Tags

Type your tag names separated by a space and hit enter

Crystallization and preliminary X-ray investigation of recombinant simian immunodeficiency virus proteinase.
J Mol Biol. 1993 Jun 20; 231(4):1139-42.JM

Abstract

Simian immunodeficiency virus (SIV) proteinase has been crystallized from sodium acetate buffer with sodium chloride as precipitant. The crystals are orthorhombic and the space group is C222(1) with unit cell dimensions a = 32.18 A, b = 62.52 A, c = 95.76 A, alpha = beta = gamma = 90 degrees, indicating a single monomer of 10 kDa in the asymmetric unit. The crystals grow to dimensions of 0.2 mm x 0.2 mm x 0.07 mm within a week and are stable in the X-ray beam for at least 50 hours. A different crystal lattice was observed for SIV proteinase crystallized in the presence of pepstatin. The space group was P2(1)2(1)2(1) with cell dimensions of a = 35.26 A, b = 58.59 A, c = 93.95 A, alpha = beta = gamma = 90 degrees. Diffraction beyond 1.7 A was observed, indicating that a high resolution structure analysis is feasible.

Links

Publisher Full Text

Authors+Show Affiliations

Wilderspin AF
Department of Crystallography, Birbeck College, London, U.K.
Sugrue RJ
No affiliation info available

MeSH

Amino Acid SequenceAspartic Acid EndopeptidasesCrystallizationMolecular Sequence DataRecombinant ProteinsSequence Homology, Amino AcidSimian Immunodeficiency VirusX-Ray Diffraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8515473

Citation

Wilderspin, A F., and R J. Sugrue. "Crystallization and Preliminary X-ray Investigation of Recombinant Simian Immunodeficiency Virus Proteinase." Journal of Molecular Biology, vol. 231, no. 4, 1993, pp. 1139-42.
Wilderspin AF, Sugrue RJ. Crystallization and preliminary X-ray investigation of recombinant simian immunodeficiency virus proteinase. J Mol Biol. 1993;231(4):1139-42.
Wilderspin, A. F., & Sugrue, R. J. (1993). Crystallization and preliminary X-ray investigation of recombinant simian immunodeficiency virus proteinase. Journal of Molecular Biology, 231(4), 1139-42.
Wilderspin AF, Sugrue RJ. Crystallization and Preliminary X-ray Investigation of Recombinant Simian Immunodeficiency Virus Proteinase. J Mol Biol. 1993 Jun 20;231(4):1139-42. PubMed PMID: 8515473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystallization and preliminary X-ray investigation of recombinant simian immunodeficiency virus proteinase. AU - Wilderspin,A F, AU - Sugrue,R J, PY - 1993/6/20/pubmed PY - 1993/6/20/medline PY - 1993/6/20/entrez SP - 1139 EP - 42 JF - Journal of molecular biology JO - J Mol Biol VL - 231 IS - 4 N2 - Simian immunodeficiency virus (SIV) proteinase has been crystallized from sodium acetate buffer with sodium chloride as precipitant. The crystals are orthorhombic and the space group is C222(1) with unit cell dimensions a = 32.18 A, b = 62.52 A, c = 95.76 A, alpha = beta = gamma = 90 degrees, indicating a single monomer of 10 kDa in the asymmetric unit. The crystals grow to dimensions of 0.2 mm x 0.2 mm x 0.07 mm within a week and are stable in the X-ray beam for at least 50 hours. A different crystal lattice was observed for SIV proteinase crystallized in the presence of pepstatin. The space group was P2(1)2(1)2(1) with cell dimensions of a = 35.26 A, b = 58.59 A, c = 93.95 A, alpha = beta = gamma = 90 degrees. Diffraction beyond 1.7 A was observed, indicating that a high resolution structure analysis is feasible. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/8515473/Crystallization_and_preliminary_X_ray_investigation_of_recombinant_simian_immunodeficiency_virus_proteinase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(83)71361-6 DB - PRIME DP - Unbound Medicine ER -