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New indices from the H*2 analyser improve differentiation between heterozygous beta or delta beta thalassaemia and iron-deficiency anaemia.
Clin Lab Haematol 1995; 17(2):151-5CL

Abstract

The two main causes of microcytic and hypochromic anaemia are iron deficiency (IDA) and thalassaemia (THAL) traits. In the Mediterranean area there is a high prevalence of beta and delta-beta THAL minor. The differentiation between these causes of microcytosis can be significantly improved with two new indices, percentage of microcytes (%Mi) and percentage of hypochromic red blood cells (%Hy), and the direct determination of MCHC, provided by the technological advances of the H*2 analyser. Our discriminant analysis, based on the minimization of Wilk's lambda (lambda) criterion, was used to select the best predictive variables to differentiate between IDA and THAL and has resulted in the highest diagnostic efficiency published to date. The discriminant function obtained is a simple linear combination of the following variables: D = 1.145 RBC-0.174 MCV + 0.091 MCHC + 0.787 square root of (%Hy/%Mi)-22.119. The overall correct classification was 97.6% on the training sample (79 THAL and 90 IDA) and 96.7% on a validation sample of microcytic patients (72 THAL and 80 IDA). The sensitivity and diagnostic specificity were 97.5% and 97.8%, respectively, for the training sample, and 95.8% and 97.5% for the control group.

Authors+Show Affiliations

Servei d'Anàlisis Clíniques, Centre d'Assitència Primària Dr Robert, Badalona, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8536418

Citation

Jimenez, C V., et al. "New Indices From the H*2 Analyser Improve Differentiation Between Heterozygous Beta or Delta Beta Thalassaemia and Iron-deficiency Anaemia." Clinical and Laboratory Haematology, vol. 17, no. 2, 1995, pp. 151-5.
Jimenez CV, Minchinela J, Ros J. New indices from the H*2 analyser improve differentiation between heterozygous beta or delta beta thalassaemia and iron-deficiency anaemia. Clin Lab Haematol. 1995;17(2):151-5.
Jimenez, C. V., Minchinela, J., & Ros, J. (1995). New indices from the H*2 analyser improve differentiation between heterozygous beta or delta beta thalassaemia and iron-deficiency anaemia. Clinical and Laboratory Haematology, 17(2), pp. 151-5.
Jimenez CV, Minchinela J, Ros J. New Indices From the H*2 Analyser Improve Differentiation Between Heterozygous Beta or Delta Beta Thalassaemia and Iron-deficiency Anaemia. Clin Lab Haematol. 1995;17(2):151-5. PubMed PMID: 8536418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New indices from the H*2 analyser improve differentiation between heterozygous beta or delta beta thalassaemia and iron-deficiency anaemia. AU - Jimenez,C V, AU - Minchinela,J, AU - Ros,J, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 151 EP - 5 JF - Clinical and laboratory haematology JO - Clin Lab Haematol VL - 17 IS - 2 N2 - The two main causes of microcytic and hypochromic anaemia are iron deficiency (IDA) and thalassaemia (THAL) traits. In the Mediterranean area there is a high prevalence of beta and delta-beta THAL minor. The differentiation between these causes of microcytosis can be significantly improved with two new indices, percentage of microcytes (%Mi) and percentage of hypochromic red blood cells (%Hy), and the direct determination of MCHC, provided by the technological advances of the H*2 analyser. Our discriminant analysis, based on the minimization of Wilk's lambda (lambda) criterion, was used to select the best predictive variables to differentiate between IDA and THAL and has resulted in the highest diagnostic efficiency published to date. The discriminant function obtained is a simple linear combination of the following variables: D = 1.145 RBC-0.174 MCV + 0.091 MCHC + 0.787 square root of (%Hy/%Mi)-22.119. The overall correct classification was 97.6% on the training sample (79 THAL and 90 IDA) and 96.7% on a validation sample of microcytic patients (72 THAL and 80 IDA). The sensitivity and diagnostic specificity were 97.5% and 97.8%, respectively, for the training sample, and 95.8% and 97.5% for the control group. SN - 0141-9854 UR - https://www.unboundmedicine.com/medline/citation/8536418/New_indices_from_the_H_2_analyser_improve_differentiation_between_heterozygous_beta_or_delta_beta_thalassaemia_and_iron_deficiency_anaemia_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0141-9854&date=1995&volume=17&issue=2&spage=151 DB - PRIME DP - Unbound Medicine ER -