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Tissue diagnosis of intestinal microsporidiosis using a fluorescent stain with Uvitex 2B.
J Clin Pathol. 1995 Nov; 48(11):1009-10.JC

Abstract

AIMS

To detect intestinal microsporidiosis in paraffin wax embedded biopsy specimens using a fluorescence technique incorporating optical brighteners.

METHODS

Eight HIV infected patients with confirmed intestinal microsporidiosis (six with Enterocytozoon bieneusi, one with Encephalitozoon intestinalis and one with Encephalitozoon cuniculi infection) and 10 without infection were studied. Tissue sections of paraffin wax embedded duodenal biopsy specimens were stained with 1% Uvitex 2B, coded and analysed independently by two investigators.

RESULTS

In all eight cases with confirmed intestinal microsporidian infection, spores could be detected easily in tissue sections using the fluorescence technique. Spores or other elements consistent with microsporidiosis were not found in the 10 patients without infection.

CONCLUSION

Staining of tissue sections from paraffin wax embedded intestinal biopsy specimens with stains incorporating Uvitex 2B is a rapid and easy technique for the diagnosis of intestinal microsporidiosis.

Authors+Show Affiliations

Department of Internal Medicine I, University of Cologne, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Controlled Clinical Trial
Journal Article

Language

eng

PubMed ID

8543621

Citation

Franzen, C, et al. "Tissue Diagnosis of Intestinal Microsporidiosis Using a Fluorescent Stain With Uvitex 2B." Journal of Clinical Pathology, vol. 48, no. 11, 1995, pp. 1009-10.
Franzen C, Müller A, Salzberger B, et al. Tissue diagnosis of intestinal microsporidiosis using a fluorescent stain with Uvitex 2B. J Clin Pathol. 1995;48(11):1009-10.
Franzen, C., Müller, A., Salzberger, B., Fätkenheuer, G., Eidt, S., Mahrle, G., Diehl, V., & Schrappe, M. (1995). Tissue diagnosis of intestinal microsporidiosis using a fluorescent stain with Uvitex 2B. Journal of Clinical Pathology, 48(11), 1009-10.
Franzen C, et al. Tissue Diagnosis of Intestinal Microsporidiosis Using a Fluorescent Stain With Uvitex 2B. J Clin Pathol. 1995;48(11):1009-10. PubMed PMID: 8543621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue diagnosis of intestinal microsporidiosis using a fluorescent stain with Uvitex 2B. AU - Franzen,C, AU - Müller,A, AU - Salzberger,B, AU - Fätkenheuer,G, AU - Eidt,S, AU - Mahrle,G, AU - Diehl,V, AU - Schrappe,M, PY - 1995/11/1/pubmed PY - 1995/11/1/medline PY - 1995/11/1/entrez SP - 1009 EP - 10 JF - Journal of clinical pathology JO - J Clin Pathol VL - 48 IS - 11 N2 - AIMS: To detect intestinal microsporidiosis in paraffin wax embedded biopsy specimens using a fluorescence technique incorporating optical brighteners. METHODS: Eight HIV infected patients with confirmed intestinal microsporidiosis (six with Enterocytozoon bieneusi, one with Encephalitozoon intestinalis and one with Encephalitozoon cuniculi infection) and 10 without infection were studied. Tissue sections of paraffin wax embedded duodenal biopsy specimens were stained with 1% Uvitex 2B, coded and analysed independently by two investigators. RESULTS: In all eight cases with confirmed intestinal microsporidian infection, spores could be detected easily in tissue sections using the fluorescence technique. Spores or other elements consistent with microsporidiosis were not found in the 10 patients without infection. CONCLUSION: Staining of tissue sections from paraffin wax embedded intestinal biopsy specimens with stains incorporating Uvitex 2B is a rapid and easy technique for the diagnosis of intestinal microsporidiosis. SN - 0021-9746 UR - https://www.unboundmedicine.com/medline/citation/8543621/Tissue_diagnosis_of_intestinal_microsporidiosis_using_a_fluorescent_stain_with_Uvitex_2B_ L2 - https://jcp.bmj.com/lookup/pmidlookup?view=long&pmid=8543621 DB - PRIME DP - Unbound Medicine ER -