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[Analysis of cytoplasmic antigens in acute leukemia by flow cytometry].
Rinsho Ketsueki. 1993 Jan; 34(1):13-20.RK

Abstract

The expression of cytoplasmic antigens in 77 cases of acute leukemia were analyzed by flow cytometry using the following monoclonal antibodies: CD3, CD22, anti-myeloperoxidase (MPO-7) and anti-mu-heavy chain. CD22 antigen was detected in the cytoplasm of all non-T-ALL patients excluding one not-tested patient. In two patients with unclassified ALL, surface CD22 antigen was not expressed but cytoplasmic CD22 antigen was strongly expressed. Three out of 9 patients with common ALL were cytoplasmic mu-heavy chain-positive, so these patients were diagnosed as Pre-B ALL. In four out of 8 patients with T-ALL, CD3 antigen was not expressed on the cell surface membrane. However all of T-ALL patients excluding one non-tested patient were cytoplasmic CD3-positive. The cytoplasmic expression of myeloperoxidase antigen was detected in twenty out of 21 patients with acute non-lymphoblastic leukemia (ANLL). One megakaryocytic leukemia patient was MPO-negative. In two ANLL patients, the percentage of MPO for conventional cytochemical staining was undetectable or low, but MPO antigens were positive (77% and 70%) for flow cytometric analysis. All of 46 non-T ALL patients were cytoplasmic MPO-negative, however 4 out of 10 T-ALL patients were cytoplasmic MPO-positive. The study proved that the analysis of cytoplasmic CD3, CD22, mu-chain and MPO antigens were very useful to define the cell lineage of leukemia and to classify ALL and ANLL. It is necessary to study further whether the expression of MPO in the cytoplasm of T-ALL was non-specific reaction or whether MPO precursors are expressed in the cytoplasm of T-ALL.

Authors+Show Affiliations

Department of Pediatrics, Aichi Medical University.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

jpn

PubMed ID

8450602

Citation

Kawai, S, et al. "[Analysis of Cytoplasmic Antigens in Acute Leukemia By Flow Cytometry]." [Rinsho Ketsueki] the Japanese Journal of Clinical Hematology, vol. 34, no. 1, 1993, pp. 13-20.
Kawai S, Zha Z, Yamamoto Y, et al. [Analysis of cytoplasmic antigens in acute leukemia by flow cytometry]. Rinsho Ketsueki. 1993;34(1):13-20.
Kawai, S., Zha, Z., Yamamoto, Y., Nakadate, H., Kawakami, K., Kikuta, A., Utsumi, J., Nishikawa, K., Mimaya, J., & Miyake, M. (1993). [Analysis of cytoplasmic antigens in acute leukemia by flow cytometry]. [Rinsho Ketsueki] the Japanese Journal of Clinical Hematology, 34(1), 13-20.
Kawai S, et al. [Analysis of Cytoplasmic Antigens in Acute Leukemia By Flow Cytometry]. Rinsho Ketsueki. 1993;34(1):13-20. PubMed PMID: 8450602.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Analysis of cytoplasmic antigens in acute leukemia by flow cytometry]. A1 - Kawai,S, AU - Zha,Z, AU - Yamamoto,Y, AU - Nakadate,H, AU - Kawakami,K, AU - Kikuta,A, AU - Utsumi,J, AU - Nishikawa,K, AU - Mimaya,J, AU - Miyake,M, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 13 EP - 20 JF - [Rinsho ketsueki] The Japanese journal of clinical hematology JO - Rinsho Ketsueki VL - 34 IS - 1 N2 - The expression of cytoplasmic antigens in 77 cases of acute leukemia were analyzed by flow cytometry using the following monoclonal antibodies: CD3, CD22, anti-myeloperoxidase (MPO-7) and anti-mu-heavy chain. CD22 antigen was detected in the cytoplasm of all non-T-ALL patients excluding one not-tested patient. In two patients with unclassified ALL, surface CD22 antigen was not expressed but cytoplasmic CD22 antigen was strongly expressed. Three out of 9 patients with common ALL were cytoplasmic mu-heavy chain-positive, so these patients were diagnosed as Pre-B ALL. In four out of 8 patients with T-ALL, CD3 antigen was not expressed on the cell surface membrane. However all of T-ALL patients excluding one non-tested patient were cytoplasmic CD3-positive. The cytoplasmic expression of myeloperoxidase antigen was detected in twenty out of 21 patients with acute non-lymphoblastic leukemia (ANLL). One megakaryocytic leukemia patient was MPO-negative. In two ANLL patients, the percentage of MPO for conventional cytochemical staining was undetectable or low, but MPO antigens were positive (77% and 70%) for flow cytometric analysis. All of 46 non-T ALL patients were cytoplasmic MPO-negative, however 4 out of 10 T-ALL patients were cytoplasmic MPO-positive. The study proved that the analysis of cytoplasmic CD3, CD22, mu-chain and MPO antigens were very useful to define the cell lineage of leukemia and to classify ALL and ANLL. It is necessary to study further whether the expression of MPO in the cytoplasm of T-ALL was non-specific reaction or whether MPO precursors are expressed in the cytoplasm of T-ALL. SN - 0485-1439 UR - https://www.unboundmedicine.com/medline/citation/8450602/[Analysis_of_cytoplasmic_antigens_in_acute_leukemia_by_flow_cytometry]_ L2 - https://medlineplus.gov/acutemyeloidleukemia.html DB - PRIME DP - Unbound Medicine ER -