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Lectin-detectable glycoconjugate profile of the tracheal secretions and epithelial glycocalyx in sheep. Effect of muscarinic stimulation.
Am Rev Respir Dis. 1993 Jun; 147(6 Pt 1):1550-6.AR

Abstract

Reflex mucus secretion in the airways serves a defense function that includes the binding of bacteria to mucus glycoconjugates thereby preventing bacterial adherence to the epithelium. We therefore compared the lectin-detectable glycoconjugate profile of the epithelial glycocalyx and luminal secretions under baseline conditions and after muscarinic receptor stimulation in the sheep trachea. The sheep were intubated with a double-balloon nasotracheal tube to create a tracheal chamber for collection of secretions. After an initial lavage of the chamber to clear it of secretions, the sheep received an intravenous injection of normal saline, 0.5 mg/kg pilocarpine, or 0.5 mg/kg pilocarpine after pretreatment with 0.2 mg/kg atropine. Tracheal lavage was repeated 2 h later, and the sheep were then killed. An enzyme-linked lectin assay and lectin histochemistry were used to characterize glycoconjugate residues in tracheal secretions and in the apical epithelial glycocalyx, respectively. Eight different lectins were used to detect N-acetyl galactosamine, alpha-galactose, alpha-galactose-N-acetyl galactosamine, beta-galactose, beta-galactose-N-acetyl galactosamine, alpha-fucose, alpha-glucose, alpha-mannose and alpha-(2-3)sialyl residues. After normal saline, reactivity was present for all glycoconjugates in secretions and in the glycocalyx.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Pulmonary Division, University of Miami, Mount Sinai Medical Center, Miami Beach, Florida 33140.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8503568

Citation

Mariassy, A T., et al. "Lectin-detectable Glycoconjugate Profile of the Tracheal Secretions and Epithelial Glycocalyx in Sheep. Effect of Muscarinic Stimulation." The American Review of Respiratory Disease, vol. 147, no. 6 Pt 1, 1993, pp. 1550-6.
Mariassy AT, Toussaint KT, Guldimann P, et al. Lectin-detectable glycoconjugate profile of the tracheal secretions and epithelial glycocalyx in sheep. Effect of muscarinic stimulation. Am Rev Respir Dis. 1993;147(6 Pt 1):1550-6.
Mariassy, A. T., Toussaint, K. T., Guldimann, P., Abraham, W. M., & Wanner, A. (1993). Lectin-detectable glycoconjugate profile of the tracheal secretions and epithelial glycocalyx in sheep. Effect of muscarinic stimulation. The American Review of Respiratory Disease, 147(6 Pt 1), 1550-6.
Mariassy AT, et al. Lectin-detectable Glycoconjugate Profile of the Tracheal Secretions and Epithelial Glycocalyx in Sheep. Effect of Muscarinic Stimulation. Am Rev Respir Dis. 1993;147(6 Pt 1):1550-6. PubMed PMID: 8503568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lectin-detectable glycoconjugate profile of the tracheal secretions and epithelial glycocalyx in sheep. Effect of muscarinic stimulation. AU - Mariassy,A T, AU - Toussaint,K T, AU - Guldimann,P, AU - Abraham,W M, AU - Wanner,A, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 1550 EP - 6 JF - The American review of respiratory disease JO - Am. Rev. Respir. Dis. VL - 147 IS - 6 Pt 1 N2 - Reflex mucus secretion in the airways serves a defense function that includes the binding of bacteria to mucus glycoconjugates thereby preventing bacterial adherence to the epithelium. We therefore compared the lectin-detectable glycoconjugate profile of the epithelial glycocalyx and luminal secretions under baseline conditions and after muscarinic receptor stimulation in the sheep trachea. The sheep were intubated with a double-balloon nasotracheal tube to create a tracheal chamber for collection of secretions. After an initial lavage of the chamber to clear it of secretions, the sheep received an intravenous injection of normal saline, 0.5 mg/kg pilocarpine, or 0.5 mg/kg pilocarpine after pretreatment with 0.2 mg/kg atropine. Tracheal lavage was repeated 2 h later, and the sheep were then killed. An enzyme-linked lectin assay and lectin histochemistry were used to characterize glycoconjugate residues in tracheal secretions and in the apical epithelial glycocalyx, respectively. Eight different lectins were used to detect N-acetyl galactosamine, alpha-galactose, alpha-galactose-N-acetyl galactosamine, beta-galactose, beta-galactose-N-acetyl galactosamine, alpha-fucose, alpha-glucose, alpha-mannose and alpha-(2-3)sialyl residues. After normal saline, reactivity was present for all glycoconjugates in secretions and in the glycocalyx.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0003-0805 UR - https://www.unboundmedicine.com/medline/citation/8503568/Lectin_detectable_glycoconjugate_profile_of_the_tracheal_secretions_and_epithelial_glycocalyx_in_sheep__Effect_of_muscarinic_stimulation_ L2 - http://www.atsjournals.org/doi/full/10.1164/ajrccm/147.6_Pt_1.1550?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -