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Evaluation of an immunoblot assay for serological confirmation and differentiation of human T-cell lymphotropic virus types I and II.
J Clin Microbiol. 1993 Feb; 31(2):260-4.JC

Abstract

The confirmation of infection with human T-cell lymphotropic virus type I (HTLV-I) and type II (HTLV-II) currently involves multiple assays. These include Western blot (immunoblot) (WB) and/or radioimmunoprecipitation assay for detection of antibodies to HTLV-specific viral proteins and polymerase chain reaction and/or peptide-based enzyme immunoassays for differentiating between the two viruses. We undertook an evaluation of a modified WB assay that includes native HTLV-I viral proteins from MT-2 cells spiked with an HTLV-I transmembrane glycoprotein (recombinant p21e) and the HTLV-I- and HTLV-II-specific recombinant proteins MTA-1 and K55. The test panel consisted of well-characterized sera from U.S. blood donors, American Indians, intravenous drug users, and patients seen in sexually transmitted disease clinics. Of 158 HTLV-I/II-seropositive serum specimens tested, 156 (98.7%) were confirmed and typed as HTLV-I or HTLV-II. Of 82 HTLV-I/II-seroindeterminate or -seronegative serum specimens, only 1 was classified as HTLV-II positive: the sample had weak gag p19 and strong gag p24 and env p21e reactivity and was radioimmunoprecipitation assay negative for env gp61/68 but polymerase chain reaction positive for HTLV-II. The specificity of the modified WB for confirming and typing serum samples was therefore 100%. We conclude that this WB assay is useful for confirming and typing HTLV infection and can help simplify HTLV-I/II testing algorithms.

Authors+Show Affiliations

Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8432811

Citation

Roberts, B D., et al. "Evaluation of an Immunoblot Assay for Serological Confirmation and Differentiation of Human T-cell Lymphotropic Virus Types I and II." Journal of Clinical Microbiology, vol. 31, no. 2, 1993, pp. 260-4.
Roberts BD, Foung SK, Lipka JJ, et al. Evaluation of an immunoblot assay for serological confirmation and differentiation of human T-cell lymphotropic virus types I and II. J Clin Microbiol. 1993;31(2):260-4.
Roberts, B. D., Foung, S. K., Lipka, J. J., Kaplan, J. E., Hadlock, K. G., Reyes, G. R., Chan, L., Heneine, W., & Khabbaz, R. F. (1993). Evaluation of an immunoblot assay for serological confirmation and differentiation of human T-cell lymphotropic virus types I and II. Journal of Clinical Microbiology, 31(2), 260-4.
Roberts BD, et al. Evaluation of an Immunoblot Assay for Serological Confirmation and Differentiation of Human T-cell Lymphotropic Virus Types I and II. J Clin Microbiol. 1993;31(2):260-4. PubMed PMID: 8432811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of an immunoblot assay for serological confirmation and differentiation of human T-cell lymphotropic virus types I and II. AU - Roberts,B D, AU - Foung,S K, AU - Lipka,J J, AU - Kaplan,J E, AU - Hadlock,K G, AU - Reyes,G R, AU - Chan,L, AU - Heneine,W, AU - Khabbaz,R F, PY - 1993/2/1/pubmed PY - 1993/2/1/medline PY - 1993/2/1/entrez SP - 260 EP - 4 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 31 IS - 2 N2 - The confirmation of infection with human T-cell lymphotropic virus type I (HTLV-I) and type II (HTLV-II) currently involves multiple assays. These include Western blot (immunoblot) (WB) and/or radioimmunoprecipitation assay for detection of antibodies to HTLV-specific viral proteins and polymerase chain reaction and/or peptide-based enzyme immunoassays for differentiating between the two viruses. We undertook an evaluation of a modified WB assay that includes native HTLV-I viral proteins from MT-2 cells spiked with an HTLV-I transmembrane glycoprotein (recombinant p21e) and the HTLV-I- and HTLV-II-specific recombinant proteins MTA-1 and K55. The test panel consisted of well-characterized sera from U.S. blood donors, American Indians, intravenous drug users, and patients seen in sexually transmitted disease clinics. Of 158 HTLV-I/II-seropositive serum specimens tested, 156 (98.7%) were confirmed and typed as HTLV-I or HTLV-II. Of 82 HTLV-I/II-seroindeterminate or -seronegative serum specimens, only 1 was classified as HTLV-II positive: the sample had weak gag p19 and strong gag p24 and env p21e reactivity and was radioimmunoprecipitation assay negative for env gp61/68 but polymerase chain reaction positive for HTLV-II. The specificity of the modified WB for confirming and typing serum samples was therefore 100%. We conclude that this WB assay is useful for confirming and typing HTLV infection and can help simplify HTLV-I/II testing algorithms. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/8432811/Evaluation_of_an_immunoblot_assay_for_serological_confirmation_and_differentiation_of_human_T_cell_lymphotropic_virus_types_I_and_II_ L2 - https://journals.asm.org/doi/10.1128/jcm.31.2.260-264.1993?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -