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Molecular cloning and expression of the gene for serine hydroxymethyltransferase from an obligate methylotroph Hyphomicrobium methylovorum GM2.
Eur J Biochem. 1993 Mar 15; 212(3):745-50.EJ

Abstract

The gene encoding serine hydroxymethyltransferase (SHMT), one of the key enzymes of the one-carbon-compound assimilation of a methylotroph, Hyphomicrobium methylovorum GM2, and its flanking regions were isolated using a DNA fragment encoding Escherichia coli SHMT as a probe. Nucleotide sequencing of the recombinant plasmids revealed the SHMT gene codes for the 434-amino-acid protein with a calculated molecular mass of 46,068 Da. The amino-acid sequence of the enzyme showed identity to the sequences of the enzymes from E. coli (55%) and rabbit liver (44%). The recombinant plasmid, which was constructed by ligation of the cloned gene and an expression vector pKK223-3, was introduced to an SHMT-deficient E. coli mutant ME5427 (glyA-). The transformed E. coli cells expressed SHMT, which was immunologically and enzymologically indistinguishable from the enzyme isolated from H. methylovorum GM2.

Authors+Show Affiliations

Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8462546

Citation

Miyata, A, et al. "Molecular Cloning and Expression of the Gene for Serine Hydroxymethyltransferase From an Obligate Methylotroph Hyphomicrobium Methylovorum GM2." European Journal of Biochemistry, vol. 212, no. 3, 1993, pp. 745-50.
Miyata A, Yoshida T, Yamaguchi K, et al. Molecular cloning and expression of the gene for serine hydroxymethyltransferase from an obligate methylotroph Hyphomicrobium methylovorum GM2. Eur J Biochem. 1993;212(3):745-50.
Miyata, A., Yoshida, T., Yamaguchi, K., Yokoyama, C., Tanabe, T., Toh, H., Mitsunaga, T., & Izumi, Y. (1993). Molecular cloning and expression of the gene for serine hydroxymethyltransferase from an obligate methylotroph Hyphomicrobium methylovorum GM2. European Journal of Biochemistry, 212(3), 745-50.
Miyata A, et al. Molecular Cloning and Expression of the Gene for Serine Hydroxymethyltransferase From an Obligate Methylotroph Hyphomicrobium Methylovorum GM2. Eur J Biochem. 1993 Mar 15;212(3):745-50. PubMed PMID: 8462546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and expression of the gene for serine hydroxymethyltransferase from an obligate methylotroph Hyphomicrobium methylovorum GM2. AU - Miyata,A, AU - Yoshida,T, AU - Yamaguchi,K, AU - Yokoyama,C, AU - Tanabe,T, AU - Toh,H, AU - Mitsunaga,T, AU - Izumi,Y, PY - 1993/3/15/pubmed PY - 1993/3/15/medline PY - 1993/3/15/entrez SP - 745 EP - 50 JF - European journal of biochemistry JO - Eur. J. Biochem. VL - 212 IS - 3 N2 - The gene encoding serine hydroxymethyltransferase (SHMT), one of the key enzymes of the one-carbon-compound assimilation of a methylotroph, Hyphomicrobium methylovorum GM2, and its flanking regions were isolated using a DNA fragment encoding Escherichia coli SHMT as a probe. Nucleotide sequencing of the recombinant plasmids revealed the SHMT gene codes for the 434-amino-acid protein with a calculated molecular mass of 46,068 Da. The amino-acid sequence of the enzyme showed identity to the sequences of the enzymes from E. coli (55%) and rabbit liver (44%). The recombinant plasmid, which was constructed by ligation of the cloned gene and an expression vector pKK223-3, was introduced to an SHMT-deficient E. coli mutant ME5427 (glyA-). The transformed E. coli cells expressed SHMT, which was immunologically and enzymologically indistinguishable from the enzyme isolated from H. methylovorum GM2. SN - 0014-2956 UR - https://www.unboundmedicine.com/medline/citation/8462546/Molecular_cloning_and_expression_of_the_gene_for_serine_hydroxymethyltransferase_from_an_obligate_methylotroph_Hyphomicrobium_methylovorum_GM2_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2956&date=1993&volume=212&issue=3&spage=745 DB - PRIME DP - Unbound Medicine ER -