Tags

Type your tag names separated by a space and hit enter

Platelet-activating factor and its methoxy-analogue ET-18-OCH3 stimulate immediate early gene expression in rat astroglial cultures.
Neurochem Int. 1993 Jun; 22(6):567-74.NI

Abstract

In the present paper we analyzed c-fos and zif/268 expression in rat primary astroglial cell cultures after treatment with Platelet-activating Factor (PAF) and its 2-O-methyl-analogue, 1-O-octadecyl-2-O-methoxy-glycero-3-phosphocholine (ET-18-OCH3). Both compounds, at a dose (2 microM) that did not produce toxic effects on astroglial cells, induced a rapid and transient increase of c-fos and zif/268 mRNA level. Pretreatment of astroglial cells with the PAF antagonist BN50730 (5 microM) 10 min prior to the addition of alkyl-phospholipids almost completely prevented the activation of the immediate early genes. On the contrary triazolam, another PAF inhibitor, did not block PAF induced gene expression when added to the medium at 5 microM concentration. ET-18-OCH3 effect on gene expression is blocked by the same antagonist (BN50730) which is effective in inhibiting PAF effect on astrocytes, suggesting that both substances act through the same binding site. Results obtained support the view that astroglial cells are a cellular target for this lipid mediator, and, like macrophages, respond to its methoxy-analogue.

Authors+Show Affiliations

Institute of Biochemistry, Faculty of Medicine, University of Catania, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8513284

Citation

Dell' Albani, P, et al. "Platelet-activating Factor and Its Methoxy-analogue ET-18-OCH3 Stimulate Immediate Early Gene Expression in Rat Astroglial Cultures." Neurochemistry International, vol. 22, no. 6, 1993, pp. 567-74.
Dell' Albani P, Condorelli DF, Mudò G, et al. Platelet-activating factor and its methoxy-analogue ET-18-OCH3 stimulate immediate early gene expression in rat astroglial cultures. Neurochem Int. 1993;22(6):567-74.
Dell' Albani, P., Condorelli, D. F., Mudò, G., Amico, C., Bindoni, M., & Belluardo, N. (1993). Platelet-activating factor and its methoxy-analogue ET-18-OCH3 stimulate immediate early gene expression in rat astroglial cultures. Neurochemistry International, 22(6), 567-74.
Dell' Albani P, et al. Platelet-activating Factor and Its Methoxy-analogue ET-18-OCH3 Stimulate Immediate Early Gene Expression in Rat Astroglial Cultures. Neurochem Int. 1993;22(6):567-74. PubMed PMID: 8513284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platelet-activating factor and its methoxy-analogue ET-18-OCH3 stimulate immediate early gene expression in rat astroglial cultures. AU - Dell' Albani,P, AU - Condorelli,D F, AU - Mudò,G, AU - Amico,C, AU - Bindoni,M, AU - Belluardo,N, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 567 EP - 74 JF - Neurochemistry international JO - Neurochem Int VL - 22 IS - 6 N2 - In the present paper we analyzed c-fos and zif/268 expression in rat primary astroglial cell cultures after treatment with Platelet-activating Factor (PAF) and its 2-O-methyl-analogue, 1-O-octadecyl-2-O-methoxy-glycero-3-phosphocholine (ET-18-OCH3). Both compounds, at a dose (2 microM) that did not produce toxic effects on astroglial cells, induced a rapid and transient increase of c-fos and zif/268 mRNA level. Pretreatment of astroglial cells with the PAF antagonist BN50730 (5 microM) 10 min prior to the addition of alkyl-phospholipids almost completely prevented the activation of the immediate early genes. On the contrary triazolam, another PAF inhibitor, did not block PAF induced gene expression when added to the medium at 5 microM concentration. ET-18-OCH3 effect on gene expression is blocked by the same antagonist (BN50730) which is effective in inhibiting PAF effect on astrocytes, suggesting that both substances act through the same binding site. Results obtained support the view that astroglial cells are a cellular target for this lipid mediator, and, like macrophages, respond to its methoxy-analogue. SN - 0197-0186 UR - https://www.unboundmedicine.com/medline/citation/8513284/Platelet_activating_factor_and_its_methoxy_analogue_ET_18_OCH3_stimulate_immediate_early_gene_expression_in_rat_astroglial_cultures_ L2 - https://antibodies.cancer.gov/detail/CPTC-FOS-4 DB - PRIME DP - Unbound Medicine ER -