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Possible morphological substrates for GABA-mediated presynaptic inhibition in the lamprey spinal cord.
J Comp Neurol. 1993 Feb 22; 328(4):463-72.JC

Abstract

Gamma-aminobutyric acid (GABA) neurons intrinsic to the lamprey spinal cord are known to modulate synaptic transmission from interneurons active during locomotion and from mechanosensory dorsal cells. Many of these physiological effects are presynaptic. To establish the morphological substrates for these axo-axonic interactions, an ultrastructural analysis was performed with an antiserum to fixed GABA. The GABA immunoreactivity (ir) was detected by postembedding peroxidase-antiperoxidase and immunogold techniques. GABA-ir terminals were found to make appositions with unlabelled axons located in the dorsal columns and in the ventrolateral aspect of the spinal cord. In the ventrolateral part of the cord, similar appositions between different GABA-ir terminals were also observed. The immunolabelled terminals contained spherical to pleomorphic synaptic vesicles, and also glycogen granules and dense core vesicles. In some cases, the fine structure of the contacts between immunogold-labelled terminals and unlabelled axons suggested a synaptic relationship. Such a relation was found in a relatively small proportion (2-3%) of the appositions studied. These specializations were always observed in close relation to an output synapse of the postsynaptic axon. It is suggested that the axo-axonal contacts described may provide an effective modulation of the synaptic transmission from axons in the lamprey spinal cord.

Authors+Show Affiliations

Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8429129

Citation

Christenson, J, et al. "Possible Morphological Substrates for GABA-mediated Presynaptic Inhibition in the Lamprey Spinal Cord." The Journal of Comparative Neurology, vol. 328, no. 4, 1993, pp. 463-72.
Christenson J, Shupliakov O, Cullheim S, et al. Possible morphological substrates for GABA-mediated presynaptic inhibition in the lamprey spinal cord. J Comp Neurol. 1993;328(4):463-72.
Christenson, J., Shupliakov, O., Cullheim, S., & Grillner, S. (1993). Possible morphological substrates for GABA-mediated presynaptic inhibition in the lamprey spinal cord. The Journal of Comparative Neurology, 328(4), 463-72.
Christenson J, et al. Possible Morphological Substrates for GABA-mediated Presynaptic Inhibition in the Lamprey Spinal Cord. J Comp Neurol. 1993 Feb 22;328(4):463-72. PubMed PMID: 8429129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible morphological substrates for GABA-mediated presynaptic inhibition in the lamprey spinal cord. AU - Christenson,J, AU - Shupliakov,O, AU - Cullheim,S, AU - Grillner,S, PY - 1993/2/22/pubmed PY - 1993/2/22/medline PY - 1993/2/22/entrez SP - 463 EP - 72 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 328 IS - 4 N2 - Gamma-aminobutyric acid (GABA) neurons intrinsic to the lamprey spinal cord are known to modulate synaptic transmission from interneurons active during locomotion and from mechanosensory dorsal cells. Many of these physiological effects are presynaptic. To establish the morphological substrates for these axo-axonic interactions, an ultrastructural analysis was performed with an antiserum to fixed GABA. The GABA immunoreactivity (ir) was detected by postembedding peroxidase-antiperoxidase and immunogold techniques. GABA-ir terminals were found to make appositions with unlabelled axons located in the dorsal columns and in the ventrolateral aspect of the spinal cord. In the ventrolateral part of the cord, similar appositions between different GABA-ir terminals were also observed. The immunolabelled terminals contained spherical to pleomorphic synaptic vesicles, and also glycogen granules and dense core vesicles. In some cases, the fine structure of the contacts between immunogold-labelled terminals and unlabelled axons suggested a synaptic relationship. Such a relation was found in a relatively small proportion (2-3%) of the appositions studied. These specializations were always observed in close relation to an output synapse of the postsynaptic axon. It is suggested that the axo-axonal contacts described may provide an effective modulation of the synaptic transmission from axons in the lamprey spinal cord. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/8429129/Possible_morphological_substrates_for_GABA_mediated_presynaptic_inhibition_in_the_lamprey_spinal_cord_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1993&volume=328&issue=4&spage=463 DB - PRIME DP - Unbound Medicine ER -