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Sustained cytokine production and immunophenotypic changes in human neuroblastoma cell lines transduced with a human gamma interferon vector.
Cancer Gene Ther. 1995 Sep; 2(3):171-81.CG

Abstract

The majority of human neuroblastomas express low to undetectable levels of major histocompatibility complex (MHC) class I and II antigens (MHC-I and -II). We studied the effects of gamma interferon (gamma-IFN) transduction on expression of these antigens in six human neuroblastoma cell lines with and without genomic amplification of the N-myc oncogene. All six were stably transduced with an MoMLV-based gamma-IFN retroviral vector (DAh gamma-IFN). G418-resistant cells were assayed for MHC-I, MHC-II, B7-1, and neuroblastoma-associated antigen expression, as well as for gamma-IFN levels in cell culture supernatants. Sustained gamma-IFN production, 2 to > 1000 units/10(6) cells/d, was attained for five of six transduced cell lines and persisted for up to 9 months. This resulted in marked upregulation of MHC-I and MHC-II expression in LA-N-1, LA-N-6, and CHLA-127 cells and moderate upregulation in SK-N-Fi and SK-N-AS cells. One cell line (LA-N-1) had marked induction of MHC-I and MHC-II despite marginal levels of gamma-IFN production. Expression of CD28 ligand B7-1 (as determined by BB1 antibody) remained unchanged in all gamma-IFN-transduced cell lines tested. Expression of several neuroblastoma-associated antigens (NKH1A, 126-4, HSAN 1.2, HNK, 459, and 390) was upregulated in some of the gamma-IFN-transduced cell lines. These results demonstrate that preparation of gamma-IFN expressing neuroblastoma cells for immunotherapeutic purposes is feasible and that gamma-IFN transduction results in phenotypic changes that may improve immunogenicity of human neuroblastoma cells.

Authors+Show Affiliations

UCLA Department of Medicine 90024-1678, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8528960

Citation

Uçar, K, et al. "Sustained Cytokine Production and Immunophenotypic Changes in Human Neuroblastoma Cell Lines Transduced With a Human Gamma Interferon Vector." Cancer Gene Therapy, vol. 2, no. 3, 1995, pp. 171-81.
Uçar K, Seeger RC, Challita PM, et al. Sustained cytokine production and immunophenotypic changes in human neuroblastoma cell lines transduced with a human gamma interferon vector. Cancer Gene Ther. 1995;2(3):171-81.
Uçar, K., Seeger, R. C., Challita, P. M., Watanabe, C. T., Yen, T. L., Morgan, J. P., Amado, R., Chou, E., McCallister, T., & Barber, J. R. (1995). Sustained cytokine production and immunophenotypic changes in human neuroblastoma cell lines transduced with a human gamma interferon vector. Cancer Gene Therapy, 2(3), 171-81.
Uçar K, et al. Sustained Cytokine Production and Immunophenotypic Changes in Human Neuroblastoma Cell Lines Transduced With a Human Gamma Interferon Vector. Cancer Gene Ther. 1995;2(3):171-81. PubMed PMID: 8528960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sustained cytokine production and immunophenotypic changes in human neuroblastoma cell lines transduced with a human gamma interferon vector. A1 - Uçar,K, AU - Seeger,R C, AU - Challita,P M, AU - Watanabe,C T, AU - Yen,T L, AU - Morgan,J P, AU - Amado,R, AU - Chou,E, AU - McCallister,T, AU - Barber,J R, PY - 1995/9/1/pubmed PY - 1995/9/1/medline PY - 1995/9/1/entrez SP - 171 EP - 81 JF - Cancer gene therapy JO - Cancer Gene Ther VL - 2 IS - 3 N2 - The majority of human neuroblastomas express low to undetectable levels of major histocompatibility complex (MHC) class I and II antigens (MHC-I and -II). We studied the effects of gamma interferon (gamma-IFN) transduction on expression of these antigens in six human neuroblastoma cell lines with and without genomic amplification of the N-myc oncogene. All six were stably transduced with an MoMLV-based gamma-IFN retroviral vector (DAh gamma-IFN). G418-resistant cells were assayed for MHC-I, MHC-II, B7-1, and neuroblastoma-associated antigen expression, as well as for gamma-IFN levels in cell culture supernatants. Sustained gamma-IFN production, 2 to > 1000 units/10(6) cells/d, was attained for five of six transduced cell lines and persisted for up to 9 months. This resulted in marked upregulation of MHC-I and MHC-II expression in LA-N-1, LA-N-6, and CHLA-127 cells and moderate upregulation in SK-N-Fi and SK-N-AS cells. One cell line (LA-N-1) had marked induction of MHC-I and MHC-II despite marginal levels of gamma-IFN production. Expression of CD28 ligand B7-1 (as determined by BB1 antibody) remained unchanged in all gamma-IFN-transduced cell lines tested. Expression of several neuroblastoma-associated antigens (NKH1A, 126-4, HSAN 1.2, HNK, 459, and 390) was upregulated in some of the gamma-IFN-transduced cell lines. These results demonstrate that preparation of gamma-IFN expressing neuroblastoma cells for immunotherapeutic purposes is feasible and that gamma-IFN transduction results in phenotypic changes that may improve immunogenicity of human neuroblastoma cells. SN - 0929-1903 UR - https://www.unboundmedicine.com/medline/citation/8528960/Sustained_cytokine_production_and_immunophenotypic_changes_in_human_neuroblastoma_cell_lines_transduced_with_a_human_gamma_interferon_vector_ L2 - http://www.diseaseinfosearch.org/result/5160 DB - PRIME DP - Unbound Medicine ER -