Gao, Q., Duperray, C., Jullien, P., & Dornand, J. (1993). Contrasting effect of transforming growth factor type beta 1 (TGF-beta 1) on proliferation and interleukin-2 receptor expression in activated and rapidly cycling immature (CD3-CD4-CD8-) thymocytes. Journal of Cellular Physiology, 154(1), 44-52.
Dupuy d'Angeac A, et al. Contrasting Effect of Transforming Growth Factor Type Beta 1 (TGF-beta 1) On Proliferation and Interleukin-2 Receptor Expression in Activated and Rapidly Cycling Immature (CD3-CD4-CD8-) Thymocytes. J Cell Physiol. 1993;154(1):44-52. PubMed PMID: 8419406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contrasting effect of transforming growth factor type beta 1 (TGF-beta 1) on proliferation and interleukin-2 receptor expression in activated and rapidly cycling immature (CD3-CD4-CD8-) thymocytes. AU - Dupuy d'Angeac,A, AU - Rème,T, AU - Monier,S, AU - Gao,Q, AU - Duperray,C, AU - Jullien,P, AU - Dornand,J, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 44 EP - 52 JF - Journal of cellular physiology JO - J Cell Physiol VL - 154 IS - 1 N2 - Transforming growth factor beta (TGF-beta) is a cytokine with immunoregulatory properties that acts negatively on T lymphocyte proliferation. However, with the EL 4-6.1 variant of the murine thymoma EL 4 activated with phorbol ester and/or interleukin-1 (IL-1), we recently found that it up-regulates interleukin-2-receptor (IL-2R) expression. Since EL 4-6.1 cells share phenotypic and functional characteristics with the immature thymic subset lacking CD4 and CD8 accessory molecules (DN), we investigated the effect of TGF-beta 1 on the IL-2R 55kD alpha chain expression and proliferation of activated DN cells and especially in DN cells that do not express CD3. We observed that TGF-beta 1 was able to increase both the percentage of CD3-DN cells expressing IL-2R alpha chains and the expression of IL-2R alpha chain in these cells. This stimulatory effect of TGF-beta 1 was distal from early transduction events. In addition, TGF-beta 1 was found to modulate CD3-DN cell proliferation. During differentiation in the thymus, CD3-DN cells transiently express the IL-2R alpha chain of the IL-2R and these IL-2R+ CD3-DN cells are preprogrammed to down-regulate the IL-2R alpha chain and up-regulate the CD4 and CD8 accessory molecule. We thus also tested the effect of TGF-beta 1 on IL-2R alpha chain expression in these in vitro differentiating CD3-DN cells. We found that TGF-beta 1 neither significantly affected IL-2R expression nor changed CD4 or CD8 expression. Hence, in CD3-DN cells, the effect of TGF-beta 1 on IL-2R expression seems to be restricted to proliferating cells. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/8419406/Contrasting_effect_of_transforming_growth_factor_type_beta_1__TGF_beta_1__on_proliferation_and_interleukin_2_receptor_expression_in_activated_and_rapidly_cycling_immature__CD3_CD4_CD8___thymocytes_ L2 - https://doi.org/10.1002/jcp.1041540107 DB - PRIME DP - Unbound Medicine ER -