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Exogenous lipids influence permeability barrier recovery in acetone-treated murine skin.
Arch Dermatol. 1993 Jun; 129(6):728-38.AD

Abstract

BACKGROUND AND DESIGN

When the epidermal permeability barrier is perturbed with acetone, transcutaneous water loss returns to normal over 24 hours in parallel with the reappearance of stratum corneum lipids, derived from secreted lamellar bodies and accelerated lipid synthesis. Prior studies have demonstrated a separate requirement for cholesterol, fatty acid, and ceramide synthesis for barrier homeostasis, but the requirement for these lipids individually or together for barrier function is not known. We therefore applied these lipids alone or in various mixtures to acetone-treated hairless mouse skin and assessed barrier recovery.

RESULTS

Ceramide and fatty acid alone, and their complex derivatives (cholesterol esters and cerebrosides), and two-component mixtures of fatty acid plus ceramide, cholesterol plus fatty acid, or cholesterol plus ceramide delayed barrier recovery. In contrast, complete mixtures of ceramide, fatty acid, and cholesterol allowed normal barrier recovery. Moreover, fluorescent-labeled cholesterol, fatty acid, and ceramide rapidly traversed the stratum corneum with uptake into the epidermal nucleated layers. Finally, incomplete, but not complete mixtures produce abnormal lamellar bodies, leading to abnormal stratum corneum intercellular membrane bilayers.

CONCLUSIONS

(1) Topical applications of individual lipids or incomplete mixtures of lipids interfere with barrier recovery, while complete mixtures of cholesterol, fatty acid, and ceramide allow normal barrier repair; and (2) incomplete mixtures of topical lipids appear to inhibit barrier recovery at the level of the lamellar body resulting in abnormal intercellular membrane structures in the stratum corneum, abnormalities that do not occur when a complete lipid mixture is provided.

Authors+Show Affiliations

Dermatology Service, Veterans Affairs Medical Center, San Francisco, CA 94121.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8507075

Citation

Man, M Q., et al. "Exogenous Lipids Influence Permeability Barrier Recovery in Acetone-treated Murine Skin." Archives of Dermatology, vol. 129, no. 6, 1993, pp. 728-38.
Man MQ, Feingold KR, Elias PM. Exogenous lipids influence permeability barrier recovery in acetone-treated murine skin. Arch Dermatol. 1993;129(6):728-38.
Man, M. Q., Feingold, K. R., & Elias, P. M. (1993). Exogenous lipids influence permeability barrier recovery in acetone-treated murine skin. Archives of Dermatology, 129(6), 728-38.
Man MQ, Feingold KR, Elias PM. Exogenous Lipids Influence Permeability Barrier Recovery in Acetone-treated Murine Skin. Arch Dermatol. 1993;129(6):728-38. PubMed PMID: 8507075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous lipids influence permeability barrier recovery in acetone-treated murine skin. AU - Man,M Q, AU - Feingold,K R, AU - Elias,P M, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 728 EP - 38 JF - Archives of dermatology JO - Arch Dermatol VL - 129 IS - 6 N2 - BACKGROUND AND DESIGN: When the epidermal permeability barrier is perturbed with acetone, transcutaneous water loss returns to normal over 24 hours in parallel with the reappearance of stratum corneum lipids, derived from secreted lamellar bodies and accelerated lipid synthesis. Prior studies have demonstrated a separate requirement for cholesterol, fatty acid, and ceramide synthesis for barrier homeostasis, but the requirement for these lipids individually or together for barrier function is not known. We therefore applied these lipids alone or in various mixtures to acetone-treated hairless mouse skin and assessed barrier recovery. RESULTS: Ceramide and fatty acid alone, and their complex derivatives (cholesterol esters and cerebrosides), and two-component mixtures of fatty acid plus ceramide, cholesterol plus fatty acid, or cholesterol plus ceramide delayed barrier recovery. In contrast, complete mixtures of ceramide, fatty acid, and cholesterol allowed normal barrier recovery. Moreover, fluorescent-labeled cholesterol, fatty acid, and ceramide rapidly traversed the stratum corneum with uptake into the epidermal nucleated layers. Finally, incomplete, but not complete mixtures produce abnormal lamellar bodies, leading to abnormal stratum corneum intercellular membrane bilayers. CONCLUSIONS: (1) Topical applications of individual lipids or incomplete mixtures of lipids interfere with barrier recovery, while complete mixtures of cholesterol, fatty acid, and ceramide allow normal barrier repair; and (2) incomplete mixtures of topical lipids appear to inhibit barrier recovery at the level of the lamellar body resulting in abnormal intercellular membrane structures in the stratum corneum, abnormalities that do not occur when a complete lipid mixture is provided. SN - 0003-987X UR - https://www.unboundmedicine.com/medline/citation/8507075/Exogenous_lipids_influence_permeability_barrier_recovery_in_acetone_treated_murine_skin_ L2 - https://jamanetwork.com/journals/jamadermatology/fullarticle/vol/129/pg/728 DB - PRIME DP - Unbound Medicine ER -