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Thyroid peroxidase autoantibodies and their effects on enzyme activity in patients with systemic lupus erythematosus.
Lupus. 1995 Aug; 4(4):280-5.L

Abstract

Thyroid microsomal antibodies (Ms-Ab) are recently proved to be directed to thyroid peroxidase (TPO). The aim of this study was to investigate whether the sera of patients with systemic lupus erythematosus (SLE) contain anti-TPO antibodies (TPO-Ab) and whether these antibodies influence enzyme activity. Sera from patient with Hashimoto's thyroiditis was also studied. Serum samples were obtained from 37 patients with SLE, 20 patients with Hashimoto's thyroiditis and 20 healthy subjects. TPO-Ab were detected by immunoprecipitation using crude microsomal preparations or enzyme-linked immunoabsorbent assay (ELISA) with recombinant TPO. Positive TPO-Ab by ELISA were found in 11 (61%) of 18 patients with lupus whose serum contained Ms-Ab. Low levels of TPO-Ab also were found in three (16%) of 19 lupus sera that did not contain Ms-Ab. All patients with Hashimoto's thyroiditis had high levels of TPO-Ab in serum. When measured by ELISA, TPO-Ab were highly correlated with the results of a TPO immunoprecipitation assay and with the titers of Ms-Ab in patients with lupus (r = 0.83, n = 18, P < 0.01; r = 0.63, n = 18, P < 0.01) and in Hashimoto's thyroiditis (r = 0.89, n = 20, P < 0.01; r = 0.75, n = 20, P < 0.01). When evaluating the direct influence of TPO-Ab on the activity of TPO, we found no significant inhibition of enzymatic activity in both guaiacol and iodide assays by lupus sera in contrast with sera from Hashimoto's thyroiditis.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Internal Medicine, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8528224

Citation

Tsai, R T., et al. "Thyroid Peroxidase Autoantibodies and Their Effects On Enzyme Activity in Patients With Systemic Lupus Erythematosus." Lupus, vol. 4, no. 4, 1995, pp. 280-5.
Tsai RT, Chang TC, Wang CR, et al. Thyroid peroxidase autoantibodies and their effects on enzyme activity in patients with systemic lupus erythematosus. Lupus. 1995;4(4):280-5.
Tsai, R. T., Chang, T. C., Wang, C. R., Lee, S. L., Wang, C. J., & Tsay, G. J. (1995). Thyroid peroxidase autoantibodies and their effects on enzyme activity in patients with systemic lupus erythematosus. Lupus, 4(4), 280-5.
Tsai RT, et al. Thyroid Peroxidase Autoantibodies and Their Effects On Enzyme Activity in Patients With Systemic Lupus Erythematosus. Lupus. 1995;4(4):280-5. PubMed PMID: 8528224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thyroid peroxidase autoantibodies and their effects on enzyme activity in patients with systemic lupus erythematosus. AU - Tsai,R T, AU - Chang,T C, AU - Wang,C R, AU - Lee,S L, AU - Wang,C J, AU - Tsay,G J, PY - 1995/8/1/pubmed PY - 1995/8/1/medline PY - 1995/8/1/entrez SP - 280 EP - 5 JF - Lupus JO - Lupus VL - 4 IS - 4 N2 - Thyroid microsomal antibodies (Ms-Ab) are recently proved to be directed to thyroid peroxidase (TPO). The aim of this study was to investigate whether the sera of patients with systemic lupus erythematosus (SLE) contain anti-TPO antibodies (TPO-Ab) and whether these antibodies influence enzyme activity. Sera from patient with Hashimoto's thyroiditis was also studied. Serum samples were obtained from 37 patients with SLE, 20 patients with Hashimoto's thyroiditis and 20 healthy subjects. TPO-Ab were detected by immunoprecipitation using crude microsomal preparations or enzyme-linked immunoabsorbent assay (ELISA) with recombinant TPO. Positive TPO-Ab by ELISA were found in 11 (61%) of 18 patients with lupus whose serum contained Ms-Ab. Low levels of TPO-Ab also were found in three (16%) of 19 lupus sera that did not contain Ms-Ab. All patients with Hashimoto's thyroiditis had high levels of TPO-Ab in serum. When measured by ELISA, TPO-Ab were highly correlated with the results of a TPO immunoprecipitation assay and with the titers of Ms-Ab in patients with lupus (r = 0.83, n = 18, P < 0.01; r = 0.63, n = 18, P < 0.01) and in Hashimoto's thyroiditis (r = 0.89, n = 20, P < 0.01; r = 0.75, n = 20, P < 0.01). When evaluating the direct influence of TPO-Ab on the activity of TPO, we found no significant inhibition of enzymatic activity in both guaiacol and iodide assays by lupus sera in contrast with sera from Hashimoto's thyroiditis.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0961-2033 UR - https://www.unboundmedicine.com/medline/citation/8528224/Thyroid_peroxidase_autoantibodies_and_their_effects_on_enzyme_activity_in_patients_with_systemic_lupus_erythematosus_ L2 - http://journals.sagepub.com/doi/full/10.1177/096120339500400408?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -