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Role of tumor necrosis factor alpha in activation and replication of the tat-defective human immunodeficiency virus type 1.
J Virol. 1993 Feb; 67(2):1094-9.JV

Abstract

Transcription of human immunodeficiency virus type 1 (HIV-1) depends on the function of the virus-encoded regulatory protein Tat, which interacts with the specific Tat response (TAR) element present in the leader sequence of all HIV-1 RNAs. In this study, we examined whether tumor necrosis factor alpha (TNF-alpha) can replace the requirement for a functional Tat protein. We found that TNF-alpha can induce expression of a latent, tat-defective virus and support its replication both in T cells and in primary mononuclear cells. Analysis of the transcriptional rate of the tat-defective HIV-1 transcriptional unit indicates that TNF-alpha stimulates the initiation of transcription but, in contrast to Tat protein, does not significantly reduce transcriptional polarity. Interestingly, we found that the processing of viral precursor proteins is altered in the absence of Tat. We propose that TNF-alpha-mediated induction of HIV-1 plays an essential role in the early stages of the virus life cycle and in viral latency.

Authors+Show Affiliations

Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8419639

Citation

Popik, W, and P M. Pitha. "Role of Tumor Necrosis Factor Alpha in Activation and Replication of the Tat-defective Human Immunodeficiency Virus Type 1." Journal of Virology, vol. 67, no. 2, 1993, pp. 1094-9.
Popik W, Pitha PM. Role of tumor necrosis factor alpha in activation and replication of the tat-defective human immunodeficiency virus type 1. J Virol. 1993;67(2):1094-9.
Popik, W., & Pitha, P. M. (1993). Role of tumor necrosis factor alpha in activation and replication of the tat-defective human immunodeficiency virus type 1. Journal of Virology, 67(2), 1094-9.
Popik W, Pitha PM. Role of Tumor Necrosis Factor Alpha in Activation and Replication of the Tat-defective Human Immunodeficiency Virus Type 1. J Virol. 1993;67(2):1094-9. PubMed PMID: 8419639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of tumor necrosis factor alpha in activation and replication of the tat-defective human immunodeficiency virus type 1. AU - Popik,W, AU - Pitha,P M, PY - 1993/2/1/pubmed PY - 1993/2/1/medline PY - 1993/2/1/entrez SP - 1094 EP - 9 JF - Journal of virology JO - J Virol VL - 67 IS - 2 N2 - Transcription of human immunodeficiency virus type 1 (HIV-1) depends on the function of the virus-encoded regulatory protein Tat, which interacts with the specific Tat response (TAR) element present in the leader sequence of all HIV-1 RNAs. In this study, we examined whether tumor necrosis factor alpha (TNF-alpha) can replace the requirement for a functional Tat protein. We found that TNF-alpha can induce expression of a latent, tat-defective virus and support its replication both in T cells and in primary mononuclear cells. Analysis of the transcriptional rate of the tat-defective HIV-1 transcriptional unit indicates that TNF-alpha stimulates the initiation of transcription but, in contrast to Tat protein, does not significantly reduce transcriptional polarity. Interestingly, we found that the processing of viral precursor proteins is altered in the absence of Tat. We propose that TNF-alpha-mediated induction of HIV-1 plays an essential role in the early stages of the virus life cycle and in viral latency. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/8419639/Role_of_tumor_necrosis_factor_alpha_in_activation_and_replication_of_the_tat_defective_human_immunodeficiency_virus_type_1_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=8419639 DB - PRIME DP - Unbound Medicine ER -