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Immunological studies using monoclonal antibodies for detection of low dalton marine toxins.
Food Addit Contam. 1993 Jan-Feb; 10(1):83-95.FA

Abstract

The general methodology employed in the development of an immunological assay system is initially described. These include: (a) the methods and reagents used for coupling low dalton epitopes; (b) selection of appropriate immunogenic carriers; (c) mode of administration and choice of animal; and (d) the method of assessing the antibody produced. The background and methodology, using ciguatoxin as a model, are presented. The stick enzyme immunoassay (S-EIA) has proven of value to evaluate implicated fish for verification of ciguatera poisoning and for screening of fish associated with ciguatera prior to consumption. No false negatives have occurred with the stick enzyme immunoassay system to date.

Authors+Show Affiliations

Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu 96822.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

8504877

Citation

Hokama, Y. "Immunological Studies Using Monoclonal Antibodies for Detection of Low Dalton Marine Toxins." Food Additives and Contaminants, vol. 10, no. 1, 1993, pp. 83-95.
Hokama Y. Immunological studies using monoclonal antibodies for detection of low dalton marine toxins. Food Addit Contam. 1993;10(1):83-95.
Hokama, Y. (1993). Immunological studies using monoclonal antibodies for detection of low dalton marine toxins. Food Additives and Contaminants, 10(1), 83-95.
Hokama Y. Immunological Studies Using Monoclonal Antibodies for Detection of Low Dalton Marine Toxins. Food Addit Contam. 1993 Jan-Feb;10(1):83-95. PubMed PMID: 8504877.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunological studies using monoclonal antibodies for detection of low dalton marine toxins. A1 - Hokama,Y, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 83 EP - 95 JF - Food additives and contaminants JO - Food Addit Contam VL - 10 IS - 1 N2 - The general methodology employed in the development of an immunological assay system is initially described. These include: (a) the methods and reagents used for coupling low dalton epitopes; (b) selection of appropriate immunogenic carriers; (c) mode of administration and choice of animal; and (d) the method of assessing the antibody produced. The background and methodology, using ciguatoxin as a model, are presented. The stick enzyme immunoassay (S-EIA) has proven of value to evaluate implicated fish for verification of ciguatera poisoning and for screening of fish associated with ciguatera prior to consumption. No false negatives have occurred with the stick enzyme immunoassay system to date. SN - 0265-203X UR - https://www.unboundmedicine.com/medline/citation/8504877/Immunological_studies_using_monoclonal_antibodies_for_detection_of_low_dalton_marine_toxins_ L2 - https://medlineplus.gov/foodborneillness.html DB - PRIME DP - Unbound Medicine ER -