Tags

Type your tag names separated by a space and hit enter

Evidence for an essential non-Watson-Crick interaction between the first and last nucleotides of a nuclear pre-mRNA intron.
Nature. 1993 Feb 18; 361(6413):660-2.Nat

Abstract

Nuclear pre-messenger RNA splicing requires the action of five small nuclear (sn) RNAs, U1, U2, U4, U5 and U6, and more than 50 proteins. The mechanistic similarity of nuclear pre-mRNA splicing and group II self-splicing suggests that many of the central processes of nuclear pre-mRNA splicing are based on RNA-RNA interaction. To understand the mechanism of pre-mRNA splicing, the interactions, and their temporal relationships, that occur between the snRNAs and the pre-mRNA during splicing must be identified. Several snRNA-snRNA and snRNA-intron interactions have been demonstrated but the putative RNA-based interactions that recognize the AG dinucleotide at the 3' splice site during 3' cleavage and exon ligation are unknown. We report here the reciprocal suppression between 5' and 3' splice site mutations in the yeast actin intron, and propose that the 3' splice site is positioned for 3' cleavage and exon ligation, at least in part, through a non-Watson-Crick interaction between the guanosines at the 5' and 3' splice sites.

Authors+Show Affiliations

Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

8437627

Citation

Parker, R, and P G. Siliciano. "Evidence for an Essential non-Watson-Crick Interaction Between the First and Last Nucleotides of a Nuclear pre-mRNA Intron." Nature, vol. 361, no. 6413, 1993, pp. 660-2.
Parker R, Siliciano PG. Evidence for an essential non-Watson-Crick interaction between the first and last nucleotides of a nuclear pre-mRNA intron. Nature. 1993;361(6413):660-2.
Parker, R., & Siliciano, P. G. (1993). Evidence for an essential non-Watson-Crick interaction between the first and last nucleotides of a nuclear pre-mRNA intron. Nature, 361(6413), 660-2.
Parker R, Siliciano PG. Evidence for an Essential non-Watson-Crick Interaction Between the First and Last Nucleotides of a Nuclear pre-mRNA Intron. Nature. 1993 Feb 18;361(6413):660-2. PubMed PMID: 8437627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for an essential non-Watson-Crick interaction between the first and last nucleotides of a nuclear pre-mRNA intron. AU - Parker,R, AU - Siliciano,P G, PY - 1993/2/18/pubmed PY - 1993/2/18/medline PY - 1993/2/18/entrez SP - 660 EP - 2 JF - Nature JO - Nature VL - 361 IS - 6413 N2 - Nuclear pre-messenger RNA splicing requires the action of five small nuclear (sn) RNAs, U1, U2, U4, U5 and U6, and more than 50 proteins. The mechanistic similarity of nuclear pre-mRNA splicing and group II self-splicing suggests that many of the central processes of nuclear pre-mRNA splicing are based on RNA-RNA interaction. To understand the mechanism of pre-mRNA splicing, the interactions, and their temporal relationships, that occur between the snRNAs and the pre-mRNA during splicing must be identified. Several snRNA-snRNA and snRNA-intron interactions have been demonstrated but the putative RNA-based interactions that recognize the AG dinucleotide at the 3' splice site during 3' cleavage and exon ligation are unknown. We report here the reciprocal suppression between 5' and 3' splice site mutations in the yeast actin intron, and propose that the 3' splice site is positioned for 3' cleavage and exon ligation, at least in part, through a non-Watson-Crick interaction between the guanosines at the 5' and 3' splice sites. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/8437627/Evidence_for_an_essential_non_Watson_Crick_interaction_between_the_first_and_last_nucleotides_of_a_nuclear_pre_mRNA_intron_ L2 - https://doi.org/10.1038/361660a0 DB - PRIME DP - Unbound Medicine ER -