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Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae.
Appl Environ Microbiol. 1993 Mar; 59(3):729-33.AE

Abstract

Pure nonhydrolyzed inulin was directly converted to ethanol in a simultaneous saccharification and fermentation process. An inulinase-hyperproducing mutant, Aspergillus niger 817, was grown in a submerged culture at 30 degrees C for 5 days. The inulin-digestive liquid culture (150 ml) was supplemented with 45 g of inulin, 0.45 g of (NH4)2SO4, and 0.15 g of KH2PO4. The medium (pH 5.0) was inoculated with an ethanol-tolerant strain, Saccharomyces cerevisiae 1200, and fermentation was conducted at 30 degrees C. An additional 20 g of inulin was added to the culture after 15 h of fermentation. S. cerevisiae 1200 utilized 99% of the 65 g of inulin during the fermentation, and produced 20.4 and 21.0% (vol/vol) ethanol from chicory and dahlia inulins, respectively, within 3 days of fermentation. The maximum volumetric productivities of ethanol were 6.2 and 6.0 g/liter/h for chicory and dahlia inulins, respectively. The conversion efficiency of inulin to ethanol was 83 to 84% of the theoretical ethanol yield.

Authors+Show Affiliations

Department of Agricultural Chemistry, Miyazaki University, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8481000

Citation

Ohta, K, et al. "Production of High Concentrations of Ethanol From Inulin By Simultaneous Saccharification and Fermentation Using Aspergillus Niger and Saccharomyces Cerevisiae." Applied and Environmental Microbiology, vol. 59, no. 3, 1993, pp. 729-33.
Ohta K, Hamada S, Nakamura T. Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae. Appl Environ Microbiol. 1993;59(3):729-33.
Ohta, K., Hamada, S., & Nakamura, T. (1993). Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae. Applied and Environmental Microbiology, 59(3), 729-33.
Ohta K, Hamada S, Nakamura T. Production of High Concentrations of Ethanol From Inulin By Simultaneous Saccharification and Fermentation Using Aspergillus Niger and Saccharomyces Cerevisiae. Appl Environ Microbiol. 1993;59(3):729-33. PubMed PMID: 8481000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae. AU - Ohta,K, AU - Hamada,S, AU - Nakamura,T, PY - 1993/3/1/pubmed PY - 1993/3/1/medline PY - 1993/3/1/entrez SP - 729 EP - 33 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 59 IS - 3 N2 - Pure nonhydrolyzed inulin was directly converted to ethanol in a simultaneous saccharification and fermentation process. An inulinase-hyperproducing mutant, Aspergillus niger 817, was grown in a submerged culture at 30 degrees C for 5 days. The inulin-digestive liquid culture (150 ml) was supplemented with 45 g of inulin, 0.45 g of (NH4)2SO4, and 0.15 g of KH2PO4. The medium (pH 5.0) was inoculated with an ethanol-tolerant strain, Saccharomyces cerevisiae 1200, and fermentation was conducted at 30 degrees C. An additional 20 g of inulin was added to the culture after 15 h of fermentation. S. cerevisiae 1200 utilized 99% of the 65 g of inulin during the fermentation, and produced 20.4 and 21.0% (vol/vol) ethanol from chicory and dahlia inulins, respectively, within 3 days of fermentation. The maximum volumetric productivities of ethanol were 6.2 and 6.0 g/liter/h for chicory and dahlia inulins, respectively. The conversion efficiency of inulin to ethanol was 83 to 84% of the theoretical ethanol yield. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/8481000/Production_of_high_concentrations_of_ethanol_from_inulin_by_simultaneous_saccharification_and_fermentation_using_Aspergillus_niger_and_Saccharomyces_cerevisiae_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=8481000 DB - PRIME DP - Unbound Medicine ER -