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Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis.
Mycoses. 1995 May-Jun; 38(5-6):177-82.M

Abstract

Blastomyces dermatitidis yeast lysate antigen (T-58, dog isolate) fractions prepared using the Rotofor preparative isoelectric focusing (IEF) cell (Bio-Rad) were compared with B. dermatitidis yeast lysate and filtrate reagents with respect to the detection of antibodies in sera from dogs with blastomycosis, histoplasmosis, coccidioidomycosis, cryptococcosis and aspergillosis. A horseradish peroxidase enzyme immunoassay with Turbo TMB substrate was used in the study. One particular IEF fraction (pH 4.3) was optimal in the assay, and it exhibited greater sensitivity (100%) and specificity (93%) than the lysate or filtrate preparations. The highest degree of cross-reactivity was encountered with the histoplasmosis and coccidioidomycosis specimens and considerably less with the cryptococcosis and aspergillosis sera. Studies are in progress to purify further the optimal IEF fraction.

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Authors+Show Affiliations

Fisher MA
Department of Biological Sciences, Idaho State University, Pocatello, USA.
Bono JL
No affiliation info available
Abuodeh RO
No affiliation info available
Legendre AM
No affiliation info available
Scalarone GM
No affiliation info available

MeSH

AnimalsAntibodies, FungalAntigens, FungalAspergillosisBlastomycosisCoccidioidomycosisCross ReactionsCryptococcosisDog DiseasesDogsEnzyme-Linked Immunosorbent AssayHistoplasmosisIsoelectric FocusingMorphogenesisSensitivity and SpecificityYeasts

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8531928

Citation

Fisher, M A., et al. "Sensitivity and Specificity of an Isoelectric Focusing Fraction of Blastomyces Dermatitidis Yeast Lysate Antigen for the Detection of Canine Blastomycosis." Mycoses, vol. 38, no. 5-6, 1995, pp. 177-82.
Fisher MA, Bono JL, Abuodeh RO, et al. Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis. Mycoses. 1995;38(5-6):177-82.
Fisher, M. A., Bono, J. L., Abuodeh, R. O., Legendre, A. M., & Scalarone, G. M. (1995). Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis. Mycoses, 38(5-6), 177-82.
Fisher MA, et al. Sensitivity and Specificity of an Isoelectric Focusing Fraction of Blastomyces Dermatitidis Yeast Lysate Antigen for the Detection of Canine Blastomycosis. Mycoses. 1995 May-Jun;38(5-6):177-82. PubMed PMID: 8531928.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis. AU - Fisher,M A, AU - Bono,J L, AU - Abuodeh,R O, AU - Legendre,A M, AU - Scalarone,G M, PY - 1995/5/1/pubmed PY - 1995/5/1/medline PY - 1995/5/1/entrez SP - 177 EP - 82 JF - Mycoses JO - Mycoses VL - 38 IS - 5-6 N2 - Blastomyces dermatitidis yeast lysate antigen (T-58, dog isolate) fractions prepared using the Rotofor preparative isoelectric focusing (IEF) cell (Bio-Rad) were compared with B. dermatitidis yeast lysate and filtrate reagents with respect to the detection of antibodies in sera from dogs with blastomycosis, histoplasmosis, coccidioidomycosis, cryptococcosis and aspergillosis. A horseradish peroxidase enzyme immunoassay with Turbo TMB substrate was used in the study. One particular IEF fraction (pH 4.3) was optimal in the assay, and it exhibited greater sensitivity (100%) and specificity (93%) than the lysate or filtrate preparations. The highest degree of cross-reactivity was encountered with the histoplasmosis and coccidioidomycosis specimens and considerably less with the cryptococcosis and aspergillosis sera. Studies are in progress to purify further the optimal IEF fraction. SN - 0933-7407 UR - https://www.unboundmedicine.com/medline/citation/8531928/Sensitivity_and_specificity_of_an_isoelectric_focusing_fraction_of_Blastomyces_dermatitidis_yeast_lysate_antigen_for_the_detection_of_canine_blastomycosis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0933-7407&date=1995&volume=38&issue=5-6&spage=177 DB - PRIME DP - Unbound Medicine ER -