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Characterization of the replicon from plasmid pAC1 from Acetobacter pasteurianus.
Biochem Biophys Res Commun. 1993 Feb 26; 191(1):26-31.BB

Abstract

A panel of recombinant plasmids pACK5 and pACT7 was prepared by introducing kanamycin and tetracycline resistance into the partially split plasmid pAC1 which contained replicon isolated from Acetobacter pasteurianus. The replicon in plasmid pAC1 is compatible with the ColE1 replicon. Compared to pBR322, the plasmid had more than 30 copies per chromosome in Escherichia coli cells. Plasmids were transformed into E. coli DH1, Acetobacter pasteurianus 3614, Acetobacter aceti 3620, Shigella, Citrobacter, and Brevibacterium flavum cells, and the stability of plasmid DNA was tested after cultivation in nonselective conditions.

Authors+Show Affiliations

Department of Molecular Biology, Faculty of Natural Science, Comenius University, Bratislava, Czech and Slovak Federal Republic.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8447828

Citation

Grones, J, et al. "Characterization of the Replicon From Plasmid pAC1 From Acetobacter Pasteurianus." Biochemical and Biophysical Research Communications, vol. 191, no. 1, 1993, pp. 26-31.
Grones J, Králová A, Turna J. Characterization of the replicon from plasmid pAC1 from Acetobacter pasteurianus. Biochem Biophys Res Commun. 1993;191(1):26-31.
Grones, J., Králová, A., & Turna, J. (1993). Characterization of the replicon from plasmid pAC1 from Acetobacter pasteurianus. Biochemical and Biophysical Research Communications, 191(1), 26-31.
Grones J, Králová A, Turna J. Characterization of the Replicon From Plasmid pAC1 From Acetobacter Pasteurianus. Biochem Biophys Res Commun. 1993 Feb 26;191(1):26-31. PubMed PMID: 8447828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the replicon from plasmid pAC1 from Acetobacter pasteurianus. AU - Grones,J, AU - Králová,A, AU - Turna,J, PY - 1993/2/26/pubmed PY - 1993/2/26/medline PY - 1993/2/26/entrez SP - 26 EP - 31 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 191 IS - 1 N2 - A panel of recombinant plasmids pACK5 and pACT7 was prepared by introducing kanamycin and tetracycline resistance into the partially split plasmid pAC1 which contained replicon isolated from Acetobacter pasteurianus. The replicon in plasmid pAC1 is compatible with the ColE1 replicon. Compared to pBR322, the plasmid had more than 30 copies per chromosome in Escherichia coli cells. Plasmids were transformed into E. coli DH1, Acetobacter pasteurianus 3614, Acetobacter aceti 3620, Shigella, Citrobacter, and Brevibacterium flavum cells, and the stability of plasmid DNA was tested after cultivation in nonselective conditions. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/8447828/Characterization_of_the_replicon_from_plasmid_pAC1_from_Acetobacter_pasteurianus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(83)71179-4 DB - PRIME DP - Unbound Medicine ER -