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Fibroblast contraction of collagen gels requires activation of protein kinase C.
J Cell Physiol. 1993 May; 155(2):358-67.JC

Abstract

Fibroblasts stimulated to contract collagen gels with serum were completely inhibited by staurosporine, a broad spectrum kinase inhibitor. Further analysis demonstrated that staurosporine is potent (IC50 17 nM), rapid in onset, and completely reversible. Complete inhibition of gel contraction was also observed with calphostin C (IC50 48 nM), an inhibitor specific for protein kinase C (PKC). Similar effects were not observed with KT5926 or KT5720, inhibitors for myosin light chain kinase and cAMP-dependent kinases, respectively. These data suggested that serum-stimulated fibroblast contraction is dependent upon activation of PKC. This was also observed with fibroblast contraction stimulated with endothelin-1, platelet-derived growth factor, and transforming growth factor beta. PKC activated directly with low concentrations of phorbol ester was observed to stimulate fibroblast contraction of collagen gels, in some cases within 30 minutes of exposure.

Authors+Show Affiliations

Combined Program in Ophthalmology, University of Albama, Birmingham 35233.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8482728

Citation

Guidry, C. "Fibroblast Contraction of Collagen Gels Requires Activation of Protein Kinase C." Journal of Cellular Physiology, vol. 155, no. 2, 1993, pp. 358-67.
Guidry C. Fibroblast contraction of collagen gels requires activation of protein kinase C. J Cell Physiol. 1993;155(2):358-67.
Guidry, C. (1993). Fibroblast contraction of collagen gels requires activation of protein kinase C. Journal of Cellular Physiology, 155(2), 358-67.
Guidry C. Fibroblast Contraction of Collagen Gels Requires Activation of Protein Kinase C. J Cell Physiol. 1993;155(2):358-67. PubMed PMID: 8482728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fibroblast contraction of collagen gels requires activation of protein kinase C. A1 - Guidry,C, PY - 1993/5/1/pubmed PY - 1993/5/1/medline PY - 1993/5/1/entrez SP - 358 EP - 67 JF - Journal of cellular physiology JO - J Cell Physiol VL - 155 IS - 2 N2 - Fibroblasts stimulated to contract collagen gels with serum were completely inhibited by staurosporine, a broad spectrum kinase inhibitor. Further analysis demonstrated that staurosporine is potent (IC50 17 nM), rapid in onset, and completely reversible. Complete inhibition of gel contraction was also observed with calphostin C (IC50 48 nM), an inhibitor specific for protein kinase C (PKC). Similar effects were not observed with KT5926 or KT5720, inhibitors for myosin light chain kinase and cAMP-dependent kinases, respectively. These data suggested that serum-stimulated fibroblast contraction is dependent upon activation of PKC. This was also observed with fibroblast contraction stimulated with endothelin-1, platelet-derived growth factor, and transforming growth factor beta. PKC activated directly with low concentrations of phorbol ester was observed to stimulate fibroblast contraction of collagen gels, in some cases within 30 minutes of exposure. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/8482728/Fibroblast_contraction_of_collagen_gels_requires_activation_of_protein_kinase_C_ L2 - https://doi.org/10.1002/jcp.1041550217 DB - PRIME DP - Unbound Medicine ER -