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Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats.
Exp Eye Res 1993; 56(3):341-7EE

Abstract

Capillary closure and venous dilatation occur early in diabetic retinopathy, and altered blood rheology may play a role. For example previous studies have shown leukocytes are less deformable, are more activated, and occlude retinal capillaries more often in diabetic subjects. The purpose of this study was to determine if rheologic changes developed in diabetic cats, a model in which we have documented retinal capillary basement membrane thickening, microaneurysms, and intraretinal hemorrhage characteristics of early diabetic retinopathy. Viscosity of blood, plasma and purified erythrocyte suspensions was measured by rotational viscometry and plasma fibrinogen content was determined by heat precipitation. Filterability of blood and purified erythrocyte, mononuclear leukocyte, and granulocyte suspensions was determined at constant flow, measuring the increase in pressure over 2 min relative to the pressure generated by buffer alone. Viscosity of whole blood and plasma, but not erythrocytes, was significantly elevated (P < 0.005) in the diabetic cats over normals at all shear rates studied (450, 225 and 90 sec-1). Furthermore, fibrinogen levels were significantly elevated in diabetic cats compared to normals (P < 0.004), and were positively correlated with the viscosity of whole blood and plasma. The filterability of mononuclear leukocytes and whole blood in diabetic cats was decreased 56% and 74% when compared to normals, P < 0.0006 and P < 0.025, respectively. In contrast, the filterability of granulocytes and erythrocytes was not significantly different between the two groups. These results suggest that the rheologic alterations in diabetes are numerous, and involve both cellular and plasma protein changes.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8472789

Citation

Kelly, L W., et al. "Alterations in Viscosity and Filterability of Whole Blood and Blood Cell Subpopulations in Diabetic Cats." Experimental Eye Research, vol. 56, no. 3, 1993, pp. 341-7.
Kelly LW, Barden CA, Tiedeman JS, et al. Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats. Exp Eye Res. 1993;56(3):341-7.
Kelly, L. W., Barden, C. A., Tiedeman, J. S., & Hatchell, D. L. (1993). Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats. Experimental Eye Research, 56(3), pp. 341-7.
Kelly LW, et al. Alterations in Viscosity and Filterability of Whole Blood and Blood Cell Subpopulations in Diabetic Cats. Exp Eye Res. 1993;56(3):341-7. PubMed PMID: 8472789.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alterations in viscosity and filterability of whole blood and blood cell subpopulations in diabetic cats. AU - Kelly,L W, AU - Barden,C A, AU - Tiedeman,J S, AU - Hatchell,D L, PY - 1993/3/1/pubmed PY - 1993/3/1/medline PY - 1993/3/1/entrez SP - 341 EP - 7 JF - Experimental eye research JO - Exp. Eye Res. VL - 56 IS - 3 N2 - Capillary closure and venous dilatation occur early in diabetic retinopathy, and altered blood rheology may play a role. For example previous studies have shown leukocytes are less deformable, are more activated, and occlude retinal capillaries more often in diabetic subjects. The purpose of this study was to determine if rheologic changes developed in diabetic cats, a model in which we have documented retinal capillary basement membrane thickening, microaneurysms, and intraretinal hemorrhage characteristics of early diabetic retinopathy. Viscosity of blood, plasma and purified erythrocyte suspensions was measured by rotational viscometry and plasma fibrinogen content was determined by heat precipitation. Filterability of blood and purified erythrocyte, mononuclear leukocyte, and granulocyte suspensions was determined at constant flow, measuring the increase in pressure over 2 min relative to the pressure generated by buffer alone. Viscosity of whole blood and plasma, but not erythrocytes, was significantly elevated (P < 0.005) in the diabetic cats over normals at all shear rates studied (450, 225 and 90 sec-1). Furthermore, fibrinogen levels were significantly elevated in diabetic cats compared to normals (P < 0.004), and were positively correlated with the viscosity of whole blood and plasma. The filterability of mononuclear leukocytes and whole blood in diabetic cats was decreased 56% and 74% when compared to normals, P < 0.0006 and P < 0.025, respectively. In contrast, the filterability of granulocytes and erythrocytes was not significantly different between the two groups. These results suggest that the rheologic alterations in diabetes are numerous, and involve both cellular and plasma protein changes.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0014-4835 UR - https://www.unboundmedicine.com/medline/citation/8472789/Alterations_in_viscosity_and_filterability_of_whole_blood_and_blood_cell_subpopulations_in_diabetic_cats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(83)71044-4 DB - PRIME DP - Unbound Medicine ER -