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CD22, a B cell-specific immunoglobulin superfamily member, is a sialic acid-binding lectin.
J Biol Chem. 1993 Apr 05; 268(10):7011-8.JB

Abstract

The B lymphocyte cell surface receptor CD22 is an adhesion molecule that can mediate binding to several leukocyte subsets. The first CD22 ligand to be identified was the receptor-linked phosphotyrosine phosphatase CD45, but several lines of evidence suggest that CD22 may interact with multiple counter receptors on adjacent lymphocytes. In the present work, we show that in addition to CD45, a soluble CD22-immunoglobulin fusion protein (CD22Rg) recognizes several other distinct lymphocyte sialoglycoproteins. CD22-mediated adhesion is dependent upon the presence of sialic acids on ligands. CD22Rg is observed to bind specifically to a 115-kDa sialoglycoprotein in COS cells transfected with an alpha-2,6-sialyltransferase cDNA, but not in COS cells transfected with unrelated cDNA clones, indicating that at least some CD22-mediated interactions require presentation of sialic acid in an alpha-2,6 linkage by CD22 ligands. In all cases, truncation of the side chain of sialic acids by mild periodate oxidation abolishes recognition by CD22Rg. Direct binding of CD22Rg to lymphoid cells also requires sialic acids and their side chains. Taken together, these observations indicate that CD22 is a sialic acid-binding lectin and may define a novel functional subset of immunoglobulin superfamily adhesion molecules.

Authors+Show Affiliations

Department of Pathology, Massachusetts General Hospital, Charlestown 02129.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8463234

Citation

Sgroi, D, et al. "CD22, a B Cell-specific Immunoglobulin Superfamily Member, Is a Sialic Acid-binding Lectin." The Journal of Biological Chemistry, vol. 268, no. 10, 1993, pp. 7011-8.
Sgroi D, Varki A, Braesch-Andersen S, et al. CD22, a B cell-specific immunoglobulin superfamily member, is a sialic acid-binding lectin. J Biol Chem. 1993;268(10):7011-8.
Sgroi, D., Varki, A., Braesch-Andersen, S., & Stamenkovic, I. (1993). CD22, a B cell-specific immunoglobulin superfamily member, is a sialic acid-binding lectin. The Journal of Biological Chemistry, 268(10), 7011-8.
Sgroi D, et al. CD22, a B Cell-specific Immunoglobulin Superfamily Member, Is a Sialic Acid-binding Lectin. J Biol Chem. 1993 Apr 5;268(10):7011-8. PubMed PMID: 8463234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD22, a B cell-specific immunoglobulin superfamily member, is a sialic acid-binding lectin. AU - Sgroi,D, AU - Varki,A, AU - Braesch-Andersen,S, AU - Stamenkovic,I, PY - 1993/4/5/pubmed PY - 1993/4/5/medline PY - 1993/4/5/entrez SP - 7011 EP - 8 JF - The Journal of biological chemistry JO - J Biol Chem VL - 268 IS - 10 N2 - The B lymphocyte cell surface receptor CD22 is an adhesion molecule that can mediate binding to several leukocyte subsets. The first CD22 ligand to be identified was the receptor-linked phosphotyrosine phosphatase CD45, but several lines of evidence suggest that CD22 may interact with multiple counter receptors on adjacent lymphocytes. In the present work, we show that in addition to CD45, a soluble CD22-immunoglobulin fusion protein (CD22Rg) recognizes several other distinct lymphocyte sialoglycoproteins. CD22-mediated adhesion is dependent upon the presence of sialic acids on ligands. CD22Rg is observed to bind specifically to a 115-kDa sialoglycoprotein in COS cells transfected with an alpha-2,6-sialyltransferase cDNA, but not in COS cells transfected with unrelated cDNA clones, indicating that at least some CD22-mediated interactions require presentation of sialic acid in an alpha-2,6 linkage by CD22 ligands. In all cases, truncation of the side chain of sialic acids by mild periodate oxidation abolishes recognition by CD22Rg. Direct binding of CD22Rg to lymphoid cells also requires sialic acids and their side chains. Taken together, these observations indicate that CD22 is a sialic acid-binding lectin and may define a novel functional subset of immunoglobulin superfamily adhesion molecules. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/8463234/CD22_a_B_cell_specific_immunoglobulin_superfamily_member_is_a_sialic_acid_binding_lectin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(18)53139-2 DB - PRIME DP - Unbound Medicine ER -