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Properties of a phosphatidylcholine derivative of diphenyl hexatriene (DPH-PC) in lymphocyte membranes. A comparison with DPH and the cationic derivative TMA-DPH using static and dynamic fluorescence.
Membr Biochem. 1993 Jan-Mar; 10(1):17-27.MB

Abstract

Using static and dynamic fluorescence we studied the fluorescence properties of a phosphatidylcholine analog of 1,6-diphenyl-1,3,5-hexatriene (DPH-PC) incorporated in lymphocyte plasma membranes with respect to DPH and its cationic derivative 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH), in order to study if phospholipid derivatives of DPH may be used to investigate structural and physicochemical properties of specific membrane lipid domains. DPH-PC and TMA-DPH showed similar fluorescence polarization values that were significantly higher with respect to DPH, suggesting a localization of the fluorescent portion of DPH-PC in a more ordered region of the membrane which was probably due to the elecrostatic interactions between phospholipid head-groups. The localization of the fluorescent moiety of DPH-PC near the membrane surface was also supported by the study of the fluorescence decay of the three probes using frequency-domain fluorometry. The main lifetime component of DPH-PC was rather similar to that of TMA-DPH (6.74 versus 6.24, ns) but considerably lower with respect to DPH (10.52 ns), in agreement with data obtained from exponential analysis. In lymphocyte membranes obtained from concanavalin A treated cells, a significant decrease of fluorescence polarization has been shown with DPH and its phosphatidylcholine derivative, but not with TMA-DPH. In liposomes obtained from total lipids extracted from lymphocyte membranes, a decrease of fluorescence polarization has been observed only with DPH. Our results suggest that DPH-PC localizes the fluorescent portion of its molecule in membrane microenvironments of different properties with respect to those probed by DPH and TMA-DPH. The use of DPH-phospholipid derivatives and other DPH-probes may represent an useful tool to study plasma membrane heterogeneity in biological membranes.

Authors+Show Affiliations

Institute of Biochemistry, Faculty of Medicine, University of Ancona, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8510559

Citation

Ferretti, G, et al. "Properties of a Phosphatidylcholine Derivative of Diphenyl Hexatriene (DPH-PC) in Lymphocyte Membranes. a Comparison With DPH and the Cationic Derivative TMA-DPH Using Static and Dynamic Fluorescence." Membrane Biochemistry, vol. 10, no. 1, 1993, pp. 17-27.
Ferretti G, Tangorra A, Zolese G, et al. Properties of a phosphatidylcholine derivative of diphenyl hexatriene (DPH-PC) in lymphocyte membranes. A comparison with DPH and the cationic derivative TMA-DPH using static and dynamic fluorescence. Membr Biochem. 1993;10(1):17-27.
Ferretti, G., Tangorra, A., Zolese, G., & Curatola, G. (1993). Properties of a phosphatidylcholine derivative of diphenyl hexatriene (DPH-PC) in lymphocyte membranes. A comparison with DPH and the cationic derivative TMA-DPH using static and dynamic fluorescence. Membrane Biochemistry, 10(1), 17-27.
Ferretti G, et al. Properties of a Phosphatidylcholine Derivative of Diphenyl Hexatriene (DPH-PC) in Lymphocyte Membranes. a Comparison With DPH and the Cationic Derivative TMA-DPH Using Static and Dynamic Fluorescence. Membr Biochem. 1993 Jan-Mar;10(1):17-27. PubMed PMID: 8510559.
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TY - JOUR T1 - Properties of a phosphatidylcholine derivative of diphenyl hexatriene (DPH-PC) in lymphocyte membranes. A comparison with DPH and the cationic derivative TMA-DPH using static and dynamic fluorescence. AU - Ferretti,G, AU - Tangorra,A, AU - Zolese,G, AU - Curatola,G, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 17 EP - 27 JF - Membrane biochemistry JO - Membr Biochem VL - 10 IS - 1 N2 - Using static and dynamic fluorescence we studied the fluorescence properties of a phosphatidylcholine analog of 1,6-diphenyl-1,3,5-hexatriene (DPH-PC) incorporated in lymphocyte plasma membranes with respect to DPH and its cationic derivative 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH), in order to study if phospholipid derivatives of DPH may be used to investigate structural and physicochemical properties of specific membrane lipid domains. DPH-PC and TMA-DPH showed similar fluorescence polarization values that were significantly higher with respect to DPH, suggesting a localization of the fluorescent portion of DPH-PC in a more ordered region of the membrane which was probably due to the elecrostatic interactions between phospholipid head-groups. The localization of the fluorescent moiety of DPH-PC near the membrane surface was also supported by the study of the fluorescence decay of the three probes using frequency-domain fluorometry. The main lifetime component of DPH-PC was rather similar to that of TMA-DPH (6.74 versus 6.24, ns) but considerably lower with respect to DPH (10.52 ns), in agreement with data obtained from exponential analysis. In lymphocyte membranes obtained from concanavalin A treated cells, a significant decrease of fluorescence polarization has been shown with DPH and its phosphatidylcholine derivative, but not with TMA-DPH. In liposomes obtained from total lipids extracted from lymphocyte membranes, a decrease of fluorescence polarization has been observed only with DPH. Our results suggest that DPH-PC localizes the fluorescent portion of its molecule in membrane microenvironments of different properties with respect to those probed by DPH and TMA-DPH. The use of DPH-phospholipid derivatives and other DPH-probes may represent an useful tool to study plasma membrane heterogeneity in biological membranes. SN - 0149-046X UR - https://www.unboundmedicine.com/medline/citation/8510559/Properties_of_a_phosphatidylcholine_derivative_of_diphenyl_hexatriene__DPH_PC__in_lymphocyte_membranes__A_comparison_with_DPH_and_the_cationic_derivative_TMA_DPH_using_static_and_dynamic_fluorescence_ L2 - https://antibodies.cancer.gov/detail/CPTC-KRAS4B-1 DB - PRIME DP - Unbound Medicine ER -