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IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction.
Am Rev Respir Dis. 1993 Apr; 147(4):901-7.AR

Abstract

The mechanism of airway eosinophilia during antigen-induced inflammation was investigated by measurement of eosinophil-active cytokines utilizing an eosinophil survival assay. In the first study, 4 patients with allergic rhinitis underwent segmental bronchoprovocation (SBP) with low, medium, and high doses of ragweed extract instilled into different bronchial subsegments; bronchoalveolar lavage (BAL) fluids were collected from each segment 12 min and 48 h after challenge. Eosinophil granule proteins and eosinophil survival activity were significantly elevated in the 48-h (late-phase) BAL fluids from these segments. Correlations were observed between the concentrations of eosinophil granule proteins and eosinophil survival activity (rs = 0.717 to 0.880, p < 0.001) in BAL fluids. Eosinophil survival activity was completely neutralized by anti-IL-5 monoclonal antibody in five of the seven 48-h samples tested representing three of the 4 patients. In the two remaining samples, eosinophil survival activity was only partially neutralized by either anti-IL-5 antibody or anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) but was completely neutralized by anti-IL-5 and anti-GM-CSF in combination. Subsequently, in the second study, 10 patients with allergic rhinitis were challenged by SBP with ragweed extract. Eosinophil survival activity was significantly elevated in the 48-h BAL fluids; this activity was partially neutralized by anti-IL-5 antibody about (48%) and completely neutralized by the combination of anti-IL-5 and anti-GM-CSF antibodies. These findings suggest that the eosinophil survival activity in the late inflammatory lesions following SBP with allergen is mainly associated with IL-5, with small contributions from GM-CSF.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Immunology, Mayo Clinic, Rochester, MN 55905.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8466126

Citation

Ohnishi, T, et al. "IL-5 Is the Predominant Eosinophil-active Cytokine in the Antigen-induced Pulmonary Late-phase Reaction." The American Review of Respiratory Disease, vol. 147, no. 4, 1993, pp. 901-7.
Ohnishi T, Kita H, Weiler D, et al. IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction. Am Rev Respir Dis. 1993;147(4):901-7.
Ohnishi, T., Kita, H., Weiler, D., Sur, S., Sedgwick, J. B., Calhoun, W. J., Busse, W. W., Abrams, J. S., & Gleich, G. J. (1993). IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction. The American Review of Respiratory Disease, 147(4), 901-7.
Ohnishi T, et al. IL-5 Is the Predominant Eosinophil-active Cytokine in the Antigen-induced Pulmonary Late-phase Reaction. Am Rev Respir Dis. 1993;147(4):901-7. PubMed PMID: 8466126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction. AU - Ohnishi,T, AU - Kita,H, AU - Weiler,D, AU - Sur,S, AU - Sedgwick,J B, AU - Calhoun,W J, AU - Busse,W W, AU - Abrams,J S, AU - Gleich,G J, PY - 1993/4/1/pubmed PY - 1993/4/1/medline PY - 1993/4/1/entrez SP - 901 EP - 7 JF - The American review of respiratory disease JO - Am Rev Respir Dis VL - 147 IS - 4 N2 - The mechanism of airway eosinophilia during antigen-induced inflammation was investigated by measurement of eosinophil-active cytokines utilizing an eosinophil survival assay. In the first study, 4 patients with allergic rhinitis underwent segmental bronchoprovocation (SBP) with low, medium, and high doses of ragweed extract instilled into different bronchial subsegments; bronchoalveolar lavage (BAL) fluids were collected from each segment 12 min and 48 h after challenge. Eosinophil granule proteins and eosinophil survival activity were significantly elevated in the 48-h (late-phase) BAL fluids from these segments. Correlations were observed between the concentrations of eosinophil granule proteins and eosinophil survival activity (rs = 0.717 to 0.880, p < 0.001) in BAL fluids. Eosinophil survival activity was completely neutralized by anti-IL-5 monoclonal antibody in five of the seven 48-h samples tested representing three of the 4 patients. In the two remaining samples, eosinophil survival activity was only partially neutralized by either anti-IL-5 antibody or anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) but was completely neutralized by anti-IL-5 and anti-GM-CSF in combination. Subsequently, in the second study, 10 patients with allergic rhinitis were challenged by SBP with ragweed extract. Eosinophil survival activity was significantly elevated in the 48-h BAL fluids; this activity was partially neutralized by anti-IL-5 antibody about (48%) and completely neutralized by the combination of anti-IL-5 and anti-GM-CSF antibodies. These findings suggest that the eosinophil survival activity in the late inflammatory lesions following SBP with allergen is mainly associated with IL-5, with small contributions from GM-CSF.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0003-0805 UR - https://www.unboundmedicine.com/medline/citation/8466126/IL_5_is_the_predominant_eosinophil_active_cytokine_in_the_antigen_induced_pulmonary_late_phase_reaction_ L2 - https://www.atsjournals.org/doi/10.1164/ajrccm/147.4.901?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -