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Humoral regulation of vitamin B12 absorption by pregnant mouse small intestine.
Gastroenterology. 1977 May; 72(5 Pt 1):881-8.G

Abstract

In vivo experiments in which pregnant mice were fed an oral dose of 40 ng of [57Co]B12 demonstrated that there was a progressive increase in B12 absorption in the 3rd week of pregnancy. The absorptive mechanism resembled that in nonpregnant animals on the grounds that the mechanism was saturable, and presumably intrinsic factor (IF) dependent, and that it was localized to the ileum. In vitro studies of IF-57Co B12 binding to ileal homogenates confirmed the requirement for IF. Studies in late pregnancy showed that IF-[57Co]B12 binding to ileal homogenates, expressed as nanograms of IF-[57Co]B12 bound per milligram of protein, was twice that of nonpregnant animals. When ileal homogenates were prepared from mice on days 20 to 21 of gestation, IF-[57Co]B12 binding fell to nonpregnant levels within 2 to 4 hr of hysterectomy. Hourly injections of serum from mice in late pregnancy, or from a female at the 37th week of gestation, maintained IF-B12 binding at levels reached during pregnancy, whereas nonpregnant serum was ineffective.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

849818

Citation

Brown, J, et al. "Humoral Regulation of Vitamin B12 Absorption By Pregnant Mouse Small Intestine." Gastroenterology, vol. 72, no. 5 Pt 1, 1977, pp. 881-8.
Brown J, Robertson J, Gallagher N. Humoral regulation of vitamin B12 absorption by pregnant mouse small intestine. Gastroenterology. 1977;72(5 Pt 1):881-8.
Brown, J., Robertson, J., & Gallagher, N. (1977). Humoral regulation of vitamin B12 absorption by pregnant mouse small intestine. Gastroenterology, 72(5 Pt 1), 881-8.
Brown J, Robertson J, Gallagher N. Humoral Regulation of Vitamin B12 Absorption By Pregnant Mouse Small Intestine. Gastroenterology. 1977;72(5 Pt 1):881-8. PubMed PMID: 849818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Humoral regulation of vitamin B12 absorption by pregnant mouse small intestine. AU - Brown,J, AU - Robertson,J, AU - Gallagher,N, PY - 1977/5/1/pubmed PY - 1977/5/1/medline PY - 1977/5/1/entrez SP - 881 EP - 8 JF - Gastroenterology JO - Gastroenterology VL - 72 IS - 5 Pt 1 N2 - In vivo experiments in which pregnant mice were fed an oral dose of 40 ng of [57Co]B12 demonstrated that there was a progressive increase in B12 absorption in the 3rd week of pregnancy. The absorptive mechanism resembled that in nonpregnant animals on the grounds that the mechanism was saturable, and presumably intrinsic factor (IF) dependent, and that it was localized to the ileum. In vitro studies of IF-57Co B12 binding to ileal homogenates confirmed the requirement for IF. Studies in late pregnancy showed that IF-[57Co]B12 binding to ileal homogenates, expressed as nanograms of IF-[57Co]B12 bound per milligram of protein, was twice that of nonpregnant animals. When ileal homogenates were prepared from mice on days 20 to 21 of gestation, IF-[57Co]B12 binding fell to nonpregnant levels within 2 to 4 hr of hysterectomy. Hourly injections of serum from mice in late pregnancy, or from a female at the 37th week of gestation, maintained IF-B12 binding at levels reached during pregnancy, whereas nonpregnant serum was ineffective. SN - 0016-5085 UR - https://www.unboundmedicine.com/medline/citation/849818/Humoral_regulation_of_vitamin_B12_absorption_by_pregnant_mouse_small_intestine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016508577001450 DB - PRIME DP - Unbound Medicine ER -