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Primary structure and crystallization of orotate phosphoribosyltransferase from Salmonella typhimurium.
J Mol Biol. 1993 Apr 20; 230(4):1304-8.JM

Abstract

Orotate phosphoribosyltransferase (OPRTase; EC 2.4.2.10) catalyzes phosphoribosyl group transfer between alpha-D-5-phosphoribosyl-1-pyrophosphate and orotate to form orotidine-5'-monophosphate and pyrophosphate, the nucleotide-forming step in pyrimidine biosynthesis. It is one of ten PRTases that perform vital roles in de novo and salvage pathways for purine, pyrimidine and pyridine nucleotides. Although the PRTases are important drug targets, they are poorly understood mechanistically, and no three-dimensional structures exist. Here, we report the complete sequence of the Salmonella typhimurium pyrE gene and the deduced sequence of the OPRTase gene product. OPRTase forms tetragonal crystals from polyethylene glycol solutions; these crystals diffract to better than 2 A resolution, and are stable to radiation damage. The space group is P4(1)2(1)2 (or P4(3)2(1)2) with unit cell dimensions of a = b = 48.5 A, c = 210.5 A, and alpha = beta = gamma = 90 degrees. A crystalline form of the selenomethionine derivative of the protein is also reported.

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Authors+Show Affiliations

Scapin G
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
Sacchettini JC
No affiliation info available
Dessen A
No affiliation info available
Bhatia M
No affiliation info available
Grubmeyer C
No affiliation info available

MeSH

Amino Acid SequenceBase SequenceCrystallizationEscherichia coliMolecular Sequence DataOrotate PhosphoribosyltransferaseProtein ConformationRecombinant ProteinsSalmonella typhimuriumX-Ray Diffraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8487307

Citation

Scapin, G, et al. "Primary Structure and Crystallization of Orotate Phosphoribosyltransferase From Salmonella Typhimurium." Journal of Molecular Biology, vol. 230, no. 4, 1993, pp. 1304-8.
Scapin G, Sacchettini JC, Dessen A, et al. Primary structure and crystallization of orotate phosphoribosyltransferase from Salmonella typhimurium. J Mol Biol. 1993;230(4):1304-8.
Scapin, G., Sacchettini, J. C., Dessen, A., Bhatia, M., & Grubmeyer, C. (1993). Primary structure and crystallization of orotate phosphoribosyltransferase from Salmonella typhimurium. Journal of Molecular Biology, 230(4), 1304-8.
Scapin G, et al. Primary Structure and Crystallization of Orotate Phosphoribosyltransferase From Salmonella Typhimurium. J Mol Biol. 1993 Apr 20;230(4):1304-8. PubMed PMID: 8487307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Primary structure and crystallization of orotate phosphoribosyltransferase from Salmonella typhimurium. AU - Scapin,G, AU - Sacchettini,J C, AU - Dessen,A, AU - Bhatia,M, AU - Grubmeyer,C, PY - 1993/4/20/pubmed PY - 1993/4/20/medline PY - 1993/4/20/entrez SP - 1304 EP - 8 JF - Journal of molecular biology JO - J Mol Biol VL - 230 IS - 4 N2 - Orotate phosphoribosyltransferase (OPRTase; EC 2.4.2.10) catalyzes phosphoribosyl group transfer between alpha-D-5-phosphoribosyl-1-pyrophosphate and orotate to form orotidine-5'-monophosphate and pyrophosphate, the nucleotide-forming step in pyrimidine biosynthesis. It is one of ten PRTases that perform vital roles in de novo and salvage pathways for purine, pyrimidine and pyridine nucleotides. Although the PRTases are important drug targets, they are poorly understood mechanistically, and no three-dimensional structures exist. Here, we report the complete sequence of the Salmonella typhimurium pyrE gene and the deduced sequence of the OPRTase gene product. OPRTase forms tetragonal crystals from polyethylene glycol solutions; these crystals diffract to better than 2 A resolution, and are stable to radiation damage. The space group is P4(1)2(1)2 (or P4(3)2(1)2) with unit cell dimensions of a = b = 48.5 A, c = 210.5 A, and alpha = beta = gamma = 90 degrees. A crystalline form of the selenomethionine derivative of the protein is also reported. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/8487307/Primary_structure_and_crystallization_of_orotate_phosphoribosyltransferase_from_Salmonella_typhimurium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(83)71244-1 DB - PRIME DP - Unbound Medicine ER -