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Neurofilament mRNA is reduced in Parkinson's disease substantia nigra pars compacta neurons.
J Comp Neurol. 1993 Mar 15; 329(3):328-36.JC

Abstract

Lewy bodies are filamentous neuronal inclusions characteristic of Parkinson's disease, and neurofilament triplet proteins are the major components of the filaments in Lewy bodies. Since the neurofilament proteins found in Lewy bodies are abnormally phosphorylated and partially degraded, the formation of Lewy bodies may be due to the defective metabolism of these proteins, and this could lead to impairments in the structure and function of neurofilament rich neuronal processes (i.e., large caliber axons). To gain further insights into the metabolism of neurofilaments in Parkinson's disease, we evaluated neurofilament mRNA levels by semi-quantitative in situ hybridization histochemistry in postmortem tissues from Parkinson's disease and control subjects. Substantia nigra pars compacta neurons were examined with digoxigenin-UTP labeled cRNA probes to the heavy and light neurofilament mRNAs. The relative abundance of these mRNAs was measured by videodensitometric image analysis of chromogenic reaction product. Using this approach, we demonstrated that the levels of both heavy and light neurofilament mRNAs were reduced in Parkinson's disease substantia nigra pars compacta neurons. Additionally, the levels of heavy neurofilament mRNA were lowest in Lewy body containing neurons in the Parkinson's disease cases. These results suggest that the formation of neurofilament-rich Lewy bodies in substantia nigra pars compacta neurons is associated with reduced levels of the heavy and light neurofilament mRNAs in Parkinson's disease. Thus, it is possible that the accumulation of abnormal neurofilament proteins in Lewy bodies and diminished neurofilament mRNAs contribute to the degeneration of substantia nigra pars compacta neurons in Parkinson's disease.

Authors+Show Affiliations

Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 30912-2000.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8459049

Citation

Hill, W D., et al. "Neurofilament mRNA Is Reduced in Parkinson's Disease Substantia Nigra Pars Compacta Neurons." The Journal of Comparative Neurology, vol. 329, no. 3, 1993, pp. 328-36.
Hill WD, Arai M, Cohen JA, et al. Neurofilament mRNA is reduced in Parkinson's disease substantia nigra pars compacta neurons. J Comp Neurol. 1993;329(3):328-36.
Hill, W. D., Arai, M., Cohen, J. A., & Trojanowski, J. Q. (1993). Neurofilament mRNA is reduced in Parkinson's disease substantia nigra pars compacta neurons. The Journal of Comparative Neurology, 329(3), 328-36.
Hill WD, et al. Neurofilament mRNA Is Reduced in Parkinson's Disease Substantia Nigra Pars Compacta Neurons. J Comp Neurol. 1993 Mar 15;329(3):328-36. PubMed PMID: 8459049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neurofilament mRNA is reduced in Parkinson's disease substantia nigra pars compacta neurons. AU - Hill,W D, AU - Arai,M, AU - Cohen,J A, AU - Trojanowski,J Q, PY - 1993/3/15/pubmed PY - 1993/3/15/medline PY - 1993/3/15/entrez SP - 328 EP - 36 JF - The Journal of comparative neurology JO - J Comp Neurol VL - 329 IS - 3 N2 - Lewy bodies are filamentous neuronal inclusions characteristic of Parkinson's disease, and neurofilament triplet proteins are the major components of the filaments in Lewy bodies. Since the neurofilament proteins found in Lewy bodies are abnormally phosphorylated and partially degraded, the formation of Lewy bodies may be due to the defective metabolism of these proteins, and this could lead to impairments in the structure and function of neurofilament rich neuronal processes (i.e., large caliber axons). To gain further insights into the metabolism of neurofilaments in Parkinson's disease, we evaluated neurofilament mRNA levels by semi-quantitative in situ hybridization histochemistry in postmortem tissues from Parkinson's disease and control subjects. Substantia nigra pars compacta neurons were examined with digoxigenin-UTP labeled cRNA probes to the heavy and light neurofilament mRNAs. The relative abundance of these mRNAs was measured by videodensitometric image analysis of chromogenic reaction product. Using this approach, we demonstrated that the levels of both heavy and light neurofilament mRNAs were reduced in Parkinson's disease substantia nigra pars compacta neurons. Additionally, the levels of heavy neurofilament mRNA were lowest in Lewy body containing neurons in the Parkinson's disease cases. These results suggest that the formation of neurofilament-rich Lewy bodies in substantia nigra pars compacta neurons is associated with reduced levels of the heavy and light neurofilament mRNAs in Parkinson's disease. Thus, it is possible that the accumulation of abnormal neurofilament proteins in Lewy bodies and diminished neurofilament mRNAs contribute to the degeneration of substantia nigra pars compacta neurons in Parkinson's disease. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/8459049/Neurofilament_mRNA_is_reduced_in_Parkinson's_disease_substantia_nigra_pars_compacta_neurons_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1993&volume=329&issue=3&spage=328 DB - PRIME DP - Unbound Medicine ER -