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Neutralization enzyme-linked immunosorbent assay for evaluation of immunity to measles virus.
Viral Immunol. 1995; 8(1):47-52.VI

Abstract

A neutralization enzyme-linked immunosorbent (Nt-ELISA) assay for determination of protective immunity to measles virus was developed and evaluated. This procedure uses the same initial steps as performed to determine antibody titers by seroneutralization (Nt) test. However, a reduction in virus infectivity by neutralizing antibody was determined by quantitation of viral antigen using ELISA. The serum dilution that resulted in neutralization of 50% of infectious virus could be determined from the absorbance values. To be able to screen a large number of specimens, the conditions of the Nt-ELISA test were adjusted such that negative sera for measles antibodies and the positive ones were clearly distinguished on the basis of a single dilution (1:4). This test showed similar sensitivity (88.3%) and equal specificity as the Nt test when screening 136 serum samples from normal subjects. The estimation of protective antibody titers by Nt and Nt-ELISA methods was strongly correlated (correlation coefficient = 0.91). Thus, the measles Nt-ELISA test is rapid, reproducible, sensitive, and specific for detection of protective measles antibodies.

Authors+Show Affiliations

Instituto de Virología Dr. J.M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8546804

Citation

Nates, S V., et al. "Neutralization Enzyme-linked Immunosorbent Assay for Evaluation of Immunity to Measles Virus." Viral Immunology, vol. 8, no. 1, 1995, pp. 47-52.
Nates SV, Rey GY, Giordano MO, et al. Neutralization enzyme-linked immunosorbent assay for evaluation of immunity to measles virus. Viral Immunol. 1995;8(1):47-52.
Nates, S. V., Rey, G. Y., Giordano, M. O., Depetris, A. R., & Boshell, J. (1995). Neutralization enzyme-linked immunosorbent assay for evaluation of immunity to measles virus. Viral Immunology, 8(1), 47-52.
Nates SV, et al. Neutralization Enzyme-linked Immunosorbent Assay for Evaluation of Immunity to Measles Virus. Viral Immunol. 1995;8(1):47-52. PubMed PMID: 8546804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neutralization enzyme-linked immunosorbent assay for evaluation of immunity to measles virus. AU - Nates,S V, AU - Rey,G Y, AU - Giordano,M O, AU - Depetris,A R, AU - Boshell,J, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 47 EP - 52 JF - Viral immunology JO - Viral Immunol VL - 8 IS - 1 N2 - A neutralization enzyme-linked immunosorbent (Nt-ELISA) assay for determination of protective immunity to measles virus was developed and evaluated. This procedure uses the same initial steps as performed to determine antibody titers by seroneutralization (Nt) test. However, a reduction in virus infectivity by neutralizing antibody was determined by quantitation of viral antigen using ELISA. The serum dilution that resulted in neutralization of 50% of infectious virus could be determined from the absorbance values. To be able to screen a large number of specimens, the conditions of the Nt-ELISA test were adjusted such that negative sera for measles antibodies and the positive ones were clearly distinguished on the basis of a single dilution (1:4). This test showed similar sensitivity (88.3%) and equal specificity as the Nt test when screening 136 serum samples from normal subjects. The estimation of protective antibody titers by Nt and Nt-ELISA methods was strongly correlated (correlation coefficient = 0.91). Thus, the measles Nt-ELISA test is rapid, reproducible, sensitive, and specific for detection of protective measles antibodies. SN - 0882-8245 UR - https://www.unboundmedicine.com/medline/citation/8546804/Neutralization_enzyme_linked_immunosorbent_assay_for_evaluation_of_immunity_to_measles_virus_ L2 - https://www.liebertpub.com/doi/10.1089/vim.1995.8.47?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -