Tags

Type your tag names separated by a space and hit enter

Determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry.
Biol Mass Spectrom. 1993 Apr; 22(4):235-42.BM

Abstract

A sensitive and specific assay for the quantitative determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma specimens is described. Electron capture/negative ion chemical ionization gas chromatography/mass spectrometry is used to determine the extracted plasma concentrations of the three target compounds as their N-heptafluorobutyryl derivatives. Quantitation is performed by stable isotope dilution using d6-amphetamine and d6-methamphetamine as internal standards. Selected ion monitoring of the [M-HF]- ions of both the analytes and internal standards results in minimum quantifiable limits of 0.10 ng ml-1 for both amphetamine and methamphetamine and 0.25 ng ml-1 for desmethyldeprenyl. Excellent linearity (r = 0.998) up to at least 5.00 ng ml-1 is demonstrated.

Authors+Show Affiliations

Biomedical Mass Spectrometry Unit, McGill University, Montreal, Quebec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8481411

Citation

Reimer, M L., et al. "Determination of Amphetamine, Methamphetamine and Desmethyldeprenyl in Human Plasma By Gas Chromatography/negative Ion Chemical Ionization Mass Spectrometry." Biological Mass Spectrometry, vol. 22, no. 4, 1993, pp. 235-42.
Reimer ML, Mamer OA, Zavitsanos AP, et al. Determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry. Biol Mass Spectrom. 1993;22(4):235-42.
Reimer, M. L., Mamer, O. A., Zavitsanos, A. P., Siddiqui, A. W., & Dadgar, D. (1993). Determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry. Biological Mass Spectrometry, 22(4), 235-42.
Reimer ML, et al. Determination of Amphetamine, Methamphetamine and Desmethyldeprenyl in Human Plasma By Gas Chromatography/negative Ion Chemical Ionization Mass Spectrometry. Biol Mass Spectrom. 1993;22(4):235-42. PubMed PMID: 8481411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry. AU - Reimer,M L, AU - Mamer,O A, AU - Zavitsanos,A P, AU - Siddiqui,A W, AU - Dadgar,D, PY - 1993/4/1/pubmed PY - 1993/4/1/medline PY - 1993/4/1/entrez SP - 235 EP - 42 JF - Biological mass spectrometry JO - Biol. Mass Spectrom. VL - 22 IS - 4 N2 - A sensitive and specific assay for the quantitative determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma specimens is described. Electron capture/negative ion chemical ionization gas chromatography/mass spectrometry is used to determine the extracted plasma concentrations of the three target compounds as their N-heptafluorobutyryl derivatives. Quantitation is performed by stable isotope dilution using d6-amphetamine and d6-methamphetamine as internal standards. Selected ion monitoring of the [M-HF]- ions of both the analytes and internal standards results in minimum quantifiable limits of 0.10 ng ml-1 for both amphetamine and methamphetamine and 0.25 ng ml-1 for desmethyldeprenyl. Excellent linearity (r = 0.998) up to at least 5.00 ng ml-1 is demonstrated. SN - 1052-9306 UR - https://www.unboundmedicine.com/medline/citation/8481411/Determination_of_amphetamine_methamphetamine_and_desmethyldeprenyl_in_human_plasma_by_gas_chromatography/negative_ion_chemical_ionization_mass_spectrometry_ L2 - https://medlineplus.gov/methamphetamine.html DB - PRIME DP - Unbound Medicine ER -