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Gastrin-releasing peptide-like immunoreactive substance in bronchoalveolar lavage of idiopathic pulmonary fibrosis and sarcoidosis.
Eur Respir J. 1993 Mar; 6(3):418-24.ER

Abstract

The neuropeptide gastrin releasing peptide (GRP) is present in the lung, and functions as a modulator of tissue growth and repair in fibrotic processes, or as a modulator of cell movement and differentiation in various inflammatory processes, including granulomatous ones. In idiopathic pulmonary fibrosis (IPF), changes in the bronchoalveolar lavage (BAL) content of GRP can be expected. We measured GRP-like immunoreactive substances (GRP-IS) and another neuropeptide, vasoactive intestinal peptide (VIP)-IS in BAL by enzyme immunoassay. Our results showed a decrease in BAL GRP-IS in patients with IPF (26.5 +/- 5.5 pg.mg-1 protein) and sarcoidosis (35.9 +/- 9.2 pg.mg-1), compared to healthy nonsmokers (63.4 +/- 9.0 pg.mg-1). When data were expressed as pg.ml-1 BAL fluid recovered, a decrease was only seen in IPF, not in sarcoidosis. The levels of VIP-IS in BAL were not different between the groups studied. Increased protein levels in BAL had no correlation with the levels of GRP-IS or VIP-IS in BAL. Furthermore, BAL neutrophil percentages had no correlation with the levels of GRP-IS in BAL of patients with IPF. Using reversed phase high performance liquid chromatography (HPLC), several kinds of GRP-IS were detected in BAL. These findings suggest that the decreased level of GRP-IS in BAL may reflect a loss of GRP-producing cells due to chronic lung injury and fibrosis in patients with IPF.

Authors+Show Affiliations

Dept of Clinical Pharmacy, Oita Medical University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8472833

Citation

Takeyama, M, et al. "Gastrin-releasing Peptide-like Immunoreactive Substance in Bronchoalveolar Lavage of Idiopathic Pulmonary Fibrosis and Sarcoidosis." The European Respiratory Journal, vol. 6, no. 3, 1993, pp. 418-24.
Takeyama M, Nagai S, Kondo K, et al. Gastrin-releasing peptide-like immunoreactive substance in bronchoalveolar lavage of idiopathic pulmonary fibrosis and sarcoidosis. Eur Respir J. 1993;6(3):418-24.
Takeyama, M., Nagai, S., Kondo, K., Morikawa, N., Mio, T., Satake, N., Kitaichi, M., & Izumi, T. (1993). Gastrin-releasing peptide-like immunoreactive substance in bronchoalveolar lavage of idiopathic pulmonary fibrosis and sarcoidosis. The European Respiratory Journal, 6(3), 418-24.
Takeyama M, et al. Gastrin-releasing Peptide-like Immunoreactive Substance in Bronchoalveolar Lavage of Idiopathic Pulmonary Fibrosis and Sarcoidosis. Eur Respir J. 1993;6(3):418-24. PubMed PMID: 8472833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastrin-releasing peptide-like immunoreactive substance in bronchoalveolar lavage of idiopathic pulmonary fibrosis and sarcoidosis. AU - Takeyama,M, AU - Nagai,S, AU - Kondo,K, AU - Morikawa,N, AU - Mio,T, AU - Satake,N, AU - Kitaichi,M, AU - Izumi,T, PY - 1993/3/1/pubmed PY - 1993/3/1/medline PY - 1993/3/1/entrez SP - 418 EP - 24 JF - The European respiratory journal JO - Eur Respir J VL - 6 IS - 3 N2 - The neuropeptide gastrin releasing peptide (GRP) is present in the lung, and functions as a modulator of tissue growth and repair in fibrotic processes, or as a modulator of cell movement and differentiation in various inflammatory processes, including granulomatous ones. In idiopathic pulmonary fibrosis (IPF), changes in the bronchoalveolar lavage (BAL) content of GRP can be expected. We measured GRP-like immunoreactive substances (GRP-IS) and another neuropeptide, vasoactive intestinal peptide (VIP)-IS in BAL by enzyme immunoassay. Our results showed a decrease in BAL GRP-IS in patients with IPF (26.5 +/- 5.5 pg.mg-1 protein) and sarcoidosis (35.9 +/- 9.2 pg.mg-1), compared to healthy nonsmokers (63.4 +/- 9.0 pg.mg-1). When data were expressed as pg.ml-1 BAL fluid recovered, a decrease was only seen in IPF, not in sarcoidosis. The levels of VIP-IS in BAL were not different between the groups studied. Increased protein levels in BAL had no correlation with the levels of GRP-IS or VIP-IS in BAL. Furthermore, BAL neutrophil percentages had no correlation with the levels of GRP-IS in BAL of patients with IPF. Using reversed phase high performance liquid chromatography (HPLC), several kinds of GRP-IS were detected in BAL. These findings suggest that the decreased level of GRP-IS in BAL may reflect a loss of GRP-producing cells due to chronic lung injury and fibrosis in patients with IPF. SN - 0903-1936 UR - https://www.unboundmedicine.com/medline/citation/8472833/Gastrin_releasing_peptide_like_immunoreactive_substance_in_bronchoalveolar_lavage_of_idiopathic_pulmonary_fibrosis_and_sarcoidosis_ L2 - http://www.diseaseinfosearch.org/result/6087 DB - PRIME DP - Unbound Medicine ER -