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Simultaneous determination of nerve-induced adenine nucleotides and nucleosides released from rabbit pulmonary artery.
Anal Biochem. 1993 May 01; 210(2):262-7.AB

Abstract

Electrical field stimulation elicits the release of catecholamines, adenine nucleotides, and adenosine from the rabbit pulmonary artery in a frequency dependent manner. To enhance our ability to investigate the release of endogenous adenine nucleotides and adenosine from this and other biological preparations, a new analytical procedure has been developed. This procedure involves the use of an internal standard, 9-beta-D-arabinofuranosyladenine (IS), the derivatization of ATP, ADP, AMP, adenosine (Ado), and IS with chloroacetaldehyde, the isocratic high-performance liquid chromatographic separation of these ethenopurine derivatives on an Ultron N-phenyl HPLC column, and their detection and quantitation by fluorescence spectroscopy. This procedure has enhanced sensitivity and reliability over existing procedures due to the stability of the chromatographic baseline and the use of an internal standard. When this analytical procedure was utilized to measure the adenine nucleotides and Ado that are released from the rabbit pulmonary artery in response to electrical field stimulation, it was observed that the release of endogenous ATP, ADP, AMP, and Ado exceeded that of endogenous norepinephrine. A molar ratio (6-amino purines:catecholamines) of approximately 2000:1 was obtained at a stimulation frequency of 16 Hz. This observation suggests an important extracellular role for adenine nucleotides and nucleosides in the physiology of vascular tissues.

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Authors+Show Affiliations

Mohri K
Department of Biopharmacy, Meiji College of Pharmacy, Tokyo, Japan.
Takeuchi K
No affiliation info available
Shinozuka K
No affiliation info available
Bjur RA
No affiliation info available
Westfall DP
No affiliation info available

MeSH

AcetaldehydeAdenine NucleotidesAdenosineAdenosine DiphosphateAdenosine MonophosphateAdenosine TriphosphateAnimalsChromatography, High Pressure LiquidDrug StabilityElectric StimulationFluorescent DyesIn Vitro TechniquesMaleNucleosidesPulmonary ArteryRabbitsReference StandardsReproducibility of ResultsVidarabine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8512061

Citation

Mohri, K, et al. "Simultaneous Determination of Nerve-induced Adenine Nucleotides and Nucleosides Released From Rabbit Pulmonary Artery." Analytical Biochemistry, vol. 210, no. 2, 1993, pp. 262-7.
Mohri K, Takeuchi K, Shinozuka K, et al. Simultaneous determination of nerve-induced adenine nucleotides and nucleosides released from rabbit pulmonary artery. Anal Biochem. 1993;210(2):262-7.
Mohri, K., Takeuchi, K., Shinozuka, K., Bjur, R. A., & Westfall, D. P. (1993). Simultaneous determination of nerve-induced adenine nucleotides and nucleosides released from rabbit pulmonary artery. Analytical Biochemistry, 210(2), 262-7.
Mohri K, et al. Simultaneous Determination of Nerve-induced Adenine Nucleotides and Nucleosides Released From Rabbit Pulmonary Artery. Anal Biochem. 1993 May 1;210(2):262-7. PubMed PMID: 8512061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of nerve-induced adenine nucleotides and nucleosides released from rabbit pulmonary artery. AU - Mohri,K, AU - Takeuchi,K, AU - Shinozuka,K, AU - Bjur,R A, AU - Westfall,D P, PY - 1993/5/1/pubmed PY - 1993/5/1/medline PY - 1993/5/1/entrez SP - 262 EP - 7 JF - Analytical biochemistry JO - Anal Biochem VL - 210 IS - 2 N2 - Electrical field stimulation elicits the release of catecholamines, adenine nucleotides, and adenosine from the rabbit pulmonary artery in a frequency dependent manner. To enhance our ability to investigate the release of endogenous adenine nucleotides and adenosine from this and other biological preparations, a new analytical procedure has been developed. This procedure involves the use of an internal standard, 9-beta-D-arabinofuranosyladenine (IS), the derivatization of ATP, ADP, AMP, adenosine (Ado), and IS with chloroacetaldehyde, the isocratic high-performance liquid chromatographic separation of these ethenopurine derivatives on an Ultron N-phenyl HPLC column, and their detection and quantitation by fluorescence spectroscopy. This procedure has enhanced sensitivity and reliability over existing procedures due to the stability of the chromatographic baseline and the use of an internal standard. When this analytical procedure was utilized to measure the adenine nucleotides and Ado that are released from the rabbit pulmonary artery in response to electrical field stimulation, it was observed that the release of endogenous ATP, ADP, AMP, and Ado exceeded that of endogenous norepinephrine. A molar ratio (6-amino purines:catecholamines) of approximately 2000:1 was obtained at a stimulation frequency of 16 Hz. This observation suggests an important extracellular role for adenine nucleotides and nucleosides in the physiology of vascular tissues. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/8512061/Simultaneous_determination_of_nerve_induced_adenine_nucleotides_and_nucleosides_released_from_rabbit_pulmonary_artery_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(83)71194-2 DB - PRIME DP - Unbound Medicine ER -