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Glutamine enhancement of structure and function in transplanted small intestine in the rat.
JPEN J Parenter Enteral Nutr. 1993 Jan-Feb; 17(1):47-55.JJ

Abstract

Total parenteral nutrition is required by all patients in need of small bowel transplantation. Untoward side effects of total parenteral nutrition include atrophy and hypofunction of the small intestine. Glutamine, the preferred fuel for the enterocyte, is presumably present in insufficient amounts in diets given to patients with intestinal dysfunction. In a rat model of total parenteral nutrition and small bowel transplantation, this study investigated the following: (1) whether glutamine improves graft structure and function, (2) the optimal route of glutamine delivery (intravenous vs direct infusion into the graft), and (3) the effect of glutamine on ultrastructure of the graft enterocyte. Lewis rats underwent small bowel transplantation as a Thiry-Vella graft and received total parenteral nutrition for 14 days while assigned to one of four infusion groups: 2% intravenous glutamine; 2% intravenous isonitrogenous mixture, nonessential amino acids (control); 2% glutamine into the graft; or 2% nonessential amino acids into the graft (control). Graft mucosal villous height, villous surface area, crypt depth, weight, protein, deoxyribonucleic acid content, glucose absorption, and enterocyte ultrastructure were then evaluated. Infusion of glutamine directly into the graft significantly increased mucosal villous height (p = .045), surface area (p = .029), and glucose absorption (p = .004) when compared with controls. Intravenous glutamine infusion significantly increased mucosal villous height (p = .002), surface area (p = .001), weight (p = .005), and glucose absorption (p = .04) when compared with controls. Most enterotrophic and functional benefits of glutamine were not significantly different between intravenous infusions and direct administration into the graft.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Harrison Department of Surgical Research, Hospital of the University of Pennsylvania, Wistar Institute, Philadelphia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8437324

Citation

Frankel, W L., et al. "Glutamine Enhancement of Structure and Function in Transplanted Small Intestine in the Rat." JPEN. Journal of Parenteral and Enteral Nutrition, vol. 17, no. 1, 1993, pp. 47-55.
Frankel WL, Zhang W, Afonso J, et al. Glutamine enhancement of structure and function in transplanted small intestine in the rat. JPEN J Parenter Enteral Nutr. 1993;17(1):47-55.
Frankel, W. L., Zhang, W., Afonso, J., Klurfeld, D. M., Don, S. H., Laitin, E., Deaton, D., Furth, E. E., Pietra, G. G., & Naji, A. (1993). Glutamine enhancement of structure and function in transplanted small intestine in the rat. JPEN. Journal of Parenteral and Enteral Nutrition, 17(1), 47-55.
Frankel WL, et al. Glutamine Enhancement of Structure and Function in Transplanted Small Intestine in the Rat. JPEN J Parenter Enteral Nutr. 1993 Jan-Feb;17(1):47-55. PubMed PMID: 8437324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutamine enhancement of structure and function in transplanted small intestine in the rat. A1 - Frankel,W L, AU - Zhang,W, AU - Afonso,J, AU - Klurfeld,D M, AU - Don,S H, AU - Laitin,E, AU - Deaton,D, AU - Furth,E E, AU - Pietra,G G, AU - Naji,A, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 47 EP - 55 JF - JPEN. Journal of parenteral and enteral nutrition JO - JPEN J Parenter Enteral Nutr VL - 17 IS - 1 N2 - Total parenteral nutrition is required by all patients in need of small bowel transplantation. Untoward side effects of total parenteral nutrition include atrophy and hypofunction of the small intestine. Glutamine, the preferred fuel for the enterocyte, is presumably present in insufficient amounts in diets given to patients with intestinal dysfunction. In a rat model of total parenteral nutrition and small bowel transplantation, this study investigated the following: (1) whether glutamine improves graft structure and function, (2) the optimal route of glutamine delivery (intravenous vs direct infusion into the graft), and (3) the effect of glutamine on ultrastructure of the graft enterocyte. Lewis rats underwent small bowel transplantation as a Thiry-Vella graft and received total parenteral nutrition for 14 days while assigned to one of four infusion groups: 2% intravenous glutamine; 2% intravenous isonitrogenous mixture, nonessential amino acids (control); 2% glutamine into the graft; or 2% nonessential amino acids into the graft (control). Graft mucosal villous height, villous surface area, crypt depth, weight, protein, deoxyribonucleic acid content, glucose absorption, and enterocyte ultrastructure were then evaluated. Infusion of glutamine directly into the graft significantly increased mucosal villous height (p = .045), surface area (p = .029), and glucose absorption (p = .004) when compared with controls. Intravenous glutamine infusion significantly increased mucosal villous height (p = .002), surface area (p = .001), weight (p = .005), and glucose absorption (p = .04) when compared with controls. Most enterotrophic and functional benefits of glutamine were not significantly different between intravenous infusions and direct administration into the graft.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0148-6071 UR - https://www.unboundmedicine.com/medline/citation/8437324/Glutamine_enhancement_of_structure_and_function_in_transplanted_small_intestine_in_the_rat_ L2 - https://doi.org/10.1177/014860719301700147 DB - PRIME DP - Unbound Medicine ER -