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More basic forms of both human follicle-stimulating hormone and luteinizing hormone in serum at midcycle compared with the follicular or luteal phase.
J Clin Endocrinol Metab. 1993 Apr; 76(4):885-9.JC

Abstract

The purpose of this study was to compare the heterogeneity and median charge of FSH and LH in serum at different phases of the normal human menstrual cycle. Serum specimens were obtained during the follicular phase [9.0 +/- 1.9 (+/- SD) days before the midcycle], at the midcycle LH peak, and during the luteal phase (9.75 +/- 1.6 days after the midcycle) in 16 women with normal menstrual cycles. The 48 serum specimens were subjected to electrophoresis in 0.17% agarose suspension in 0.075 M veronal buffer at pH 8.6, using a column suitable for measuring the median charge of the isoforms of FSH and LH, expressed as median electrophoretic mobility. Four sera were also analyzed by use of a larger column, giving a high resolution. The FSH and LH activities were measured with a time-resolved sandwich fluoroimmunoassay. The number of isoforms of both FSH and LH in each serum specimen analyzed by electrophoresis with high resolution was between 20-30. The median charge of the isoforms of FSH was less negative (P < 0.001) at the midcycle than in the follicular or luteal phase in all 16 women. The same was found for LH in 14 of 15 women. The median charge of FSH or LH in the follicular phase was not significantly different from that in the luteal phase of the cycle. We conclude that at least 20-30 isoforms of both FSH and LH circulate in blood during the menstrual cycle. More basic isoforms of both hormones appear in serum at midcycle than in the follicular or luteal phase. The difference is most likely due to a selective secretion from the pituitary of more basic forms of FSH and LH at midcycle.

Authors+Show Affiliations

Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8473400

Citation

Wide, L, and O Bakos. "More Basic Forms of Both Human Follicle-stimulating Hormone and Luteinizing Hormone in Serum at Midcycle Compared With the Follicular or Luteal Phase." The Journal of Clinical Endocrinology and Metabolism, vol. 76, no. 4, 1993, pp. 885-9.
Wide L, Bakos O. More basic forms of both human follicle-stimulating hormone and luteinizing hormone in serum at midcycle compared with the follicular or luteal phase. J Clin Endocrinol Metab. 1993;76(4):885-9.
Wide, L., & Bakos, O. (1993). More basic forms of both human follicle-stimulating hormone and luteinizing hormone in serum at midcycle compared with the follicular or luteal phase. The Journal of Clinical Endocrinology and Metabolism, 76(4), 885-9.
Wide L, Bakos O. More Basic Forms of Both Human Follicle-stimulating Hormone and Luteinizing Hormone in Serum at Midcycle Compared With the Follicular or Luteal Phase. J Clin Endocrinol Metab. 1993;76(4):885-9. PubMed PMID: 8473400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - More basic forms of both human follicle-stimulating hormone and luteinizing hormone in serum at midcycle compared with the follicular or luteal phase. AU - Wide,L, AU - Bakos,O, PY - 1993/4/1/pubmed PY - 1993/4/1/medline PY - 1993/4/1/entrez SP - 885 EP - 9 JF - The Journal of clinical endocrinology and metabolism JO - J Clin Endocrinol Metab VL - 76 IS - 4 N2 - The purpose of this study was to compare the heterogeneity and median charge of FSH and LH in serum at different phases of the normal human menstrual cycle. Serum specimens were obtained during the follicular phase [9.0 +/- 1.9 (+/- SD) days before the midcycle], at the midcycle LH peak, and during the luteal phase (9.75 +/- 1.6 days after the midcycle) in 16 women with normal menstrual cycles. The 48 serum specimens were subjected to electrophoresis in 0.17% agarose suspension in 0.075 M veronal buffer at pH 8.6, using a column suitable for measuring the median charge of the isoforms of FSH and LH, expressed as median electrophoretic mobility. Four sera were also analyzed by use of a larger column, giving a high resolution. The FSH and LH activities were measured with a time-resolved sandwich fluoroimmunoassay. The number of isoforms of both FSH and LH in each serum specimen analyzed by electrophoresis with high resolution was between 20-30. The median charge of the isoforms of FSH was less negative (P < 0.001) at the midcycle than in the follicular or luteal phase in all 16 women. The same was found for LH in 14 of 15 women. The median charge of FSH or LH in the follicular phase was not significantly different from that in the luteal phase of the cycle. We conclude that at least 20-30 isoforms of both FSH and LH circulate in blood during the menstrual cycle. More basic isoforms of both hormones appear in serum at midcycle than in the follicular or luteal phase. The difference is most likely due to a selective secretion from the pituitary of more basic forms of FSH and LH at midcycle. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/8473400/More_basic_forms_of_both_human_follicle_stimulating_hormone_and_luteinizing_hormone_in_serum_at_midcycle_compared_with_the_follicular_or_luteal_phase_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem.76.4.8473400 DB - PRIME DP - Unbound Medicine ER -