Tags

Type your tag names separated by a space and hit enter

A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coli otsA mutant.
FEMS Microbiol Lett 1993; 107(1):25-30FM

Abstract

A Saccharomyces cerevisiae gene for trehalose-6-phosphate synthase (TPS1) was sequenced. The gene appeared to code for a protein of 495 amino acid residues, giving the protein a molecular mass of 56 kDa. The TPS1 gene was able to restore both osmotolerance and trehalose accumulation during salt stress in an Escherichia coli strain mutated in the otsA gene encoding trehalose-6-phosphate synthase. Complementation studies with E. coli galU mutants showed that the TPS1-encoded trehalose-6-phosphate synthase is UDP-glucose-dependent. Sequence analysis and data base searches showed that TPS1 is allelic to GGS1, byp1, cif1 and fdp1. A possible gene for trehalose-6-phosphate synthase in Methanobacterium thermoautotrophicum was identified.

Authors+Show Affiliations

Norwegian College of Fishery Science, University of Tromsø.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8467996

Citation

McDougall, J, et al. "A Yeast Gene for Trehalose-6-phosphate Synthase and Its Complementation of an Escherichia Coli otsA Mutant." FEMS Microbiology Letters, vol. 107, no. 1, 1993, pp. 25-30.
McDougall J, Kaasen I, Strøm AR. A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coli otsA mutant. FEMS Microbiol Lett. 1993;107(1):25-30.
McDougall, J., Kaasen, I., & Strøm, A. R. (1993). A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coli otsA mutant. FEMS Microbiology Letters, 107(1), pp. 25-30.
McDougall J, Kaasen I, Strøm AR. A Yeast Gene for Trehalose-6-phosphate Synthase and Its Complementation of an Escherichia Coli otsA Mutant. FEMS Microbiol Lett. 1993 Feb 15;107(1):25-30. PubMed PMID: 8467996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coli otsA mutant. AU - McDougall,J, AU - Kaasen,I, AU - Strøm,A R, PY - 1993/2/15/pubmed PY - 1993/2/15/medline PY - 1993/2/15/entrez SP - 25 EP - 30 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 107 IS - 1 N2 - A Saccharomyces cerevisiae gene for trehalose-6-phosphate synthase (TPS1) was sequenced. The gene appeared to code for a protein of 495 amino acid residues, giving the protein a molecular mass of 56 kDa. The TPS1 gene was able to restore both osmotolerance and trehalose accumulation during salt stress in an Escherichia coli strain mutated in the otsA gene encoding trehalose-6-phosphate synthase. Complementation studies with E. coli galU mutants showed that the TPS1-encoded trehalose-6-phosphate synthase is UDP-glucose-dependent. Sequence analysis and data base searches showed that TPS1 is allelic to GGS1, byp1, cif1 and fdp1. A possible gene for trehalose-6-phosphate synthase in Methanobacterium thermoautotrophicum was identified. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/8467996/A_yeast_gene_for_trehalose_6_phosphate_synthase_and_its_complementation_of_an_Escherichia_coli_otsA_mutant_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1016/0378-1097(93)90348-6 DB - PRIME DP - Unbound Medicine ER -