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Genetic diversity and population structure of Trypanosoma brucei: clonality versus sexuality.
Mol Biochem Parasitol. 1995 Jun; 72(1-2):89-101.MB

Abstract

Genomic fingerprinting by arbitrarily primed PCR was used to analyze the genetic variability among 59 Trypanosoma brucei stocks representing the three T. brucei subspecies isolated from various hosts and different countries in Africa. 14 oligonucleotide primers revealed 355 polymorphic binary characters which were used for phenetic and phylogenetic analysis and to perform recombination tests exploring the linkage disequilibrium in the sample. There was good concordance between arbitrarily primed PCR polymorphisms and isoenzyme data previously collected for many of the same strains [1]. However, the arbitrarily primed PCR typing was more discerning than multilocus enzyme electrophoresis typing. Phenetic and phylogenetic analysis using arbitrarily primed PCR markers did not confirm T. brucei brucei and T. brucei rhodesiense as separate subspecies, but T. brucei gambiense group I was monophyletic, confirming this group as suitable for the subspecies status. With this exception, there were no clear lineages among the sample, other than clustering of East African stocks and clustering of West African stocks. Some features of the phylogenetic analysis suggested that the population structure was not strictly clonal though recombination tests showed linkage disequilibrium, even in the absence of repeated genotypes. While genotypes appear stable enough for tracking in applied studies, sexuality will impact at the evolutionary time scale, and may be more frequent under some ecological conditions. The arbitrarily primed PCR approach should be an effective and simple approach to follow epidemics and to quantify the role of sexuality in T. brucei populations.

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Authors+Show Affiliations

Mathieu-Daudé F
California Institute of Biological Research, La Jolla 92037, USA.
Stevens J
No affiliation info available
Welsh J
No affiliation info available
Tibayrenc M
No affiliation info available
McClelland M
No affiliation info available

MeSH

AfricaAnimalsAnimals, DomesticBase SequenceCattleDNA FingerprintingDNA, ProtozoanGenetic VariationGenetics, PopulationHumansIsoenzymesMolecular Sequence DataPhylogenyPolymerase Chain ReactionPolymorphism, GeneticProtozoan ProteinsSex CharacteristicsSpecies SpecificityTrypanosomaTrypanosoma brucei bruceiTrypanosoma brucei gambienseTrypanosoma brucei rhodesienseTrypanosomiasis, AfricanTrypanosomiasis, BovineTsetse Flies

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8538703

Citation

Mathieu-Daudé, F, et al. "Genetic Diversity and Population Structure of Trypanosoma Brucei: Clonality Versus Sexuality." Molecular and Biochemical Parasitology, vol. 72, no. 1-2, 1995, pp. 89-101.
Mathieu-Daudé F, Stevens J, Welsh J, et al. Genetic diversity and population structure of Trypanosoma brucei: clonality versus sexuality. Mol Biochem Parasitol. 1995;72(1-2):89-101.
Mathieu-Daudé, F., Stevens, J., Welsh, J., Tibayrenc, M., & McClelland, M. (1995). Genetic diversity and population structure of Trypanosoma brucei: clonality versus sexuality. Molecular and Biochemical Parasitology, 72(1-2), 89-101.
Mathieu-Daudé F, et al. Genetic Diversity and Population Structure of Trypanosoma Brucei: Clonality Versus Sexuality. Mol Biochem Parasitol. 1995;72(1-2):89-101. PubMed PMID: 8538703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic diversity and population structure of Trypanosoma brucei: clonality versus sexuality. AU - Mathieu-Daudé,F, AU - Stevens,J, AU - Welsh,J, AU - Tibayrenc,M, AU - McClelland,M, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 89 EP - 101 JF - Molecular and biochemical parasitology JO - Mol Biochem Parasitol VL - 72 IS - 1-2 N2 - Genomic fingerprinting by arbitrarily primed PCR was used to analyze the genetic variability among 59 Trypanosoma brucei stocks representing the three T. brucei subspecies isolated from various hosts and different countries in Africa. 14 oligonucleotide primers revealed 355 polymorphic binary characters which were used for phenetic and phylogenetic analysis and to perform recombination tests exploring the linkage disequilibrium in the sample. There was good concordance between arbitrarily primed PCR polymorphisms and isoenzyme data previously collected for many of the same strains [1]. However, the arbitrarily primed PCR typing was more discerning than multilocus enzyme electrophoresis typing. Phenetic and phylogenetic analysis using arbitrarily primed PCR markers did not confirm T. brucei brucei and T. brucei rhodesiense as separate subspecies, but T. brucei gambiense group I was monophyletic, confirming this group as suitable for the subspecies status. With this exception, there were no clear lineages among the sample, other than clustering of East African stocks and clustering of West African stocks. Some features of the phylogenetic analysis suggested that the population structure was not strictly clonal though recombination tests showed linkage disequilibrium, even in the absence of repeated genotypes. While genotypes appear stable enough for tracking in applied studies, sexuality will impact at the evolutionary time scale, and may be more frequent under some ecological conditions. The arbitrarily primed PCR approach should be an effective and simple approach to follow epidemics and to quantify the role of sexuality in T. brucei populations. SN - 0166-6851 UR - https://www.unboundmedicine.com/medline/citation/8538703/Genetic_diversity_and_population_structure_of_Trypanosoma_brucei:_clonality_versus_sexuality_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0166-6851(95)00083-D DB - PRIME DP - Unbound Medicine ER -