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Natural ligands of the B cell adhesion molecule CD22 beta carry N-linked oligosaccharides with alpha-2,6-linked sialic acids that are required for recognition.
J Biol Chem. 1993 Apr 05; 268(10):7019-27.JB

Abstract

CD22 beta is a glycoprotein found on the surface of B cells during restricted stages of development. It is believed to play a role in cell-cell interactions and B cell activation. The accompanying paper (Sgroi, D., Varki, A., Braesch-Andersen, S., and Stamenkovic, I. (1993) J. Biol. Chem. 268, 7011-7018) shows that CD22 beta recognizes multiple glycoproteins on the surfaces of T and B cells and that sialylation of these ligands is essential for binding. To identify the structure(s) of the sialylated oligosaccharide(s) recognized by CD22 beta, [3H]glucosamine-labeled glycoproteins were purified from Daudi cells by adsorption onto a CD22 beta recombinant immunoglobulin (CD22 beta Rg) chimera attached to protein A-Sepharose (PAS), and the N-linked oligosaccharides were released by peptide N-glycosidase F. These released oligosaccharides failed to bind to CD22 beta Rg-PAS under the conditions used initially to adsorb the glycoproteins, but their elution from a column of CD22 beta Rg-PAS was significantly retarded. Populations of oligosaccharides with different affinities could be identified by their order of elution. Specific sialidases were used to determine the content of alpha-2,3- and alpha-2,6-linked sialic acid in these different populations and their contribution to binding. Multiantennary oligosaccharides with one alpha-2,6-linked residue bound marginally, and those with two or more bound more tightly. alpha-2,3-Linked sialic acid residues were without effect. Binding did not require divalent cations and was abrogated by mild periodate oxidation of the outer side chain of sialic acid. No marked differences in size or fucose content were found between the populations of high and low affinity oligosaccharides. However, the low affinity population could be partially converted into higher affinity by treatment with beta-galactoside alpha-2,6 sialyltransferase and CMP-sialic acid. Thus, CD22 beta is a mammalian lectin that can recognize specific N-linked oligosaccharide structures containing alpha-2,6-linked sialic acids.

Authors+Show Affiliations

Glycobiology Program, University of California, La Jolla 92093-0063.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8463235

Citation

Powell, L D., et al. "Natural Ligands of the B Cell Adhesion Molecule CD22 Beta Carry N-linked Oligosaccharides With Alpha-2,6-linked Sialic Acids That Are Required for Recognition." The Journal of Biological Chemistry, vol. 268, no. 10, 1993, pp. 7019-27.
Powell LD, Sgroi D, Sjoberg ER, et al. Natural ligands of the B cell adhesion molecule CD22 beta carry N-linked oligosaccharides with alpha-2,6-linked sialic acids that are required for recognition. J Biol Chem. 1993;268(10):7019-27.
Powell, L. D., Sgroi, D., Sjoberg, E. R., Stamenkovic, I., & Varki, A. (1993). Natural ligands of the B cell adhesion molecule CD22 beta carry N-linked oligosaccharides with alpha-2,6-linked sialic acids that are required for recognition. The Journal of Biological Chemistry, 268(10), 7019-27.
Powell LD, et al. Natural Ligands of the B Cell Adhesion Molecule CD22 Beta Carry N-linked Oligosaccharides With Alpha-2,6-linked Sialic Acids That Are Required for Recognition. J Biol Chem. 1993 Apr 5;268(10):7019-27. PubMed PMID: 8463235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural ligands of the B cell adhesion molecule CD22 beta carry N-linked oligosaccharides with alpha-2,6-linked sialic acids that are required for recognition. AU - Powell,L D, AU - Sgroi,D, AU - Sjoberg,E R, AU - Stamenkovic,I, AU - Varki,A, PY - 1993/4/5/pubmed PY - 1993/4/5/medline PY - 1993/4/5/entrez SP - 7019 EP - 27 JF - The Journal of biological chemistry JO - J Biol Chem VL - 268 IS - 10 N2 - CD22 beta is a glycoprotein found on the surface of B cells during restricted stages of development. It is believed to play a role in cell-cell interactions and B cell activation. The accompanying paper (Sgroi, D., Varki, A., Braesch-Andersen, S., and Stamenkovic, I. (1993) J. Biol. Chem. 268, 7011-7018) shows that CD22 beta recognizes multiple glycoproteins on the surfaces of T and B cells and that sialylation of these ligands is essential for binding. To identify the structure(s) of the sialylated oligosaccharide(s) recognized by CD22 beta, [3H]glucosamine-labeled glycoproteins were purified from Daudi cells by adsorption onto a CD22 beta recombinant immunoglobulin (CD22 beta Rg) chimera attached to protein A-Sepharose (PAS), and the N-linked oligosaccharides were released by peptide N-glycosidase F. These released oligosaccharides failed to bind to CD22 beta Rg-PAS under the conditions used initially to adsorb the glycoproteins, but their elution from a column of CD22 beta Rg-PAS was significantly retarded. Populations of oligosaccharides with different affinities could be identified by their order of elution. Specific sialidases were used to determine the content of alpha-2,3- and alpha-2,6-linked sialic acid in these different populations and their contribution to binding. Multiantennary oligosaccharides with one alpha-2,6-linked residue bound marginally, and those with two or more bound more tightly. alpha-2,3-Linked sialic acid residues were without effect. Binding did not require divalent cations and was abrogated by mild periodate oxidation of the outer side chain of sialic acid. No marked differences in size or fucose content were found between the populations of high and low affinity oligosaccharides. However, the low affinity population could be partially converted into higher affinity by treatment with beta-galactoside alpha-2,6 sialyltransferase and CMP-sialic acid. Thus, CD22 beta is a mammalian lectin that can recognize specific N-linked oligosaccharide structures containing alpha-2,6-linked sialic acids. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/8463235/Natural_ligands_of_the_B_cell_adhesion_molecule_CD22_beta_carry_N_linked_oligosaccharides_with_alpha_26_linked_sialic_acids_that_are_required_for_recognition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(18)53140-9 DB - PRIME DP - Unbound Medicine ER -