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[Detection antigen of Rickettsia tsutsugamushi by using monoclonal antibody].
Zhonghua Liu Xing Bing Xue Za Zhi. 1993 Feb; 14(1):49-51.ZL

Abstract

Dot-ELISA was used with HRP-labelled monoclonal antibodies Kp3 (IgGi) and Kp4-6 (IgM) against antigen of the Karp strain of Rickettsia tsutsugamushi to detect the antigen of R. tsutsugamushi. The positive rate of sera of 56 acute scrub typhus patients and 37 wild rats caught from the countryside of the endemic area as well as 29 batches of chiggers collected from the wild rats were 92.9%, 100% and 89.7%, respectively. No cross reaction was found with antigens of other rickettsia groups such as R. mooseri, R. prowazeki, R.burneti and R. rickettsia. In patients suffering from acute scrub typhus the antigen of R. tsutsugamushi could be detected earlier in the course of illness than the antibody. This method is very sensitive that an amount of antigen of 6.7ng/microliters can be detected. It is easy to perform and the results can be read by naked eye.

Authors+Show Affiliations

Zhang H
Beijing Tropical Medicine Research Institute.

MeSH

AnimalsAntibodies, MonoclonalAntigens, BacterialEnzyme-Linked Immunosorbent AssayHumansMuridaeOrientia tsutsugamushiScrub TyphusTrombiculidae

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

8504454

Citation

Zhang, H. "[Detection Antigen of Rickettsia Tsutsugamushi By Using Monoclonal Antibody]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 14, no. 1, 1993, pp. 49-51.
Zhang H. [Detection antigen of Rickettsia tsutsugamushi by using monoclonal antibody]. Zhonghua Liu Xing Bing Xue Za Zhi. 1993;14(1):49-51.
Zhang, H. (1993). [Detection antigen of Rickettsia tsutsugamushi by using monoclonal antibody]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 14(1), 49-51.
Zhang H. [Detection Antigen of Rickettsia Tsutsugamushi By Using Monoclonal Antibody]. Zhonghua Liu Xing Bing Xue Za Zhi. 1993;14(1):49-51. PubMed PMID: 8504454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection antigen of Rickettsia tsutsugamushi by using monoclonal antibody]. A1 - Zhang,H, PY - 1993/2/1/pubmed PY - 1993/2/1/medline PY - 1993/2/1/entrez SP - 49 EP - 51 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 14 IS - 1 N2 - Dot-ELISA was used with HRP-labelled monoclonal antibodies Kp3 (IgGi) and Kp4-6 (IgM) against antigen of the Karp strain of Rickettsia tsutsugamushi to detect the antigen of R. tsutsugamushi. The positive rate of sera of 56 acute scrub typhus patients and 37 wild rats caught from the countryside of the endemic area as well as 29 batches of chiggers collected from the wild rats were 92.9%, 100% and 89.7%, respectively. No cross reaction was found with antigens of other rickettsia groups such as R. mooseri, R. prowazeki, R.burneti and R. rickettsia. In patients suffering from acute scrub typhus the antigen of R. tsutsugamushi could be detected earlier in the course of illness than the antibody. This method is very sensitive that an amount of antigen of 6.7ng/microliters can be detected. It is easy to perform and the results can be read by naked eye. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/8504454/[Detection_antigen_of_Rickettsia_tsutsugamushi_by_using_monoclonal_antibody]_ DB - PRIME DP - Unbound Medicine ER -