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Age-related changes in delta 6 and delta 5 desaturase activities in rat liver microsomes.
Lipids. 1993 Apr; 28(4):291-7.L

Abstract

Age-related changes in delta 6 desaturation of [1-14C]alpha-linolenic acid and [1-14C]linoleic acid and in delta 5 desaturation of [2-14C]dihomo-gamma-linolenic acid were studied in liver microsomes from Wistar male rats at various ages ranging from 1.5 to 24 mon. Desaturase activities were expressed both as specific activity of liver microsomes and as the capacity of whole liver to desaturate by taking into account the total amount of liver microsomal protein. delta 6 Desaturation of alpha-linolenic acid increased from 1.5 to 3 mon and then decreased linearly up to 24 mon to reach the same desaturation capacity of liver measured at 1.5 mon. The capacity of liver to desaturate linoleic acid increased up to 6 mon and then remained constant, whereas microsomal specific activity was equal at 1.5 and 24 mon of age. The capacity of liver to convert dihomo-gamma-linolenic acid to arachidonic acid by delta 5 desaturation decreased markedly from 1.5 to 3 mon. It then increased to reach, at 24 mon, the same level as that observed at 1.5 mon. Age-related changes in the fatty acid composition of liver microsomal phospholipids at the seven time points studied and of erythrocyte lipids at 1.5 and 24 mon were consistent with the variations in desaturation capacity of liver. In particular, arachidonic acid content in old rats was slightly higher than in young rats whereas contents in linoleic and docosahexaenoic acids varied little throughout the life span.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Unité de Recherche de Nutrition Cellulaire et Métabolique, Université de Bourgogne, Dijon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8487621

Citation

Maniongui, C, et al. "Age-related Changes in Delta 6 and Delta 5 Desaturase Activities in Rat Liver Microsomes." Lipids, vol. 28, no. 4, 1993, pp. 291-7.
Maniongui C, Blond JP, Ulmann L, et al. Age-related changes in delta 6 and delta 5 desaturase activities in rat liver microsomes. Lipids. 1993;28(4):291-7.
Maniongui, C., Blond, J. P., Ulmann, L., Durand, G., Poisson, J. P., & Bézard, J. (1993). Age-related changes in delta 6 and delta 5 desaturase activities in rat liver microsomes. Lipids, 28(4), 291-7.
Maniongui C, et al. Age-related Changes in Delta 6 and Delta 5 Desaturase Activities in Rat Liver Microsomes. Lipids. 1993;28(4):291-7. PubMed PMID: 8487621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Age-related changes in delta 6 and delta 5 desaturase activities in rat liver microsomes. AU - Maniongui,C, AU - Blond,J P, AU - Ulmann,L, AU - Durand,G, AU - Poisson,J P, AU - Bézard,J, PY - 1993/4/1/pubmed PY - 1993/4/1/medline PY - 1993/4/1/entrez SP - 291 EP - 7 JF - Lipids JO - Lipids VL - 28 IS - 4 N2 - Age-related changes in delta 6 desaturation of [1-14C]alpha-linolenic acid and [1-14C]linoleic acid and in delta 5 desaturation of [2-14C]dihomo-gamma-linolenic acid were studied in liver microsomes from Wistar male rats at various ages ranging from 1.5 to 24 mon. Desaturase activities were expressed both as specific activity of liver microsomes and as the capacity of whole liver to desaturate by taking into account the total amount of liver microsomal protein. delta 6 Desaturation of alpha-linolenic acid increased from 1.5 to 3 mon and then decreased linearly up to 24 mon to reach the same desaturation capacity of liver measured at 1.5 mon. The capacity of liver to desaturate linoleic acid increased up to 6 mon and then remained constant, whereas microsomal specific activity was equal at 1.5 and 24 mon of age. The capacity of liver to convert dihomo-gamma-linolenic acid to arachidonic acid by delta 5 desaturation decreased markedly from 1.5 to 3 mon. It then increased to reach, at 24 mon, the same level as that observed at 1.5 mon. Age-related changes in the fatty acid composition of liver microsomal phospholipids at the seven time points studied and of erythrocyte lipids at 1.5 and 24 mon were consistent with the variations in desaturation capacity of liver. In particular, arachidonic acid content in old rats was slightly higher than in young rats whereas contents in linoleic and docosahexaenoic acids varied little throughout the life span.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0024-4201 UR - https://www.unboundmedicine.com/medline/citation/8487621/Age_related_changes_in_delta_6_and_delta_5_desaturase_activities_in_rat_liver_microsomes_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0024-4201&date=1993&volume=28&issue=4&spage=291 DB - PRIME DP - Unbound Medicine ER -