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Preferential interaction of Sec-G with Sec-E stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane.
Biochem Biophys Res Commun 1995; 217(1):217-23BB

Abstract

SecY, SecE and SecG form a membrane part of the protein translocation machinery. A SecG-overproducing plasmid was constructed by placing the secG gene under the control of the tac promoter. From the extent of SecG overproduction, the number of SecG molecules in one normal cell was estimated to be about 1,000, which is similar to those of SecY and SecE. Overproduction of SecG stabilized the overproduction of SecE-C, an unstable truncated derivative of SecE, as effectively as SecY does. SecG overproduction also stabilized the overproduction of SecY. However, the SecG-dependent stabilization of SecY was less potent than the SecE-dependent stabilization. These results indicate that SecG preferentially interacts with SecE, which associates with SecY, the SecG-SecE-SecY complex thus being formed in the cytoplasmic membrane.

Authors+Show Affiliations

Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8526914

Citation

Nishiyama, K, et al. "Preferential Interaction of Sec-G With Sec-E Stabilizes an Unstable Sec-E Derivative in the Escherichia Coli Cytoplasmic Membrane." Biochemical and Biophysical Research Communications, vol. 217, no. 1, 1995, pp. 217-23.
Nishiyama K, Mizushima S, Tokuda H. Preferential interaction of Sec-G with Sec-E stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane. Biochem Biophys Res Commun. 1995;217(1):217-23.
Nishiyama, K., Mizushima, S., & Tokuda, H. (1995). Preferential interaction of Sec-G with Sec-E stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane. Biochemical and Biophysical Research Communications, 217(1), pp. 217-23.
Nishiyama K, Mizushima S, Tokuda H. Preferential Interaction of Sec-G With Sec-E Stabilizes an Unstable Sec-E Derivative in the Escherichia Coli Cytoplasmic Membrane. Biochem Biophys Res Commun. 1995 Dec 5;217(1):217-23. PubMed PMID: 8526914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preferential interaction of Sec-G with Sec-E stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane. AU - Nishiyama,K, AU - Mizushima,S, AU - Tokuda,H, PY - 1995/12/5/pubmed PY - 1995/12/5/medline PY - 1995/12/5/entrez SP - 217 EP - 23 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 217 IS - 1 N2 - SecY, SecE and SecG form a membrane part of the protein translocation machinery. A SecG-overproducing plasmid was constructed by placing the secG gene under the control of the tac promoter. From the extent of SecG overproduction, the number of SecG molecules in one normal cell was estimated to be about 1,000, which is similar to those of SecY and SecE. Overproduction of SecG stabilized the overproduction of SecE-C, an unstable truncated derivative of SecE, as effectively as SecY does. SecG overproduction also stabilized the overproduction of SecY. However, the SecG-dependent stabilization of SecY was less potent than the SecE-dependent stabilization. These results indicate that SecG preferentially interacts with SecE, which associates with SecY, the SecG-SecE-SecY complex thus being formed in the cytoplasmic membrane. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/8526914/Preferential_interaction_of_Sec_G_with_Sec_E_stabilizes_an_unstable_Sec_E_derivative_in_the_Escherichia_coli_cytoplasmic_membrane_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(85)72766-0 DB - PRIME DP - Unbound Medicine ER -