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Detection of ras gene mutations in pancreatic juice and peripheral blood of patients with pancreatic adenocarcinoma.
Cancer Res. 1993 Jun 01; 53(11):2472-4.CR

Abstract

Pancreatic adenocarcinomas are known to have a high incidence of K-ras gene mutations. Differential diagnosis of pancreatic cancer and chronic pancreatitis sometimes presents a clinical dilemma. We recently developed a highly sensitive and specific polymerase chain reaction capable of detecting 3-30 copies of mutant K-ras genes harboring codon 12 single base changes in the presence of 300,000 normal copies. Mutant ras genes were detected in DNA purified from pancreatic juice from all 6 cases of pancreatic adenocarcinoma and 1 case of intraductal papillary neoplasms of the pancreas. In 2 of 6 other cases with pancreatic adenocarcinoma, circulating metastatic cells were detected in DNA purified from peripheral blood. Activated ras genes were not found in pancreatic juice of three control cases (chronic pancreatitis and choledocholithiasis) or in the peripheral blood of two patients with insulinomas. Notable conclusions of this study are that there can be significant levels of shed tumor cells in peripheral blood and an even higher number in pancreatic juice. In addition, two different K-ras mutations were found in some patients.

Authors+Show Affiliations

Second Department of Medicine, Faculty of Medicine, University of Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8495407

Citation

Tada, M, et al. "Detection of Ras Gene Mutations in Pancreatic Juice and Peripheral Blood of Patients With Pancreatic Adenocarcinoma." Cancer Research, vol. 53, no. 11, 1993, pp. 2472-4.
Tada M, Omata M, Kawai S, et al. Detection of ras gene mutations in pancreatic juice and peripheral blood of patients with pancreatic adenocarcinoma. Cancer Res. 1993;53(11):2472-4.
Tada, M., Omata, M., Kawai, S., Saisho, H., Ohto, M., Saiki, R. K., & Sninsky, J. J. (1993). Detection of ras gene mutations in pancreatic juice and peripheral blood of patients with pancreatic adenocarcinoma. Cancer Research, 53(11), 2472-4.
Tada M, et al. Detection of Ras Gene Mutations in Pancreatic Juice and Peripheral Blood of Patients With Pancreatic Adenocarcinoma. Cancer Res. 1993 Jun 1;53(11):2472-4. PubMed PMID: 8495407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of ras gene mutations in pancreatic juice and peripheral blood of patients with pancreatic adenocarcinoma. AU - Tada,M, AU - Omata,M, AU - Kawai,S, AU - Saisho,H, AU - Ohto,M, AU - Saiki,R K, AU - Sninsky,J J, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 2472 EP - 4 JF - Cancer research JO - Cancer Res VL - 53 IS - 11 N2 - Pancreatic adenocarcinomas are known to have a high incidence of K-ras gene mutations. Differential diagnosis of pancreatic cancer and chronic pancreatitis sometimes presents a clinical dilemma. We recently developed a highly sensitive and specific polymerase chain reaction capable of detecting 3-30 copies of mutant K-ras genes harboring codon 12 single base changes in the presence of 300,000 normal copies. Mutant ras genes were detected in DNA purified from pancreatic juice from all 6 cases of pancreatic adenocarcinoma and 1 case of intraductal papillary neoplasms of the pancreas. In 2 of 6 other cases with pancreatic adenocarcinoma, circulating metastatic cells were detected in DNA purified from peripheral blood. Activated ras genes were not found in pancreatic juice of three control cases (chronic pancreatitis and choledocholithiasis) or in the peripheral blood of two patients with insulinomas. Notable conclusions of this study are that there can be significant levels of shed tumor cells in peripheral blood and an even higher number in pancreatic juice. In addition, two different K-ras mutations were found in some patients. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/8495407/Detection_of_ras_gene_mutations_in_pancreatic_juice_and_peripheral_blood_of_patients_with_pancreatic_adenocarcinoma_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=8495407 DB - PRIME DP - Unbound Medicine ER -