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[Distribution of oligonucleotide derivatives and their stability in murine tissues].
Biokhimiia. 1993 Apr; 58(4):590-8.B

Abstract

Distribution and stability of benzyl-[5'-32P] phosphoramides of oligonucleotides and their phosphorothioate analogs were investigated. Oligonucleotide derivatives are distributed among all murine organs, the maximal concentration being observed in the liver and kidney, and minimal in the brain. Intravenous and intraperitoneal injections resulted in a faster distribution of oligonucleotides among the tissues than subcutaneous injections. Hydrolysis rate varied from tissue to tissue, and in 30 min after injection 5 to 50% of intact oligonucleotides were found. Hydrolysis did not depend on the injected dose in the range of 0.15-150 nmol per mouse. The 3'-terminal cholesterol groups protected the oligonucleotides considerably. In the blood stream and pancreas phosphorothioate oligonucleotides showed much higher stability than their phosphodiester counterparts. A protein interacting specifically with the oligonucleotides was discovered in the liver, kidney and pancreas.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

8507735

Citation

Karamyshev, V N., et al. "[Distribution of Oligonucleotide Derivatives and Their Stability in Murine Tissues]." Biokhimiia (Moscow, Russia), vol. 58, no. 4, 1993, pp. 590-8.
Karamyshev VN, Vlasov VV, Zon D, et al. [Distribution of oligonucleotide derivatives and their stability in murine tissues]. Biokhimiia. 1993;58(4):590-8.
Karamyshev, V. N., Vlasov, V. V., Zon, D., Ivanova, E. M., & Iakubov, L. A. (1993). [Distribution of oligonucleotide derivatives and their stability in murine tissues]. Biokhimiia (Moscow, Russia), 58(4), 590-8.
Karamyshev VN, et al. [Distribution of Oligonucleotide Derivatives and Their Stability in Murine Tissues]. Biokhimiia. 1993;58(4):590-8. PubMed PMID: 8507735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Distribution of oligonucleotide derivatives and their stability in murine tissues]. AU - Karamyshev,V N, AU - Vlasov,V V, AU - Zon,D, AU - Ivanova,E M, AU - Iakubov,L A, PY - 1993/4/1/pubmed PY - 1993/4/1/medline PY - 1993/4/1/entrez SP - 590 EP - 8 JF - Biokhimiia (Moscow, Russia) JO - Biokhimiia VL - 58 IS - 4 N2 - Distribution and stability of benzyl-[5'-32P] phosphoramides of oligonucleotides and their phosphorothioate analogs were investigated. Oligonucleotide derivatives are distributed among all murine organs, the maximal concentration being observed in the liver and kidney, and minimal in the brain. Intravenous and intraperitoneal injections resulted in a faster distribution of oligonucleotides among the tissues than subcutaneous injections. Hydrolysis rate varied from tissue to tissue, and in 30 min after injection 5 to 50% of intact oligonucleotides were found. Hydrolysis did not depend on the injected dose in the range of 0.15-150 nmol per mouse. The 3'-terminal cholesterol groups protected the oligonucleotides considerably. In the blood stream and pancreas phosphorothioate oligonucleotides showed much higher stability than their phosphodiester counterparts. A protein interacting specifically with the oligonucleotides was discovered in the liver, kidney and pancreas. SN - 0320-9725 UR - https://www.unboundmedicine.com/medline/citation/8507735/[Distribution_of_oligonucleotide_derivatives_and_their_stability_in_murine_tissues]_ DB - PRIME DP - Unbound Medicine ER -